荧光定量PCR技术在食品快速检测中的应用

TS207.3; 荧光定量聚合酶链式反应(real-time quantitative polymerase chain reaction,qPCR)是一种将聚合酶链式反应(polymerase chain reaction,PCR)扩增与实时检测相结合的技术,也是近年来食品快速检测技术的研究热点之一.与传统PCR技术相比,它具有耗时短、操作简单、灵敏度高、特异性强等优势.本文简要概述了荧光定量PCR技术的基本原理、发展历程、分类及特点,综述了荧光定量PCR技术在食源性致病菌、掺杂掺假、转基因食品、过敏原、食源性寄生虫、可食用昆虫等食品检测中的应用,并对荧光定量PCR技术及其在食品快速检测中应...

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Published in食品工业科技 Vol. 42; no. 7; pp. 366 - 373
Main Authors 丁博群, 刘珊娜
Format Journal Article
LanguageChinese
Published 天津农学院食品科学与生物工程学院,天津300392 01.04.2021
Subjects
掺杂掺假
荧光定量聚合酶链式反应(qPCR)
快速检测
食源性病原菌
过敏原
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Abstract TS207.3; 荧光定量聚合酶链式反应(real-time quantitative polymerase chain reaction,qPCR)是一种将聚合酶链式反应(polymerase chain reaction,PCR)扩增与实时检测相结合的技术,也是近年来食品快速检测技术的研究热点之一.与传统PCR技术相比,它具有耗时短、操作简单、灵敏度高、特异性强等优势.本文简要概述了荧光定量PCR技术的基本原理、发展历程、分类及特点,综述了荧光定量PCR技术在食源性致病菌、掺杂掺假、转基因食品、过敏原、食源性寄生虫、可食用昆虫等食品检测中的应用,并对荧光定量PCR技术及其在食品快速检测中应用的前景进行了展望.
AbstractList TS207.3; 荧光定量聚合酶链式反应(real-time quantitative polymerase chain reaction,qPCR)是一种将聚合酶链式反应(polymerase chain reaction,PCR)扩增与实时检测相结合的技术,也是近年来食品快速检测技术的研究热点之一.与传统PCR技术相比,它具有耗时短、操作简单、灵敏度高、特异性强等优势.本文简要概述了荧光定量PCR技术的基本原理、发展历程、分类及特点,综述了荧光定量PCR技术在食源性致病菌、掺杂掺假、转基因食品、过敏原、食源性寄生虫、可食用昆虫等食品检测中的应用,并对荧光定量PCR技术及其在食品快速检测中应用的前景进行了展望.
Author 丁博群
刘珊娜
AuthorAffiliation 天津农学院食品科学与生物工程学院,天津300392
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LIU Shanna
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DocumentTitle_FL Application of Real-time Quantitative PCR Technology in Food Rapid Detection
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Keywords 掺杂掺假
荧光定量聚合酶链式反应(qPCR)
快速检测
食源性病原菌
过敏原
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Title 荧光定量PCR技术在食品快速检测中的应用
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