"易层"贴敷对膝骨关节炎大鼠滑膜纤维化的改善作用及机制
R965; 目的 探究"易层"贴敷对膝骨关节炎(KOA)大鼠滑膜纤维化(SF)的改善作用及潜在机制.方法 将雄性SD大鼠随机分为假手术组、KOA组和易层组,每组8只.KOA和易层组大鼠以前交叉韧带离断法复制KOA模型.造模14 d后,易层组大鼠于膝关节均匀涂抹"易层"贴敷[三色散(每100 cm2涂抹8 g)和复方三黄油膏(每100 cm2涂抹5 g)],每天贴敷8 h,连续28 d.末次给药后,观察各组大鼠的滑膜炎症程度及纤维化程度并进行Krenn评分,检测其滑膜组织中Ⅰ型胶原蛋白(collagenⅠ)、高迁移率族蛋白B1(HMGB1)、磷酸化核因子κ...
Saved in:
| Published in | 中国药房 Vol. 35; no. 4; pp. 407 - 412 |
|---|---|
| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | Chinese |
| Published |
江苏省中医外用药开发与应用工程研究中心,南京 210023
01.02.2024
南京中医药大学附属医院/江苏省中医院骨伤科,南京 210029%南京中医药大学附属医院/江苏省中医院骨伤科,南京 210029 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1001-0408 |
| DOI | 10.6039/j.issn.1001-0408.2024.04.05 |
Cover
| Abstract | R965; 目的 探究"易层"贴敷对膝骨关节炎(KOA)大鼠滑膜纤维化(SF)的改善作用及潜在机制.方法 将雄性SD大鼠随机分为假手术组、KOA组和易层组,每组8只.KOA和易层组大鼠以前交叉韧带离断法复制KOA模型.造模14 d后,易层组大鼠于膝关节均匀涂抹"易层"贴敷[三色散(每100 cm2涂抹8 g)和复方三黄油膏(每100 cm2涂抹5 g)],每天贴敷8 h,连续28 d.末次给药后,观察各组大鼠的滑膜炎症程度及纤维化程度并进行Krenn评分,检测其滑膜组织中Ⅰ型胶原蛋白(collagenⅠ)、高迁移率族蛋白B1(HMGB1)、磷酸化核因子κB p65(p-NF-κB p65)蛋白的表达,血清中白细胞介素1β(IL-1β)、IL-6、肿瘤坏死因子α(TNF-α)的含量,以及滑膜组织中纤维化及HMGB1/Toll样受体4(TLR4)/NF-κB通路相关蛋白及mRNA的表达.结果 与假手术组比较,KOA组大鼠滑膜衬里细胞大量增生且排列紊乱,炎症细胞浸润和胶原纤维沉积明显;collagenⅠ、HMGB1、p-NF-κB p65阳性表达细胞均明显增加,血清中IL-1β、IL-6、TNF-α含量,滑膜组织中纤维化相关蛋白(转化生长因子β、collagenⅠ、金属蛋白酶组织抑制物1、α-平滑肌肌动蛋白)及mRNA的表达,以及HMGB1、TLR4蛋白及mRNA,p-NF-κB p65蛋白及NF-κB p65 mRNA的表达均显著升高(P＜0.01).与KOA组比较,易层组大鼠滑膜组织的病理改变明显改善,上述定量指标均显著逆转(P＜0.05或P＜0.01).结论"易层"贴敷可显著改善KOA大鼠的SF,其作用可能与抑制HMGB1/TLR4/NF-κB信号通路激活有关. |
|---|---|
| AbstractList | R965; 目的 探究"易层"贴敷对膝骨关节炎(KOA)大鼠滑膜纤维化(SF)的改善作用及潜在机制.方法 将雄性SD大鼠随机分为假手术组、KOA组和易层组,每组8只.KOA和易层组大鼠以前交叉韧带离断法复制KOA模型.造模14 d后,易层组大鼠于膝关节均匀涂抹"易层"贴敷[三色散(每100 cm2涂抹8 g)和复方三黄油膏(每100 cm2涂抹5 g)],每天贴敷8 h,连续28 d.末次给药后,观察各组大鼠的滑膜炎症程度及纤维化程度并进行Krenn评分,检测其滑膜组织中Ⅰ型胶原蛋白(collagenⅠ)、高迁移率族蛋白B1(HMGB1)、磷酸化核因子κB p65(p-NF-κB p65)蛋白的表达,血清中白细胞介素1β(IL-1β)、IL-6、肿瘤坏死因子α(TNF-α)的含量,以及滑膜组织中纤维化及HMGB1/Toll样受体4(TLR4)/NF-κB通路相关蛋白及mRNA的表达.结果 与假手术组比较,KOA组大鼠滑膜衬里细胞大量增生且排列紊乱,炎症细胞浸润和胶原纤维沉积明显;collagenⅠ、HMGB1、p-NF-κB p65阳性表达细胞均明显增加,血清中IL-1β、IL-6、TNF-α含量,滑膜组织中纤维化相关蛋白(转化生长因子β、collagenⅠ、金属蛋白酶组织抑制物1、α-平滑肌肌动蛋白)及mRNA的表达,以及HMGB1、TLR4蛋白及mRNA,p-NF-κB p65蛋白及NF-κB p65 mRNA的表达均显著升高(P＜0.01).与KOA组比较,易层组大鼠滑膜组织的病理改变明显改善,上述定量指标均显著逆转(P＜0.05或P＜0.01).结论"易层"贴敷可显著改善KOA大鼠的SF,其作用可能与抑制HMGB1/TLR4/NF-κB信号通路激活有关. |
