亚麻LuWRI1a在旱盐胁迫响应中的功能分析
AP2/ERF 转录因子家族参与植物对生物和非生物胁迫响应的调控.前期我们从亚麻中克隆了一个WRINKLED1 的同源基因LuWRI1a,蛋白序列分析发现,LuWRI1a包含 2 个AP2 的DNA结合域,属于AP2/ERF转录因子家族.对LuWRI1a的顺式作用元件进行分析发现,pLuWRI1a包含响应光、干旱、低温和激素等多个非生物胁迫应激元件.本研究以亚麻栽培品种陇亚 10 号和LuWRI1a过表达转基因纯合株系为试验材料,用 200 mmol L-1 NaCl营养液和 25%PEG营养液模拟盐胁迫和干旱胁迫处理.结果表明,在盐胁迫和干旱胁迫处理后,转基因植株的相对株高、主根长度、侧根数...
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| Published in | 作物学报 Vol. 50; no. 7; pp. 1750 - 1761 |
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| Main Authors | , , , , , , , , , , |
| Format | Journal Article |
| Language | Chinese |
| Published |
甘肃省农业科学院作物研究所,甘肃兰州 730070
12.07.2024
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0496-3490 |
| DOI | 10.3724/SP.J.1006.2024.34171 |
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| Abstract | AP2/ERF 转录因子家族参与植物对生物和非生物胁迫响应的调控.前期我们从亚麻中克隆了一个WRINKLED1 的同源基因LuWRI1a,蛋白序列分析发现,LuWRI1a包含 2 个AP2 的DNA结合域,属于AP2/ERF转录因子家族.对LuWRI1a的顺式作用元件进行分析发现,pLuWRI1a包含响应光、干旱、低温和激素等多个非生物胁迫应激元件.本研究以亚麻栽培品种陇亚 10 号和LuWRI1a过表达转基因纯合株系为试验材料,用 200 mmol L-1 NaCl营养液和 25%PEG营养液模拟盐胁迫和干旱胁迫处理.结果表明,在盐胁迫和干旱胁迫处理后,转基因植株的相对株高、主根长度、侧根数目及叶片数均升高;3 种抗氧化酶的活性均显著高于对照,而 MDA含量低于对照;非生物胁迫响应基因LuAREB、LuDREB、LuLEA和LuNCED的表达水平均上调.通过探究LuWRI1a在逆境胁迫下的生物学功能发现,LuWRI1a 通过抵抗盐胁迫和干旱胁迫对亚麻生长的抑制,增强活性氧清除能力、减轻膜脂的氧化损伤,激活逆境胁迫响应基因的表达等途径,增强了亚麻的耐逆性.综上所述,LuWRI1a可能是一个多功能基因,它不仅参与脂肪酸合成代谢途径,还有可能参与植物非生物胁迫信号途径.本研究为亚麻耐逆品种改良提供了新的基因资源. |
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| AbstractList | AP2/ERF 转录因子家族参与植物对生物和非生物胁迫响应的调控.前期我们从亚麻中克隆了一个WRINKLED1 的同源基因LuWRI1a,蛋白序列分析发现,LuWRI1a包含 2 个AP2 的DNA结合域,属于AP2/ERF转录因子家族.对LuWRI1a的顺式作用元件进行分析发现,pLuWRI1a包含响应光、干旱、低温和激素等多个非生物胁迫应激元件.本研究以亚麻栽培品种陇亚 10 号和LuWRI1a过表达转基因纯合株系为试验材料,用 200 mmol L-1 NaCl营养液和 25%PEG营养液模拟盐胁迫和干旱胁迫处理.结果表明,在盐胁迫和干旱胁迫处理后,转基因植株的相对株高、主根长度、侧根数目及叶片数均升高;3 种抗氧化酶的活性均显著高于对照,而 MDA含量低于对照;非生物胁迫响应基因LuAREB、LuDREB、LuLEA和LuNCED的表达水平均上调.通过探究LuWRI1a在逆境胁迫下的生物学功能发现,LuWRI1a 通过抵抗盐胁迫和干旱胁迫对亚麻生长的抑制,增强活性氧清除能力、减轻膜脂的氧化损伤,激活逆境胁迫响应基因的表达等途径,增强了亚麻的耐逆性.综上所述,LuWRI1a可能是一个多功能基因,它不仅参与脂肪酸合成代谢途径,还有可能参与植物非生物胁迫信号途径.本研究为亚麻耐逆品种改良提供了新的基因资源. |
| Abstract_FL | The AP2/ERF family of transcription factors is involved in the regulation of plant responses to biotic and abiotic stresses.Previously,we cloned LuWRI1a,a WRINKLED1 homologous gene from flax.Protein sequence analysis showed that LuWRI1a contained two AP2 DNA-binding domains and belonged to the AP2/ERF transcription factor family.The cis-acting elements of LuWRI1a were analyzed that pLuWRI1a was found containing multiple abiotic stress elements in response to light,drought,low temperature and hormones.In this study,the flax cultivar Longya 10 and LuWRI1a overexpression