一株枯草芽胞杆菌L249的分离鉴定及对烟草赤星病的盆栽防效
S435.72; 为筛选出对烟草赤星病具有生防潜力的拮抗细菌,以链格孢菌(Alteraria alternata)为靶标菌,采用5点取样法在赤星病发生严重地块采集健康烟株根际土壤,通过梯度稀释分离法和平板对峙法筛选得到一株具有生防作用的拮抗菌L249,对链格孢菌抑菌率达72.27％,经菌落形态、生理生化和分子生物学鉴定为枯草芽胞杆菌(Bacillus subtilis).该菌能产生蛋白酶、纤维素酶和硝酸还原酶等生防相关酶类.利用比浊法测定菌株L249的生长速率,结果表明该菌培养4h后进入对数生长期,14 h后进入稳定生长期.盆栽试验结果表明,菌株L249发酵液能有效抑制烟草赤星病的发生,相对防...
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| Published in | 烟草科技 Vol. 56; no. 5; pp. 25 - 32 |
|---|---|
| Main Authors | , , , , , |
| Format | Journal Article |
| Language | Chinese |
| Published |
贵州大学烟草学院,贵阳市花溪区花溪大道南段2708号 550025%贵州大学烟草学院,贵阳市花溪区花溪大道南段2708号 550025
01.05.2023
贵州省烟草品质研究重点实验室,贵阳市花溪区花溪大道南段2708号 550025%贵州大学农学院,贵阳市花溪区花溪大道南段2708号 550025 |
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| Online Access | Get full text |
| ISSN | 1002-0861 |
| DOI | 10.16135/j.issn1002-0861.2022.0715 |
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| Abstract | S435.72; 为筛选出对烟草赤星病具有生防潜力的拮抗细菌,以链格孢菌(Alteraria alternata)为靶标菌,采用5点取样法在赤星病发生严重地块采集健康烟株根际土壤,通过梯度稀释分离法和平板对峙法筛选得到一株具有生防作用的拮抗菌L249,对链格孢菌抑菌率达72.27％,经菌落形态、生理生化和分子生物学鉴定为枯草芽胞杆菌(Bacillus subtilis).该菌能产生蛋白酶、纤维素酶和硝酸还原酶等生防相关酶类.利用比浊法测定菌株L249的生长速率,结果表明该菌培养4h后进入对数生长期,14 h后进入稳定生长期.盆栽试验结果表明,菌株L249发酵液能有效抑制烟草赤星病的发生,相对防效达60.66％,与10％苯醚甲环唑水分散粒剂的相对防效差异不显著.枯草芽胞杆菌L249可有效防治烟草赤星病,具有较好的生防潜力. |
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| AbstractList | S435.72; 为筛选出对烟草赤星病具有生防潜力的拮抗细菌,以链格孢菌(Alteraria alternata)为靶标菌,采用5点取样法在赤星病发生严重地块采集健康烟株根际土壤,通过梯度稀释分离法和平板对峙法筛选得到一株具有生防作用的拮抗菌L249,对链格孢菌抑菌率达72.27％,经菌落形态、生理生化和分子生物学鉴定为枯草芽胞杆菌(Bacillus subtilis).该菌能产生蛋白酶、纤维素酶和硝酸还原酶等生防相关酶类.利用比浊法测定菌株L249的生长速率,结果表明该菌培养4h后进入对数生长期,14 h后进入稳定生长期.盆栽试验结果表明,菌株L249发酵液能有效抑制烟草赤星病的发生,相对防效达60.66％,与10％苯醚甲环唑水分散粒剂的相对防效差异不显著.枯草芽胞杆菌L249可有效防治烟草赤星病,具有较好的生防潜力. |
| Author | 莫维弟 胡珊 周志成 丁海霞 彭剑涛 彭丽娟 |
| AuthorAffiliation | 贵州大学烟草学院,贵阳市花溪区花溪大道南段2708号 550025%贵州大学烟草学院,贵阳市花溪区花溪大道南段2708号 550025;贵州省烟草品质研究重点实验室,贵阳市花溪区花溪大道南段2708号 550025%贵州大学农学院,贵阳市花溪区花溪大道南段2708号 550025 |
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| Author_FL | DING Haixia PENG Jiantao MO Weidi HU Shan ZHOU Zhicheng PENG Lijuan |
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| DocumentTitle_FL | Isolation and identification of Bacillus subtilis strain L249 and its control effect on tobacco brown spot disease in pot experiments |
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| Keywords | 生长速率 枯草芽胞杆菌 防效 烟草赤星病 分离鉴定 |
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| Snippet | S435.72; 为筛选出对烟草赤星病具有生防潜力的拮抗细菌,以链格孢菌(Alteraria... |
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| Title | 一株枯草芽胞杆菌L249的分离鉴定及对烟草赤星病的盆栽防效 |
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