| Abstract_FL | OBJECTIVE To investigate the improvement effect and potential mechanism of"Layers adjusting external application"paste on synovial fibrosis(SF)in rats with knee osteoarthritis(KOA).METHODS Male SD rats were randomly divided into sham operation group,KOA group and Layers adjusting external application group,with 8 rats in each group.KOA model was induced by the anterior cruciate ligament disruption method in KOA group and Layers adjusting external application group.Fourteen days after modeling,the Layers adjusting external application group was given"Layers adjusting external application"paste[Sanse powder(8 g for every 100 cm2),Compound sanhuang ointment(5 g for every 100 cm2)]on the knee joint,8 h every day,for 28 d in total.After the last administration,the degree of synovitis and fibrosis in rats was observed,and Krenn scoring was performed in each group.The expressions of collagen Ⅰ,high mobility group protein B1(HMGB1)and phosphorylated nuclear factor-κB p65(p-NF-κB p65)were detected in the synovial membrane;the contents of interleukin-1β(IL-1β),IL-6 and tumor necrosis factor-α(TNF-α)in serum as well as the expressions of fibrosis-related and HMGB1/Toll-like receptor 4(TLR4)/NF-κB signaling pathway-related proteins and mRNA were detected in synovial tissue.RESULTS Compared with the sham operation group,the synovial lining cells in the KOA group showed significant proliferation and disordered arrangement,the inflammatory cell infiltration and collagen fiber deposition were obvious;the positive expressing cells of collagen Ⅰ,HMGB1 and p-NF-κB p65 were increased significantly;the contents of IL-1β,IL-6 and TNF-α in serum,the expressions of fibrosis-related protein(transforming growth factor-β,collagen Ⅰ,tissue inhibitor of metalloproteinase 1,α-smooth muscle actin)and their mRNA as well as the expressions of HMGB1,TLR4 protein and their mRNA,the expressions of p-NF-κB p65 protein and NF-κB p65 mRNA were all increased significantly in synovial tissues of rats(P＜0.01).Compared with the KOA group,the pathological changes in the synovial tissue of rats in Layers adjusting external application group were significantly improved,and the above quantitative indicators were significantly reversed(P＜0.05 or P＜0.01).CONCLUSIONS"Layers adjusting external application"paste could significantly improve SF in KOA rats,the mechanism of which may be associated with the inhibition of the activation of HMGB1/TLR4/NF-κB signaling pathway. |
