易门豆豉中芽孢杆菌分离筛选及产酶特性研究

TS201.3; 该研究从云南传统发酵豆制品易门豆豉中分离筛选获得产淀粉酶、蛋白酶、脂肪酶和纤维素酶的芽孢杆菌,利用水解圈法初筛、摇瓶发酵复筛得到产酶菌株,并对产酶较高的菌株进行生长性能分析和分子生物学鉴定.结果表明,从易门豆豉中分离纯化得到64株菌,筛选得到1株产淀粉酶和蛋白酶能力均较好菌株NB-13,其淀粉酶和蛋白酶活力分别为100.64 U/mL和34.30 U/mL;1株产脂肪酶能力较好的菌株ND-21,其脂肪酶活力为39.95 U/mL;2株产纤维素酶能力较好的菌株,菌株ND-38、NB-55,其纤维素酶活力分别为1.65 U/mL和1.72 U/mL;由生长状况结果可知,菌株ND-...

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Published in中国酿造 Vol. 43; no. 3; pp. 102 - 108
Main Authors 陈来凤, 周金萍, 徐巽, 吕明奎, 李文刚, 杨颂, 邓梅, 林秋叶
Format Journal Article
LanguageChinese
Published 云南农业大学食品科学技术学院,云南 昆明 650201 25.03.2024
Subjects
growth performance
豆豉
分离筛选
identification
enzyme production
鉴定
产酶
Douchi
Bacillus
isolation and screening
芽孢杆菌
生长性能
Online AccessGet full text
ISSN0254-5071
DOI10.11882/j.issn.0254-5071.2024.03.016

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Abstract TS201.3; 该研究从云南传统发酵豆制品易门豆豉中分离筛选获得产淀粉酶、蛋白酶、脂肪酶和纤维素酶的芽孢杆菌,利用水解圈法初筛、摇瓶发酵复筛得到产酶菌株,并对产酶较高的菌株进行生长性能分析和分子生物学鉴定.结果表明,从易门豆豉中分离纯化得到64株菌,筛选得到1株产淀粉酶和蛋白酶能力均较好菌株NB-13,其淀粉酶和蛋白酶活力分别为100.64 U/mL和34.30 U/mL;1株产脂肪酶能力较好的菌株ND-21,其脂肪酶活力为39.95 U/mL;2株产纤维素酶能力较好的菌株,菌株ND-38、NB-55,其纤维素酶活力分别为1.65 U/mL和1.72 U/mL;由生长状况结果可知,菌株ND-21和NB-13富集的菌量最多,生长能力最强;菌株NB-13和NB-55分别被鉴定为枯草芽孢杆菌(Bacillus subtilis)和解淀粉芽孢杆菌(Bacillus amyloliquefaciens),菌株ND-21、ND-38被鉴定为地衣芽孢杆菌(Bacillus licheniformis).
AbstractList TS201.3; 该研究从云南传统发酵豆制品易门豆豉中分离筛选获得产淀粉酶、蛋白酶、脂肪酶和纤维素酶的芽孢杆菌,利用水解圈法初筛、摇瓶发酵复筛得到产酶菌株,并对产酶较高的菌株进行生长性能分析和分子生物学鉴定.结果表明,从易门豆豉中分离纯化得到64株菌,筛选得到1株产淀粉酶和蛋白酶能力均较好菌株NB-13,其淀粉酶和蛋白酶活力分别为100.64 U/mL和34.30 U/mL;1株产脂肪酶能力较好的菌株ND-21,其脂肪酶活力为39.95 U/mL;2株产纤维素酶能力较好的菌株,菌株ND-38、NB-55,其纤维素酶活力分别为1.65 U/mL和1.72 U/mL;由生长状况结果可知,菌株ND-21和NB-13富集的菌量最多,生长能力最强;菌株NB-13和NB-55分别被鉴定为枯草芽孢杆菌(Bacillus subtilis)和解淀粉芽孢杆菌(Bacillus amyloliquefaciens),菌株ND-21、ND-38被鉴定为地衣芽孢杆菌(Bacillus licheniformis).
Abstract_FL In this study,the Bacillus producing amylase,protease,lipase and cellulase were isolated and screened from Yimen Douchi,a traditional fermented soybean product in Yunnan Province.The enzyme-producing strains were screened by hydrolyzing ring method and shaking flask fermen-tation.The growth performance analysis and molecular biological identification of the strains with high enzyme production were carried out.The results showed that 64 strains were isolated and purified from Yimen Douchi,and one strain of NB-13 with good ability to produce amylase and protease was screened,its amylase and protease activities were 100.64 U/ml and 34.30 U/ml,respectively.One strain ND-21 had good lipase production ability,and the lipase activity was 39.95 U/ml.Two strains with good cellulase-producing ability,strains ND-38 and NB-55 had cellulase activities of 1.65 U/ml and 1.72 U/ml,respectively.According to the results of growth situation,strains ND-21 and NB-13 had the most enriched bacteria and the strongest growth ability.The strains NB-13 and NB-55 were identified as Bacillus subtilis and Bacillus amyloliquefaciens,respectively,and strains ND-21,ND-38 were identified as Bacillus licheniformis.
Author 李文刚
林秋叶
邓梅
徐巽
陈来凤
杨颂
周金萍
吕明奎
AuthorAffiliation 云南农业大学食品科学技术学院,云南 昆明 650201
AuthorAffiliation_xml – name: 云南农业大学食品科学技术学院,云南 昆明 650201
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XU Xun
CHEN Laifeng
YANG Song
DENG Mei
LI Wengang
ZHOU Jinping
LV Mingkui
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DocumentTitle_FL Isolation,screening and enzyme-producing characteristics of Bacillus from Yimen Douchi
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Keywords growth performance
豆豉
分离筛选
identification
enzyme production
鉴定
产酶
Douchi
Bacillus
isolation and screening
芽孢杆菌
生长性能
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Publisher 云南农业大学食品科学技术学院,云南 昆明 650201
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Title 易门豆豉中芽孢杆菌分离筛选及产酶特性研究
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