基于SLAF-seq技术的不同类型越橘遗传变异和群体结构研究

S663.9; [目的]利用越橘属栽培品种及野生资源在全基因组范围内筛选差异SNP分子标记,分析其遗传变异,了解不同类型群体间的遗传变异、基因交流、群体结构,为越橘种质创新提供遗传背景依据.[方法]以越橘属9个种的62份种质资源为材料,利用特异性位点扩增片段测序技术(SLAF-seq)获得的基因组SNP标记比对到品种Draper参考基因组上,进行供试材料遗传研究.[结果]测序获得169.87 Mb reads数据,平均Q30值94.99％,包含172513个多态性SLAF标签,筛选到高质量SNP标记4133595个,均匀分布在越橘12条染色体上.基于SNP标记的系统进化树准确反映了供试野生、北...

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Published in果树学报 Vol. 40; no. 8; pp. 1534 - 1545
Main Authors 刘有春, 李嘉琦, 魏鑫, 杨艳敏, 张舵, 王兴东, 孙斌, 杨玉春, 王升, 高树清, 王宏光, 徐艺格, 袁兴福, 刘成
Format Journal Article
LanguageChinese
Published 辽宁省果树科学研究所,辽宁营口 115009%辽宁省农业科学院,沈阳 110161 2023
Subjects
SLAF-seq技术
SNP
Genetic structure
Blueberry
SLAF-seq
遗传群体结构
越橘
Online AccessGet full text
ISSN1009-9980
DOI10.13925/j.cnki.gsxb.20230008

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Abstract S663.9; [目的]利用越橘属栽培品种及野生资源在全基因组范围内筛选差异SNP分子标记,分析其遗传变异,了解不同类型群体间的遗传变异、基因交流、群体结构,为越橘种质创新提供遗传背景依据.[方法]以越橘属9个种的62份种质资源为材料,利用特异性位点扩增片段测序技术(SLAF-seq)获得的基因组SNP标记比对到品种Draper参考基因组上,进行供试材料遗传研究.[结果]测序获得169.87 Mb reads数据,平均Q30值94.99％,包含172513个多态性SLAF标签,筛选到高质量SNP标记4133595个,均匀分布在越橘12条染色体上.基于SNP标记的系统进化树准确反映了供试野生、北高丛和南高丛类群遗传差异以及品种间系谱遗传关系,反映出野生类型与栽培品种遗传差异显著,供试野生种具有独立的遗传背景且种间无基因交流发生,但野生种到栽培种存在单向的基因流;栽培品种类群存在明显的亚群结构,亚群间存在普遍的基因交流;半高丛品种北陆和南高丛品种军号与北高丛类型紧密聚类,矮丛品种美登、半高丛品种北村、黑珍珠遗传背景倾向于野生种.[结论]栽培品种近亲杂交和骨干亲本高频率使用导致品种间基因交流频繁、同质性高、遗传基础狭窄,野生种具有独立的遗传背景,但存在野生种到栽培种的单向基因流,应加大野生资源发掘利用以拓宽越橘遗传基础.
AbstractList S663.9; [目的]利用越橘属栽培品种及野生资源在全基因组范围内筛选差异SNP分子标记,分析其遗传变异,了解不同类型群体间的遗传变异、基因交流、群体结构,为越橘种质创新提供遗传背景依据.[方法]以越橘属9个种的62份种质资源为材料,利用特异性位点扩增片段测序技术(SLAF-seq)获得的基因组SNP标记比对到品种Draper参考基因组上,进行供试材料遗传研究.[结果]测序获得169.87 Mb reads数据,平均Q30值94.99％,包含172513个多态性SLAF标签,筛选到高质量SNP标记4133595个,均匀分布在越橘12条染色体上.基于SNP标记的系统进化树准确反映了供试野生、北高丛和南高丛类群遗传差异以及品种间系谱遗传关系,反映出野生类型与栽培品种遗传差异显著,供试野生种具有独立的遗传背景且种间无基因交流发生,但野生种到栽培种存在单向的基因流;栽培品种类群存在明显的亚群结构,亚群间存在普遍的基因交流;半高丛品种北陆和南高丛品种军号与北高丛类型紧密聚类,矮丛品种美登、半高丛品种北村、黑珍珠遗传背景倾向于野生种.[结论]栽培品种近亲杂交和骨干亲本高频率使用导致品种间基因交流频繁、同质性高、遗传基础狭窄,野生种具有独立的遗传背景,但存在野生种到栽培种的单向基因流,应加大野生资源发掘利用以拓宽越橘遗传基础.
Author 徐艺格
李嘉琦
王兴东
魏鑫
杨艳敏
王升
刘成
刘有春
杨玉春
袁兴福
孙斌
王宏光
张舵
高树清
AuthorAffiliation 辽宁省果树科学研究所,辽宁营口 115009%辽宁省农业科学院,沈阳 110161
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Author_FL ZHANG Duo
YANG Yuchun
WANG Sheng
LIU Cheng
LI Jiaqi
WEI Xin
WANG Xingdong
XU Yige
YANG Yanmin
SUN Bin
WANG Hongguang
GAO Shuqing
LIU Youchun
YUAN Xingfu
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DocumentTitle_FL Genetic variation and structure analysis of different type blueberries (Vaccinium spp.) based on SLAF-seq technology
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Keywords SLAF-seq技术
SNP
Genetic structure
Blueberry
SLAF-seq
遗传群体结构
越橘
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Title 基于SLAF-seq技术的不同类型越橘遗传变异和群体结构研究
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