| Author | 廖太阳 吴鹏 魏义保 杨楠 黄正泉 马振源 王培民 |
| AuthorAffiliation | 南京中医药大学附属医院/江苏省中医院骨伤科,南京 210029%南京中医药大学附属医院/江苏省中医院骨伤科,南京 210029;江苏省中医外用药开发与应用工程研究中心,南京 210023 |
| AuthorAffiliation_xml | – name: 南京中医药大学附属医院/江苏省中医院骨伤科,南京 210029%南京中医药大学附属医院/江苏省中医院骨伤科,南京 210029;江苏省中医外用药开发与应用工程研究中心,南京 210023 |
| Author_FL | HUANG Zhengquan WU Peng MA Zhenyuan YANG Nan WEI Yibao LIAO Taiyang WANG Peimin |
| Author_FL_xml | – sequence: 1 fullname: WEI Yibao – sequence: 2 fullname: MA Zhenyuan – sequence: 3 fullname: LIAO Taiyang – sequence: 4 fullname: YANG Nan – sequence: 5 fullname: WU Peng – sequence: 6 fullname: WANG Peimin – sequence: 7 fullname: HUANG Zhengquan |
| Author_xml | – sequence: 1 fullname: 魏义保 – sequence: 2 fullname: 马振源 – sequence: 3 fullname: 廖太阳 – sequence: 4 fullname: 杨楠 – sequence: 5 fullname: 吴鹏 – sequence: 6 fullname: 王培民 – sequence: 7 fullname: 黄正泉 |
| BookMark | eNo9j81Kw0AcxPdQwbb6EhWPif_dzedRil9Q8aLnss0mxSBbMIjoMdQiRSpiLSIRPBQsXkQMSlt9GzdJ38KIIgwMzGFmfiVUEC3hIrSEQTWA2iu-uh8EQsUAWAENLJUA0VTIpRdQ8T-eR6Ug8AE0bBu4iLYrye21fAkrWRwnN-_yeZx17mdPI3n2mnXDNOzJ4ePs4yGZXmWdKJ0M02ksLwbpXTvpj-Wg_fUZpf2RvOwm0USevy2gOY8dBO7in5fR3vrabnVTqe1sbFVXa0qQ_9AV09YocYGbuk1NwojtATeYZ4FlaATr1DM5djgn2GxYBmtQBmBwnWOXOA4GzaJltPzbe8yEx0Sz7reODkW-WD9tnng_4Dkq6PQb15pr4g |
| ClassificationCodes | R965 |
| ContentType | Journal Article |
| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| Copyright_xml | – notice: Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| DBID | 2B. 4A8 92I 93N PSX TCJ |
| DOI | 10.6039/j.issn.1001-0408.2024.04.05 |
| DatabaseName | Wanfang Data Journals - Hong Kong WANFANG Data Centre Wanfang Data Journals 万方数据期刊 - 香港版 China Online Journals (COJ) China Online Journals (COJ) |
| DatabaseTitleList | |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Pharmacy, Therapeutics, & Pharmacology |