transgenic pure lines were used as the experimental materials,and salt stress and drought stress treatments were simulated with 200 mmol L-1 NaCl nutrient solution and 25%PEG nutrient solution.The results showed that the relative plant height,primary root length,lat-eral root number,and leaf number of transgenic plants were elevated after salt and drought stress treatments.The activities of three antioxidant enzymes were significantly higher than the control,while MDA content was lower.The relative expression lev-els of the abiotic stress-responsive genes,LuAREB,LuDREB,LuLEA,and LuNCED,were up-regulated.By exploring the biologi-cal function of LuWRI1a under adversity stress,it was found that LuWRI1a enhanced flax tolerance by resisting the inhibition of flax growth by salt stress and drought stress,enhancing the scavenging ability of reactive oxygen species,reducing the oxidative damage of membrane lipids,and activating the expression of adversity stress response genes.In summary,LuWRI1a may be a multifunctional gene,which was not only involved in fatty acid synthesis and metabolism pathway,but also may be involved in plant abiotic stress signaling pathway.This study provides a new genetic resource for the improvement of stress-tolerant varieties of flax. |
| Author | 李闻娟 党照 赵玮 张建平 王琰 赵丽蓉 李雯 齐燕妮 谢亚萍 徐晨梦 王利民 |
| AuthorAffiliation | 甘肃省农业科学院作物研究所,甘肃兰州 730070 |
| AuthorAffiliation_xml | – name: 甘肃省农业科学院作物研究所,甘肃兰州 730070 |
| Author_FL | DANG Zhao WANG Yan ZHAO Li-Rong ZHANG Jian-Ping QI Yan-Ni XU Chen-Meng ZHAO Wei LI Wen LI Wen-Juan XIE Ya-Ping WANG Li-Min |
| Author_FL_xml | – sequence: 1 fullname: LI Wen-Juan – sequence: 2 fullname: WANG Li-Min – sequence: 3 fullname: QI Yan-Ni – sequence: 4 fullname: ZHAO Wei – sequence: 5 fullname: XIE Ya-Ping – sequence: 6 fullname: DANG Zhao – sequence: 7 fullname: ZHAO Li-Rong – sequence: 8 fullname: LI Wen – sequence: 9 fullname: XU Chen-Meng – sequence: 10 fullname: WANG Yan – sequence: 11 fullname: ZHANG Jian-Ping |
| Author_xml | – sequence: 1 fullname: 李闻娟 – sequence: 2 fullname: 王利民 – sequence: 3 fullname: 齐燕妮 – sequence: 4 fullname: 赵玮 – sequence: 5 fullname: 谢亚萍 – sequence: 6 fullname: 党照 – sequence: 7 fullname: 赵丽蓉 – sequence: 8 fullname: 李雯 – sequence: 9 fullname: 徐晨梦 – sequence: 10 fullname: 王琰 – sequence: 11 fullname: 张建平 |
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| Keywords | flax drought stress 盐胁迫 干旱胁迫 LuWRI1a 功能分析 functional analysis salt stress 亚麻 |
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| Title | 亚麻LuWRI1a在旱盐胁迫响应中的功能分析 |
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