新生儿持续性肺动脉高压动物模型建立方法的系统性文献分析
目的:新生儿持续性肺动脉高压(persistent pulmonary hypertension of the newborn,PPHN)发病机制复杂,疾病危险因素尚未完全阐明,构建符合PPHN病理生理特征的动物模型对研究其发病机制和治疗方法具有重要意义.本研究旨在通过系统性文献检索,总结并分析PPHN动物模型的应用领域、造模要素及成模标准,以期为建立科学规范且经济易行的动物模型提供参考.方法:在中文数据库(中国知网、万方、维普和中国生物医学文献服务系统)及英文数据库(PubMed、Web of Science和Embase)中检索从建库至2023年3月收录的PPHN动物实验性研究,整理归纳动...
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| Published in | 中南大学学报（医学版） Vol. 49; no. 9; pp. 1477 - 1494 |
|---|---|
| Main Authors | , , , , |
| Format | Journal Article |
| Language | Chinese |
| Published |
中南大学湘雅药学院药理学系,长沙 410078%中南大学湘雅医院药学部,长沙 410008%中南大学图书馆,长沙 410013
2024
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1672-7347 |
| DOI | 10.11817/j.issn.1672-7347.2024.230539 |
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| Abstract | 目的:新生儿持续性肺动脉高压(persistent pulmonary hypertension of the newborn,PPHN)发病机制复杂,疾病危险因素尚未完全阐明,构建符合PPHN病理生理特征的动物模型对研究其发病机制和治疗方法具有重要意义.本研究旨在通过系统性文献检索,总结并分析PPHN动物模型的应用领域、造模要素及成模标准,以期为建立科学规范且经济易行的动物模型提供参考.方法:在中文数据库(中国知网、万方、维普和中国生物医学文献服务系统)及英文数据库(PubMed、Web of Science和Embase)中检索从建库至2023年3月收录的PPHN动物实验性研究,整理归纳动物模型应用领域,实验动物种类及具体品种,造模方法及其原理、具体操作、成模时间、所用动物类型和初始年龄,以及成模标准等信息,建立数据库.对自建数据库进行统计分析,并绘制图表.结果:共检索到PPHN动物模型相关文献1157篇,按照纳入和排除标准筛选后,最终纳入174篇.PPHN动物模型主要用于病理生理特征和机制研究(47.70％).PPHN造模动物包括(累计频次由高到低):羊、猪、大鼠、牛和小鼠.PPHN造模方式可分为缺氧暴露法(44.69％)、产前动脉导管(ductus arteriosus,DA)缩窄法(41.34％)、制剂诱导法(8.94％)和联合诱导法(缺氧暴露联合吲哚美辛)(5.03％)4大类.各类造模方法使用的动物种类及年龄不同,产前DA缩窄法均使用胎羊造模.各类造模方法的成模时间存在差异.缺氧暴露法的成模时间通常超过3d,产前DA缩窄法的中位成模时间为8.5 d,联合诱导法为3d.PPHN的成模标准可从解剖结构、组织学特征、血流动力学参数、血气分析和其他生理指标5个维度划分.最常用的成模标准是右心室肥厚和肺动脉压显著升高.结论:目前PPHN动物造模方法种类繁多,但各类方法均有其优点和局限性.研究者需结合自身研究特点、经费预算等因素选择造模方式.未来仍需进一步探索该领域,以建立具有良好科学性、经济性和可重复性的PPHN动物模型. |
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| AbstractList | 目的:新生儿持续性肺动脉高压(persistent pulmonary hypertension of the newborn,PPHN)发病机制复杂,疾病危险因素尚未完全阐明,构建符合PPHN病理生理特征的动物模型对研究其发病机制和治疗方法具有重要意义.本研究旨在通过系统性文献检索,总结并分析PPHN动物模型的应用领域、造模要素及成模标准,以期为建立科学规范且经济易行的动物模型提供参考.方法:在中文数据库(中国知网、万方、维普和中国生物医学文献服务系统)及英文数据库(PubMed、Web of Science和Embase)中检索从建库至2023年3月收录的PPHN动物实验性研究,整理归纳动物模型应用领域,实验动物种类及具体品种,造模方法及其原理、具体操作、成模时间、所用动物类型和初始年龄,以及成模标准等信息,建立数据库.对自建数据库进行统计分析,并绘制图表.结果:共检索到PPHN动物模型相关文献1157篇,按照纳入和排除标准筛选后,最终纳入174篇.PPHN动物模型主要用于病理生理特征和机制研究(47.70％).PPHN造模动物包括(累计频次由高到低):羊、猪、大鼠、牛和小鼠.PPHN造模方式可分为缺氧暴露法(44.69％)、产前动脉导管(ductus arteriosus,DA)缩窄法(41.34％)、制剂诱导法(8.94％)和联合诱导法(缺氧暴露联合吲哚美辛)(5.03％)4大类.各类造模方法使用的动物种类及年龄不同,产前DA缩窄法均使用胎羊造模.各类造模方法的成模时间存在差异.缺氧暴露法的成模时间通常超过3d,产前DA缩窄法的中位成模时间为8.5 d,联合诱导法为3d.PPHN的成模标准可从解剖结构、组织学特征、血流动力学参数、血气分析和其他生理指标5个维度划分.最常用的成模标准是右心室肥厚和肺动脉压显著升高.结论:目前PPHN动物造模方法种类繁多,但各类方法均有其优点和局限性.研究者需结合自身研究特点、经费预算等因素选择造模方式.未来仍需进一步探索该领域,以建立具有良好科学性、经济性和可重复性的PPHN动物模型. |
