硫酸化修饰对小球藻胞内多糖抗氧化及抗肿瘤活性的影响

TS201.2; 为探究小球藻多糖硫酸化修饰对生物活性影响,本研究采用小球藻(Chlorella sp.22)为实验材料,提取胞内多糖经DEAE-52纤维素阴离子交换柱分离纯化得到纯化多糖HDB-1,对纯化多糖HDB-1进行硫酸化修饰得SHDB-1,对硫酸化修饰前后多糖进行抗氧化活性和抗肿瘤活性研究.结果表明,纯化组分HDB-1以α-呋喃糖为主.用三氧化硫-吡啶法对HDB-1进行硫酸化修饰得到SHDB-1,其取代度为1.046.抗氧化活性结果表明,HDB-1与SHDB-1的DPPH自由基清除率IC50(半抑制浓度)值分别为29.28 mg/mL和14.49 mg/mL,羟自由基清除率IC50值...

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Published in食品工业科技 Vol. 44; no. 7; pp. 82 - 88
Main Authors 孙寒, 藏颖, 刘松, 郭丽珍, 李项娜, 黄磊恒, 宁紫月, 徐程浩, 刘红全
Format Journal Article
LanguageChinese
Published 广西民族大学海洋与生物技术学院,广西多糖材料与改性重点实验室,广西民族大学海洋生物资源保护与利用重点实验室,广西南宁 530007 01.04.2023
Subjects
硫酸化修饰
胞内多糖
抗氧化活性
抗肿瘤活性
小球藻
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ISSN1002-0306
DOI10.13386/j.issn1002-0306.2022060015

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Abstract TS201.2; 为探究小球藻多糖硫酸化修饰对生物活性影响,本研究采用小球藻(Chlorella sp.22)为实验材料,提取胞内多糖经DEAE-52纤维素阴离子交换柱分离纯化得到纯化多糖HDB-1,对纯化多糖HDB-1进行硫酸化修饰得SHDB-1,对硫酸化修饰前后多糖进行抗氧化活性和抗肿瘤活性研究.结果表明,纯化组分HDB-1以α-呋喃糖为主.用三氧化硫-吡啶法对HDB-1进行硫酸化修饰得到SHDB-1,其取代度为1.046.抗氧化活性结果表明,HDB-1与SHDB-1的DPPH自由基清除率IC50(半抑制浓度)值分别为29.28 mg/mL和14.49 mg/mL,羟自由基清除率IC50值分别为36.75 mg/mL和28.59 mg/mL,可知SHDB-1抗氧化效果优于HDB-1.抗肿瘤结果表明,HDB-1及SHDB-1在1 mg/mL浓度下对细胞无毒性,HDB-1与SHDB-1的IC50值分别为960.16μg/mL及658.19μg/mL,可知SHDB-1抑制宫颈癌细胞Hela增殖效果优于HDB-1.以上结果表明,硫酸化修饰的小球藻胞内多糖SHDB-1与HDB-1相比抗氧化活性及体外抑制癌细胞增殖率都有明显提高.
AbstractList TS201.2; 为探究小球藻多糖硫酸化修饰对生物活性影响,本研究采用小球藻(Chlorella sp.22)为实验材料,提取胞内多糖经DEAE-52纤维素阴离子交换柱分离纯化得到纯化多糖HDB-1,对纯化多糖HDB-1进行硫酸化修饰得SHDB-1,对硫酸化修饰前后多糖进行抗氧化活性和抗肿瘤活性研究.结果表明,纯化组分HDB-1以α-呋喃糖为主.用三氧化硫-吡啶法对HDB-1进行硫酸化修饰得到SHDB-1,其取代度为1.046.抗氧化活性结果表明,HDB-1与SHDB-1的DPPH自由基清除率IC50(半抑制浓度)值分别为29.28 mg/mL和14.49 mg/mL,羟自由基清除率IC50值分别为36.75 mg/mL和28.59 mg/mL,可知SHDB-1抗氧化效果优于HDB-1.抗肿瘤结果表明,HDB-1及SHDB-1在1 mg/mL浓度下对细胞无毒性,HDB-1与SHDB-1的IC50值分别为960.16μg/mL及658.19μg/mL,可知SHDB-1抑制宫颈癌细胞Hela增殖效果优于HDB-1.以上结果表明,硫酸化修饰的小球藻胞内多糖SHDB-1与HDB-1相比抗氧化活性及体外抑制癌细胞增殖率都有明显提高.
Author 孙寒
郭丽珍
藏颖
李项娜
宁紫月
徐程浩
刘松
刘红全
黄磊恒
AuthorAffiliation 广西民族大学海洋与生物技术学院,广西多糖材料与改性重点实验室,广西民族大学海洋生物资源保护与利用重点实验室,广西南宁 530007
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Author_FL HUANG Leiheng
NING Ziyue
SUN Han
LIU Song
LI Xiangna
LIU Hongquan
XU Chenghao
ZANG Ying
GUO Lizhen
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Keywords 硫酸化修饰
胞内多糖
抗氧化活性
抗肿瘤活性
小球藻
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Publisher 广西民族大学海洋与生物技术学院,广西多糖材料与改性重点实验室,广西民族大学海洋生物资源保护与利用重点实验室,广西南宁 530007
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Title 硫酸化修饰对小球藻胞内多糖抗氧化及抗肿瘤活性的影响
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