表面增强拉曼光谱法灵敏检测鸡肉中拉沙洛西残留

TS207.3; 为提高鸡肉中抗球虫类兽药拉沙洛西(LAS)残留的监控检测效率,本研究利用表面增强拉曼光谱(SERS)技术建立了 LAS残留的灵敏检测方法.本研究首先制备不同粒径的金纳米粒子(AuNPs),根据SERS增强效果选择最优粒径AuNPs作为基底,并利用4-氨基苯硫酚(PATP)作为探针分子评估AuNPs基底重现性;然后通过添加HNO3改变AuNPs聚集程度并增大LAS溶解度,进一步增强SERS信号;最后通过建立标准曲线并进行鸡肉样品加标回收试验对方法进行评价.结果表明,粒径40 nm的AuNPs增强效果较好,1118 cm-1处为LAS的SERS特征峰,基底具有良好的重现性,HNO...

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Published in食品工业科技 Vol. 46; no. 4; pp. 299 - 305
Main Authors 王耀, 孙莹莹, 景钰冰, 杨喜燕, 李燕飞, 黄佳翔, 陈秀金, 李兆周, 曹力, 康怀彬
Format Journal Article
LanguageChinese
Published 河南科技大学食品与生物工程学院/食品加工与安全国家级实验教学示范中心/河南省食品绿色加工与质量安全控制国际联合实验室,河南洛阳 471000 01.02.2025
Subjects
lasalocid
chicken
detection
金纳米粒子
gold nanoparticles
检测
表面增强拉曼光谱
拉沙洛西
鸡肉
surface enhanced Raman spectroscopy
Online AccessGet full text
ISSN1002-0306
DOI10.13386/j.issn1002-0306.2024030216

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Abstract TS207.3; 为提高鸡肉中抗球虫类兽药拉沙洛西(LAS)残留的监控检测效率,本研究利用表面增强拉曼光谱(SERS)技术建立了 LAS残留的灵敏检测方法.本研究首先制备不同粒径的金纳米粒子(AuNPs),根据SERS增强效果选择最优粒径AuNPs作为基底,并利用4-氨基苯硫酚(PATP)作为探针分子评估AuNPs基底重现性;然后通过添加HNO3改变AuNPs聚集程度并增大LAS溶解度,进一步增强SERS信号;最后通过建立标准曲线并进行鸡肉样品加标回收试验对方法进行评价.结果表明,粒径40 nm的AuNPs增强效果较好,1118 cm-1处为LAS的SERS特征峰,基底具有良好的重现性,HNO3最优添加浓度为0.5 mol/L.本方法的线性范围为0.55x10-6～0.55 mg/mL,检测限为0.23 ng/mL,鸡肉样品的加标回收率为91.9％～107.3％,相对标准偏差为3.6％～5.7％,与国标法(GB31613.5-2022)的检测结果一致.本研究为LAS残留的灵敏检测提供了新方法,对于其他兽药残留的SERS检测方法研究具有参考价值.
AbstractList TS207.3; 为提高鸡肉中抗球虫类兽药拉沙洛西(LAS)残留的监控检测效率,本研究利用表面增强拉曼光谱(SERS)技术建立了 LAS残留的灵敏检测方法.本研究首先制备不同粒径的金纳米粒子(AuNPs),根据SERS增强效果选择最优粒径AuNPs作为基底,并利用4-氨基苯硫酚(PATP)作为探针分子评估AuNPs基底重现性;然后通过添加HNO3改变AuNPs聚集程度并增大LAS溶解度,进一步增强SERS信号;最后通过建立标准曲线并进行鸡肉样品加标回收试验对方法进行评价.结果表明,粒径40 nm的AuNPs增强效果较好,1118 cm-1处为LAS的SERS特征峰,基底具有良好的重现性,HNO3最优添加浓度为0.5 mol/L.本方法的线性范围为0.55x10-6～0.55 mg/mL,检测限为0.23 ng/mL,鸡肉样品的加标回收率为91.9％～107.3％,相对标准偏差为3.6％～5.7％,与国标法(GB31613.5-2022)的检测结果一致.本研究为LAS残留的灵敏检测提供了新方法,对于其他兽药残留的SERS检测方法研究具有参考价值.
Abstract_FL In order to improve the efficiency of monitoring and detecting the residues of the anticoccidial veterinary drug lasalocid(LAS)in chicken,a sensitive method for the determination of LAS residues was established by surface-enhanced Raman spectroscopy(SERS).In this study,gold nanoparticles(AuNPs)with different particle sizes were first prepared,AuNPs with the optimal particle size were selected as the substrate according to SERS effect,and 4-aminophenthiophenol(PATP)was used as the probe molecule to evaluate the recurrence of AuNPs substrate.Then,by adding HNO3,AuNPs aggregation degree was changed and LAS solubility was increased to further enhance SERS signal.Finally,the method was evaluated by establishing the standard curve and the recovery test of chicken sample.Results showed that AuNPs with a particle size of 40 nm had good enhancement effect,the SERS characteristic peak of LAS was at 1118 cm-1,the AuNPs substrate had good reproducibility,and the optimal addition concentration of HNO3 was 0.5 mol/L.The linear range of this method was 0.55×10-6～0.55 mg/mL,the detection limit was 0.23 ng/mL,the recovery rate of chicken samples was 91.9％～107.3％,and the relative standard deviation was 3.6％～5.7％,which was consistent with the detection results of the national standard method(GB31613.5-2022).This study provides a new method for the sensitive detection of LAS residues,and has reference value for the study of SERS detection method of other veterinary drug residues.
Author 李燕飞
陈秀金
王耀
黄佳翔
康怀彬
景钰冰
李兆周
孙莹莹
曹力
杨喜燕
AuthorAffiliation 河南科技大学食品与生物工程学院/食品加工与安全国家级实验教学示范中心/河南省食品绿色加工与质量安全控制国际联合实验室,河南洛阳 471000
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Author_FL SUN Yingying
LI Yanfei
CAO Li
LI Zhaozhou
YANG Xiyan
HUANG Jiaxiang
WANG Yao
JING Yubing
KANG Huaibin
CHEN Xiujin
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DocumentTitle_FL Sensitive Detection of Lasalocid Residue in Chicken by Surface Enhanced Raman Spectroscopy
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Keywords lasalocid
chicken
detection
金纳米粒子
gold nanoparticles
检测
表面增强拉曼光谱
拉沙洛西
鸡肉
surface enhanced Raman spectroscopy
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PublicationTitle 食品工业科技
PublicationTitle_FL Science and Technology of Food Industry
PublicationYear 2025
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Snippet TS207.3; 为提高鸡肉中抗球虫类兽药拉沙洛西(LAS)残留的监控检测效率,本研究利用表面增强拉曼光谱(SERS)技术建立了...
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Title 表面增强拉曼光谱法灵敏检测鸡肉中拉沙洛西残留
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