氮化碳与壳聚糖联合作用对沙门氏菌脂质代谢的影响

Q93-33; 目的:以鼠伤寒沙门氏菌为研究对象,从细菌脂质代谢层面探究氮化碳-壳聚糖复合溶胶作用下的杀菌机理.方法:基于透射电子显微镜和蛋白泄露分析氮化碳和壳聚糖联用对细菌细胞壁膜的破坏,并采用脂质代谢组学,通过差异代谢物功能及其调控网络从细菌脂质代谢水平探究杀菌机理.结果:与单独使用氮化碳或壳聚糖相比,复合溶胶对鼠伤寒沙门氏菌细胞壁膜的损伤程度更大.脂质代谢中明显上调和下调的差异代谢物分别为203个和95个,主要集中在与细胞膜构成骨架相关的脂肪酸类、甘油磷脂类和糖脂类中.代谢物功能和调控网络分析表明,复合溶胶处理后,细菌脂肪酸生物合成、甘油磷脂代谢、亚油酸代谢、四烯酸代谢等代谢通路被破坏,...

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Published in食品工业科技 Vol. 45; no. 22; pp. 208 - 216
Main Authors 王艳艳, 肖兴宁, 李建, 马洁乐, 董晓平, 易正凯, 楼乔明, 汪雯
Format Journal Article
LanguageChinese
Published 宁波大学食品科学与工程学院,浙江宁波 315800 01.11.2024
浙江省农业科学院农产品质量安全与营养研究所,浙江 杭州 310012%浙江省农业科学院农产品质量安全与营养研究所,浙江 杭州 310012%宁波大学食品科学与工程学院,浙江宁波 315800%浙江理工大学材料科学与工程学院,浙江 杭州 310021
Subjects
沙门氏菌
Salmonella
chitosan
壳聚糖
光催化
氮化碳
graphene carbon nitride
靶向脂质代谢组学
photocatalysis
targeted lipid metabolomics
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ISSN1002-0306
DOI10.13386/j.issn1002-0306.2023120142

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Abstract Q93-33; 目的:以鼠伤寒沙门氏菌为研究对象,从细菌脂质代谢层面探究氮化碳-壳聚糖复合溶胶作用下的杀菌机理.方法:基于透射电子显微镜和蛋白泄露分析氮化碳和壳聚糖联用对细菌细胞壁膜的破坏,并采用脂质代谢组学,通过差异代谢物功能及其调控网络从细菌脂质代谢水平探究杀菌机理.结果:与单独使用氮化碳或壳聚糖相比,复合溶胶对鼠伤寒沙门氏菌细胞壁膜的损伤程度更大.脂质代谢中明显上调和下调的差异代谢物分别为203个和95个,主要集中在与细胞膜构成骨架相关的脂肪酸类、甘油磷脂类和糖脂类中.代谢物功能和调控网络分析表明,复合溶胶处理后,细菌脂肪酸生物合成、甘油磷脂代谢、亚油酸代谢、四烯酸代谢等代谢通路被破坏,细胞的能量代谢、物质运输和信号传导等生长代谢过程产生紊乱,进而导致细菌死亡.结论:本研究从脂质代谢角度揭示,氮化碳与壳聚糖联合破坏沙门氏菌的代谢通路,特别是与脂质代谢相关的通路,导致细菌代谢紊乱并死亡.这为光催化材料的杀菌机制提供了理论依据.
AbstractList Q93-33; 目的:以鼠伤寒沙门氏菌为研究对象,从细菌脂质代谢层面探究氮化碳-壳聚糖复合溶胶作用下的杀菌机理.方法:基于透射电子显微镜和蛋白泄露分析氮化碳和壳聚糖联用对细菌细胞壁膜的破坏,并采用脂质代谢组学,通过差异代谢物功能及其调控网络从细菌脂质代谢水平探究杀菌机理.结果:与单独使用氮化碳或壳聚糖相比,复合溶胶对鼠伤寒沙门氏菌细胞壁膜的损伤程度更大.脂质代谢中明显上调和下调的差异代谢物分别为203个和95个,主要集中在与细胞膜构成骨架相关的脂肪酸类、甘油磷脂类和糖脂类中.代谢物功能和调控网络分析表明,复合溶胶处理后,细菌脂肪酸生物合成、甘油磷脂代谢、亚油酸代谢、四烯酸代谢等代谢通路被破坏,细胞的能量代谢、物质运输和信号传导等生长代谢过程产生紊乱,进而导致细菌死亡.结论:本研究从脂质代谢角度揭示,氮化碳与壳聚糖联合破坏沙门氏菌的代谢通路,特别是与脂质代谢相关的通路,导致细菌代谢紊乱并死亡.这为光催化材料的杀菌机制提供了理论依据.
Abstract_FL Objective:To investigate the bactericidal mechanism of a composite sol made of carbon nitride and chitosan on Salmonella typhimurium by studying its effects on lipid metabolism.Methods:Transmission electron microscopy and protein leakage concentration were utilized to evaluate the magnitude of cell membrane damage induced by carbon nitride-chitosan composite sol at a cellular level.In addition,lipid metabolomics was employed to investigate the bactericidal process by examining several metabolite functions and regulatory networks associated with bacterial lipid metabolism.Results:The composite sol of carbon nitride-chitosan caused more extensive damage to the cell membrane of Salmonella typhimurium.In lipid metabolism,the significantly up-regulated in 203 metabolites and down-regulated in 95 metabolites.These metabolites were particularly involved in the metabolism of fatty acids,glycerophospholipids,and glycolipids.Metabolite function analysis showed that the composite sol treatment disrupted various metabolic pathways in bacteria.These pathways included fatty acid biosynthesis,glycerol phospholipid metabolism,linoleic acid metabolism,tetraenoic acid metabolism,as well as pathways related to cell energy metabolism,material transport,and signal conduction.These disturbances finally resulted in the demise of the bacteria.Conclusion:This study revealed from the perspective of lipid metabolism that the combination of carbon nitride and chitosan disrupts the metabolic pathways of Salmonella,especially those related to lipid metabolism,leading to bacterial metabolic disruption and death.It provides a theoretical basis for comprehending the mechanisms by which photocatalytic materials exert their antibacterial properties.
Author 李建
易正凯
楼乔明
汪雯
王艳艳
马洁乐
董晓平
肖兴宁
AuthorAffiliation 宁波大学食品科学与工程学院,浙江宁波 315800;浙江省农业科学院农产品质量安全与营养研究所,浙江 杭州 310012%浙江省农业科学院农产品质量安全与营养研究所,浙江 杭州 310012%宁波大学食品科学与工程学院,浙江宁波 315800%浙江理工大学材料科学与工程学院,浙江 杭州 310021
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Author_FL WANG Yanyan
YI Zhengkai
XIAO Xingning
LOU Qiaoming
DONG Xiaoping
MA Jiele
LI Jian
WANG Wen
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DocumentTitle_FL Effects of Carbon Nitride Combined with Chitosan on Lipid Metabolism of Salmonella Typhimurium
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Keywords 沙门氏菌
Salmonella
chitosan
壳聚糖
光催化
氮化碳
graphene carbon nitride
靶向脂质代谢组学
photocatalysis
targeted lipid metabolomics
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浙江省农业科学院农产品质量安全与营养研究所,浙江 杭州 310012%浙江省农业科学院农产品质量安全与营养研究所,浙江 杭州 310012%宁波大学食品科学与工程学院,浙江宁波 315800%浙江理工大学材料科学与工程学院,浙江 杭州 310021
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