人工设计短肽PF1和PF2的克隆表达及其对葡萄糖氧化酶活性的影响

Q785; 人工设计并合成2条等电点分别为12.01和3.18的短肽PF1、PF2,基因全长均为309 bp.以PF1-F、PF1-R,PF2-F和PF2-R为引物,扩增至pET-30a(+)载体中进行克隆表达.经Ni-NTA分离纯化得到纯蛋白.十二烷基硫酸钠-聚丙烯酰胺凝胶电泳与Western Blot分析表明:表达出的目的蛋白与预期估算大小一致.纯化后的短肽加入葡萄糖氧化酶(glucose oxidase,GOD)催化作用体系中,表明纯化后的短肽分别使GOD活力提高8.34％和降低6.88％.因此可以说明,人工设计的不同电短肽PF1、PF2可以促进或抑制GOD的催化作用,进一步拓宽GOD在...

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Published in食品科学 Vol. 43; no. 22; pp. 215 - 220
Main Authors 李传博, 鲁明杰, 张庆芳, 林禹彤, 窦少华
Format Magazine Article
LanguageChinese
Published 大连大学生命健康学院,辽宁省海洋微生物工程技术研究中心,辽宁大连 116622 25.11.2022
Subjects
带电荷短肽
葡萄糖氧化酶
克隆表达
Online AccessGet full text
ISSN1002-6630
DOI10.7506/spkx1002-6630-20211223-271

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Abstract Q785; 人工设计并合成2条等电点分别为12.01和3.18的短肽PF1、PF2,基因全长均为309 bp.以PF1-F、PF1-R,PF2-F和PF2-R为引物,扩增至pET-30a(+)载体中进行克隆表达.经Ni-NTA分离纯化得到纯蛋白.十二烷基硫酸钠-聚丙烯酰胺凝胶电泳与Western Blot分析表明:表达出的目的蛋白与预期估算大小一致.纯化后的短肽加入葡萄糖氧化酶(glucose oxidase,GOD)催化作用体系中,表明纯化后的短肽分别使GOD活力提高8.34％和降低6.88％.因此可以说明,人工设计的不同电短肽PF1、PF2可以促进或抑制GOD的催化作用,进一步拓宽GOD在食品发酵和食品保鲜方面的应用范围.
AbstractList Q785; 人工设计并合成2条等电点分别为12.01和3.18的短肽PF1、PF2,基因全长均为309 bp.以PF1-F、PF1-R,PF2-F和PF2-R为引物,扩增至pET-30a(+)载体中进行克隆表达.经Ni-NTA分离纯化得到纯蛋白.十二烷基硫酸钠-聚丙烯酰胺凝胶电泳与Western Blot分析表明:表达出的目的蛋白与预期估算大小一致.纯化后的短肽加入葡萄糖氧化酶(glucose oxidase,GOD)催化作用体系中,表明纯化后的短肽分别使GOD活力提高8.34％和降低6.88％.因此可以说明,人工设计的不同电短肽PF1、PF2可以促进或抑制GOD的催化作用,进一步拓宽GOD在食品发酵和食品保鲜方面的应用范围.
Author 林禹彤
鲁明杰
窦少华
张庆芳
李传博
AuthorAffiliation 大连大学生命健康学院,辽宁省海洋微生物工程技术研究中心,辽宁大连 116622
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DOU Shaohua
LIN Yutong
LU Mingjie
ZHANG Qingfang
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DocumentTitle_FL Cloning and Expression of Artificially Designed Peptides PF1 and PF2 and Their Effect on Glucose Oxidase Activity
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Keywords 带电荷短肽
葡萄糖氧化酶
克隆表达
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Snippet Q785; 人工设计并合成2条等电点分别为12.01和3.18的短肽PF1、PF2,基因全长均为309 bp.以PF1-F、PF1-R,PF2-F和PF2-R为引物,扩增至pET-30a(+)载体中进行克隆表达.经Ni-NTA分离纯化得到纯蛋白.十二烷基硫酸钠-聚丙烯酰胺凝胶电泳与Western...
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StartPage 215
Title 人工设计短肽PF1和PF2的克隆表达及其对葡萄糖氧化酶活性的影响
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