| DocumentTitle_FL | Improvement effect and mechanism of"Layers adjusting external application"paste on synovial fibrosis in rats with knee osteoarthritis |
| EndPage | 412 |
| ExternalDocumentID | zgyf202404005 |
| GrantInformation_xml | – fundername: (国家自然科学基金); (国家自然科学基金); (江苏省研究生科研创新计划项目); (江苏省医学重点学科; (江苏省中医院优秀青年博士培养计划); (江苏省中医院中医膝骨关节炎临床医学创新中心项目) funderid: (国家自然科学基金); (国家自然科学基金); (江苏省研究生科研创新计划项目); (实验室)建设项目); (江苏省中医院优秀青年博士培养计划); (江苏省中医院中医膝骨关节炎临床医学创新中心项目) |
| GroupedDBID | -05 2B. 4A8 5GY 5XA 5XF 92F 92I 93N ABJNI ACGFS ALMA_UNASSIGNED_HOLDINGS CCEZO CIEJG CW9 GROUPED_DOAJ PSX TCJ TGQ U1G U5O |
| ID | FETCH-LOGICAL-s1005-79432e0d759372a29f0d6af808642153f7d1cdd217b86ab3a006d5d1e2cc10483 |
| ISSN | 1001-0408 |
| IngestDate | Thu May 29 04:00:04 EDT 2025 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 4 |
| Keywords | 滑膜炎症 synovial inflammation high mobility group protein B1/Toll-like receptor 4/nuclear factor-κB signaling pathway knee osteoarthritis 高迁移率族蛋白B1/Toll样受体4/核因子κB信号通路 滑膜纤维化 synovial fibrosis 膝骨关节炎 |
| Language | Chinese |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-s1005-79432e0d759372a29f0d6af808642153f7d1cdd217b86ab3a006d5d1e2cc10483 |
| PageCount | 6 |
| ParticipantIDs | wanfang_journals_zgyf202404005 |
| PublicationCentury | 2000 |
| PublicationDate | 2024-02-01 |
| PublicationDateYYYYMMDD | 2024-02-01 |
| PublicationDate_xml | – month: 02 year: 2024 text: 2024-02-01 day: 01 |
| PublicationDecade | 2020 |
| PublicationTitle | 中国药房 |
| PublicationTitle_FL | China Pharmacy |
| PublicationYear | 2024 |
| Publisher | 江苏省中医外用药开发与应用工程研究中心,南京 210023 南京中医药大学附属医院/江苏省中医院骨伤科,南京 210029%南京中医药大学附属医院/江苏省中医院骨伤科,南京 210029 |
| Publisher_xml | – name: 南京中医药大学附属医院/江苏省中医院骨伤科,南京 210029%南京中医药大学附属医院/江苏省中医院骨伤科,南京 210029 – name: 江苏省中医外用药开发与应用工程研究中心,南京 210023 |
| SSID | ssj0041961 ssib051369179 ssib001103784 ssib017477153 |
| Score | 2.343116 |
| Snippet | R965; 目的 探究"易层"贴敷对膝骨关节炎(KOA)大鼠滑膜纤维化(SF)的改善作用及潜在机制.方法 将雄性SD大鼠随机分为假手术组、KOA组和易层组,每组8只.KOA和易层组大鼠以前交叉韧带离断法复制KOA模型.造模14 d后,易层组大鼠于膝关节均匀涂抹"易层"贴敷[三色散(每100 cm2涂抹8... |
| SourceID | wanfang |
| SourceType | Aggregation Database |
| StartPage | 407 |
| Title | "易层"贴敷对膝骨关节炎大鼠滑膜纤维化的改善作用及机制 |
| URI | https://d.wanfangdata.com.cn/periodical/zgyf202404005 |
