常压室温等离子体诱变与微生物微液滴培养选育谷胱甘肽高产菌株

Q815; 为提升野生毕赤酵母菌株BY-1产谷胱甘肽的能力,通过常压室温等离子体(atmospheric and room temperature plasma,ARTP)诱变技术得到诱变菌株BY-1-26.进一步在摇瓶培养过程中添加1,2,4-三氮唑,提高诱变菌株产量.最后将1,2,4-三氮唑作为筛选因子,利用微生物微液滴培养(microbial microdroplet culture system,MMC)仪对诱变菌株进行适应性进化,获得了 1株高产谷胱甘肽的突变株BY-2-24,并对其遗传稳定性进行研究.结果表明:出发菌株BY-1经过ARTP诱变处理、抗性梯度平板初筛、MMC适应性进化...

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Published in食品科学 Vol. 44; no. 4; pp. 200 - 208
Main Authors 凌思雨, 王洲, 张会敏, 李闯, 刘庆涛, 刘艳, 薛正莲
Format Magazine Article
LanguageChinese
Published 安徽工程大学生物与食品工程学院,安徽省工业微生物分子育种工程实验室,微生物发酵安徽省工程技术研究中心,安徽芜湖 241000 25.02.2023
Subjects
谷胱甘肽
适应性进化
菌种筛选
毕赤酵母
常压室温等离子体诱变
Online AccessGet full text
ISSN1002-6630
DOI10.7506/spkx1002-6630-20220302-040

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Abstract Q815; 为提升野生毕赤酵母菌株BY-1产谷胱甘肽的能力,通过常压室温等离子体(atmospheric and room temperature plasma,ARTP)诱变技术得到诱变菌株BY-1-26.进一步在摇瓶培养过程中添加1,2,4-三氮唑,提高诱变菌株产量.最后将1,2,4-三氮唑作为筛选因子,利用微生物微液滴培养(microbial microdroplet culture system,MMC)仪对诱变菌株进行适应性进化,获得了 1株高产谷胱甘肽的突变株BY-2-24,并对其遗传稳定性进行研究.结果表明:出发菌株BY-1经过ARTP诱变处理、抗性梯度平板初筛、MMC适应性进化、摇瓶复筛等,可以选育高产谷胱甘肽突变株.突变株BY-2-24摇瓶产量达(312.13±2.62)mg/L,较出发菌株提高134.26％,且经过7次传代培养,仍然具有较好的遗传稳定性.同时,生物量提高118.33％,表明诱变菌株的生长能力得到提高.研究表明,ARTP与MMC联合应用作为一种简便高效的微生物诱变方式,可用于定向诱变筛选高性能微生物菌株,为高通量选育目标菌株提供了参考.
AbstractList Q815; 为提升野生毕赤酵母菌株BY-1产谷胱甘肽的能力,通过常压室温等离子体(atmospheric and room temperature plasma,ARTP)诱变技术得到诱变菌株BY-1-26.进一步在摇瓶培养过程中添加1,2,4-三氮唑,提高诱变菌株产量.最后将1,2,4-三氮唑作为筛选因子,利用微生物微液滴培养(microbial microdroplet culture system,MMC)仪对诱变菌株进行适应性进化,获得了 1株高产谷胱甘肽的突变株BY-2-24,并对其遗传稳定性进行研究.结果表明:出发菌株BY-1经过ARTP诱变处理、抗性梯度平板初筛、MMC适应性进化、摇瓶复筛等,可以选育高产谷胱甘肽突变株.突变株BY-2-24摇瓶产量达(312.13±2.62)mg/L,较出发菌株提高134.26％,且经过7次传代培养,仍然具有较好的遗传稳定性.同时,生物量提高118.33％,表明诱变菌株的生长能力得到提高.研究表明,ARTP与MMC联合应用作为一种简便高效的微生物诱变方式,可用于定向诱变筛选高性能微生物菌株,为高通量选育目标菌株提供了参考.
Author 刘艳
凌思雨
王洲
薛正莲
张会敏
李闯
刘庆涛
AuthorAffiliation 安徽工程大学生物与食品工程学院,安徽省工业微生物分子育种工程实验室,微生物发酵安徽省工程技术研究中心,安徽芜湖 241000
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Author_FL LIU Qingtao
LING Siyu
ZHANG Huimin
LIU Yan
WANG Zhou
XUE Zhenglian
LI Chuang
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Keywords 谷胱甘肽
适应性进化
菌种筛选
毕赤酵母
常压室温等离子体诱变
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Publisher 安徽工程大学生物与食品工程学院,安徽省工业微生物分子育种工程实验室,微生物发酵安徽省工程技术研究中心,安徽芜湖 241000
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Title 常压室温等离子体诱变与微生物微液滴培养选育谷胱甘肽高产菌株
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