| Abstract_FL | Objective:Persistent pulmonary hypertension of the newborn(PPHN)is a complex disease with poorly understood mechanisms and risk factors.Developing animal models that reflect the pathophysiological characteristics of PPHN is critical for studying its pathogenesis and treatment strategies.This study aims to analyze the applications,methodologies,and evaluation criteria of PPHN animal models through systematic literature analysis,and to provide guidelines for establishing scientific,cost-effective,and reproducible models.
Methods:Relevant studies published until March 2023 were retrieved from Chinese databases(CNKI,Wanfang,VIP,and SinoMed)and English databases(PubMed,Web of Science,and Embase).Data were extracted on the applications,species,modeling methods and their principles,specific procedures,species and ages of animals used,and time to model establishment,and criteria for evaluating successful model establishment.A database was created and statistical analyses were performed using Excel 2021,with results presented as charts.
Results:A total of 1 157 studies were identified,and 174 met the inclusion criteria.PPHN animal models were primarily used for pathophysiological and mechanistic research(47.70％).The species used including sheep,pigs,rats,cows,and mice,ranked by frequency of use.Modeling methods were categorized into 4 types:Hypoxia exposure(44.69％),prenatal ductus arteriosus(DA)constriction(41.34％),pharmacological induction(8.94％),and combined induction(hypoxia and indomethacin,5.03％).Each method was associated with specific species and ages.Prenatal DA constriction was exclusively performed in fetal sheep.The time to model establishment varied:Hypoxia exposure typically required＞3 days,prenatal DA constriction had a median of 8.5 days,and combined induction took 3 days.Criteria for evaluating successful model establishment were categorized into 5 dimensions:Anatomical structure,histological features,hemodynamic parameters,blood gas analysis,and other physiological indicators.The most common criteria were"right ventricular hypertrophy"and"significant pulmonary arterial pressure elevation".
Conclusion:Current methods for modeling PPHN in animals are diverse,each with its strengths and limitations.Researchers should select the method most suitable for their study objectives and budget.Further exploration is needed to develop scientifically robust,cost-effective,and reproducible animal models for PPHN. |
| Author | 罗平 胡长平 孙刘琴 周姝含 蔡明远 |
| AuthorAffiliation | 中南大学湘雅药学院药理学系,长沙 410078%中南大学湘雅医院药学部,长沙 410008%中南大学图书馆,长沙 410013 |
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| Author_FL | SUN Liuqin CAI Mingyuan ZHOU Shuhan HU Changping LUO Ping |
| Author_FL_xml | – sequence: 1 fullname: CAI Mingyuan – sequence: 2 fullname: ZHOU Shuhan – sequence: 3 fullname: LUO Ping – sequence: 4 fullname: HU Changping – sequence: 5 fullname: SUN Liuqin |
| Author_xml | – sequence: 1 fullname: 蔡明远 – sequence: 2 fullname: 周姝含 – sequence: 3 fullname: 罗平 – sequence: 4 fullname: 胡长平 – sequence: 5 fullname: 孙刘琴 |
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