| Volume | 35 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnR1Na9RAdGgriBfxEz_LUp1e7NZ8TCYzx2Q3SxEqPWyht5JNNvW0gm0P7bHUIkUUsRaRCh4KFi8iFqWt_huzuwV_hO9NZjdZEb8ghOyb9_0y-2bCvBlCbjoJE1HMnXLTYrzMkoiVZexa0K8akI7syIwbWCg8fZdPzbI7c87c0PD3wqql5aXGZLT6y7qS_4kqwCCuWCX7D5HtMwUAPEN84Q4RhvtfxZhaFg04lYJKmwYO9U0qLAUU1Gd4YSvAXWz1atSX2CQAWKWBpJ5HPYFNAnBs1eQhh8DFuwgUFaOei8h-hXoGMvR9Ks0enwoi-x6i4YOvhALDCpUcIRIYZmowJd1BOELgZ1WTQ5NWo4YKBIot8EQIGMKLA2hFKKhXVax8ZIKaVNE6IET8_lplZWIVuQZKuJDqoQbWD6DAVVHEFc0FZEsjR3GUybznDQ-JwOe-naMoj6IRnHqAZRQ_plist_5av_5KhEllTanuKwVdtFmYA-aBPiA4k6q9mXmqb7CDURGG9p3skWeRQyvYgH_hPQDtAOIpufhgaBxwgs8HpIOfhA09UDGHULmqFSJduWXhjrp2IZ-pFXPMEMWEl-0Pozs2K2Qvlh1ArAdCLFvf_nOO5YYtVY5FAZN9AZPoTrVrsJMPLfoLPlcXVhJEwJThDJMTlutmiyr0BxA1eMeS1bxqGqbKgJQPJh3T5lIdl5eNsxjkDvU5pafBSXJDq3f7N8qp4rxWErYWCuPI-hlyWk8AS17Wm8-SodV758j4TLaD_MpEqZ4XRC5OlMZLM_ne8ivnyfRY--Xz9MPaWHd_v_3ic_r-oLvx-vjdXvrwY3dzrbP2JN19e_zlTfvoWXdjp3O42znaTx9vd16tt7cO0u31b193Olt76dPN9s5h-ujTBTJbC-qVqbI-EaW8aOKWwbibo9U0YteBWYUVWjIxYh4mwhBYr-7YiRubURxbptsQPGzYIeTU2InNphVFJh4ecZGMtO63mpdICQhjHvEo5CZnocnCECYrTWnEDCZEthtdJqPaT_P6H29xfiCKV_6EcJWcyrvYNTKy9GC5eR1G8EuNURX4HygWt50 |
| linkProvider | Directory of Open Access Journals |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=%22%E6%98%93%E5%B1%82%22%E8%B4%B4%E6%95%B7%E5%AF%B9%E8%86%9D%E9%AA%A8%E5%85%B3%E8%8A%82%E7%82%8E%E5%A4%A7%E9%BC%A0%E6%BB%91%E8%86%9C%E7%BA%A4%E7%BB%B4%E5%8C%96%E7%9A%84%E6%94%B9%E5%96%84%E4%BD%9C%E7%94%A8%E5%8F%8A%E6%9C%BA%E5%88%B6&rft.jtitle=%E4%B8%AD%E5%9B%BD%E8%8D%AF%E6%88%BF&rft.au=%E9%AD%8F%E4%B9%89%E4%BF%9D&rft.au=%E9%A9%AC%E6%8C%AF%E6%BA%90&rft.au=%E5%BB%96%E5%A4%AA%E9%98%B3&rft.au=%E6%9D%A8%E6%A5%A0&rft.date=2024-02-01&rft.pub=%E6%B1%9F%E8%8B%8F%E7%9C%81%E4%B8%AD%E5%8C%BB%E5%A4%96%E7%94%A8%E8%8D%AF%E5%BC%80%E5%8F%91%E4%B8%8E%E5%BA%94%E7%94%A8%E5%B7%A5%E7%A8%8B%E7%A0%94%E7%A9%B6%E4%B8%AD%E5%BF%83%2C%E5%8D%97%E4%BA%AC+210023&rft.issn=1001-0408&rft.volume=35&rft.issue=4&rft.spage=407&rft.epage=412&rft_id=info:doi/10.6039%2Fj.issn.1001-0408.2024.04.05&rft.externalDocID=zgyf202404005 |
| thumbnail_s | http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.wanfangdata.com.cn%2Fimages%2FPeriodicalImages%2Fzgyf%2Fzgyf.jpg |