基于干细胞因子/C-kit信号通路探讨鼠李糖乳酪杆菌Glory LG12的润肠通便作用
TS201.4; 目的:基于干细胞因子(stem cell factor,SCF)/C-kit信号通路探讨鼠李糖乳酪杆菌Glory LG12的润肠通便作用.方法:雄性ICR小鼠随机分为空白组、模型组以及鼠李糖乳酪杆菌Glory LG12低剂量(1.5×106 CFU/只)、中剂量(1.5×107 CFU/只)和高剂量(1.5×108 CFU/只)组,各组小鼠分别连续灌胃相应的药物15d后,灌胃盐酸洛哌丁胺混悬液(4 mg/kg)建立便秘模型,测定各组小鼠的体质量、首粒黑便排出时间、5h内黑便排出数量及质量、小肠推进率、血清中胃肠调节肽和炎性细胞因子水平及小鼠结肠组织SCF、C-kit转录水平,...
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| Published in | 食品科学 Vol. 46; no. 3; pp. 20 - 26 |
|---|---|
| Main Authors | , , |
| Format | Magazine Article |
| Language | Chinese |
| Published |
东北农业大学食品学院,黑龙江 哈尔滨 150006
15.02.2025
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1002-6630 |
| DOI | 10.7506/spkx1002-6630-20240710-109 |
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| Abstract | TS201.4; 目的:基于干细胞因子(stem cell factor,SCF)/C-kit信号通路探讨鼠李糖乳酪杆菌Glory LG12的润肠通便作用.方法:雄性ICR小鼠随机分为空白组、模型组以及鼠李糖乳酪杆菌Glory LG12低剂量(1.5×106 CFU/只)、中剂量(1.5×107 CFU/只)和高剂量(1.5×108 CFU/只)组,各组小鼠分别连续灌胃相应的药物15d后,灌胃盐酸洛哌丁胺混悬液(4 mg/kg)建立便秘模型,测定各组小鼠的体质量、首粒黑便排出时间、5h内黑便排出数量及质量、小肠推进率、血清中胃肠调节肽和炎性细胞因子水平及小鼠结肠组织SCF、C-kit转录水平,并观察结肠病理结构.结果:与模型组相比,中、高剂量的鼠李糖乳酪杆菌Glory LG12均能缩短小鼠的首粒黑便排出时间(P＜0.001),增加小鼠5h内排黑便的粒数和质量(P＜0.01、P＜0.001),提高小肠内的墨汁推进率(P＜0.001);升高小鼠血清中胃动素、胃泌素、P物质和白细胞介素(interleukin,IL)10的质量浓度,降低内皮素1、生长抑素、血管活性肠肽和IL-6的质量浓度;增加结肠组织中SCF和C-kit mRNA相对表达量(P＜0.01、P＜0.001);修复受损结肠屏障.结论:鼠李糖乳酪杆菌Glory LG12能够通过调节SCF/C-kit通路,进而影响胃肠调节肽和炎性细胞因子的释放,发挥其促进胃肠蠕动的功能,改善便秘症状. |
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| AbstractList | TS201.4; 目的:基于干细胞因子(stem cell factor,SCF)/C-kit信号通路探讨鼠李糖乳酪杆菌Glory LG12的润肠通便作用.方法:雄性ICR小鼠随机分为空白组、模型组以及鼠李糖乳酪杆菌Glory LG12低剂量(1.5×106 CFU/只)、中剂量(1.5×107 CFU/只)和高剂量(1.5×108 CFU/只)组,各组小鼠分别连续灌胃相应的药物15d后,灌胃盐酸洛哌丁胺混悬液(4 mg/kg)建立便秘模型,测定各组小鼠的体质量、首粒黑便排出时间、5h内黑便排出数量及质量、小肠推进率、血清中胃肠调节肽和炎性细胞因子水平及小鼠结肠组织SCF、C-kit转录水平,并观察结肠病理结构.结果:与模型组相比,中、高剂量的鼠李糖乳酪杆菌Glory LG12均能缩短小鼠的首粒黑便排出时间(P＜0.001),增加小鼠5h内排黑便的粒数和质量(P＜0.01、P＜0.001),提高小肠内的墨汁推进率(P＜0.001);升高小鼠血清中胃动素、胃泌素、P物质和白细胞介素(interleukin,IL)10的质量浓度,降低内皮素1、生长抑素、血管活性肠肽和IL-6的质量浓度;增加结肠组织中SCF和C-kit mRNA相对表达量(P＜0.01、P＜0.001);修复受损结肠屏障.结论:鼠李糖乳酪杆菌Glory LG12能够通过调节SCF/C-kit通路,进而影响胃肠调节肽和炎性细胞因子的释放,发挥其促进胃肠蠕动的功能,改善便秘症状. |
| Abstract_FL | Purpose:To explore the laxative effect of Lacticaseibacillus rhamnosus Glory LG12 from the perspective of the stem cell factor(SCF)/C-kit signaling pathway.Methods:Male ICR mice were randomly divided into five groups:control,model,low-(1.5×106 CFU/mouse),medium-(1.5×107 CFU/mouse),and high-(1.5×108 CFU/mouse)dose L.rhamnosus Glory LG12.After 15 continuous days of gavage,all mice were further gavaged with loperamide hydrochloride suspension(4 mg/kg)to establish a mouse model of constipation.Body mass,the time taken until the first black stool excretion,the number and mass of black stool within 5 hours,small intestinal transit rate,serum levels of gastrointestinal regulatory peptides and inflammatory cytokines,colonic SCF and C-kit transcription levels were measured,and colonic pathology was examined.Results:Compared with the model group,medium-and high-dose L.rhamnosus Glory LG12 significantly reduced the time taken until the first black stool excretion(P<0.001),increased the number and mass of black stools within 5 h(P<0.01 and P<0.001,respectively),and improved the ink propulsion rate in the small intestine(P<0.001).In addition,both treatments elevated the serum levels of motilin(MTL),gastrin(Gas),substance P(SP),and interleukin 10(IL-10),reduced the levels of endothelin-1(ET-1),somatostatin(SS),vasoactive intestinal peptide(VIP),and IL-6,up-regulated the relative mRNA expression of SCF and C-kit in colonic tissue was(P<0.01,P<0.001),and restored damaged colonic barrier.Conclusion:L.rhamnosus Glory LG12 promotes gastrointestinal motility by affecting the release of gastrointestinal regulatory peptides and inflammatory cytokines through the modulation of the SCF/C-kit signaling pathway,thereby alleviating constipation symptoms. |
| Author | 项芳琴 李玉星 李柏良 |
| AuthorAffiliation | 东北农业大学食品学院,黑龙江 哈尔滨 150006 |
| AuthorAffiliation_xml | – name: 东北农业大学食品学院,黑龙江 哈尔滨 150006 |
| Author_FL | LI Bailiang XIANG Fangqin LI Yuxing |
| Author_FL_xml | – sequence: 1 fullname: XIANG Fangqin – sequence: 2 fullname: LI Yuxing – sequence: 3 fullname: LI Bailiang |
| Author_xml | – sequence: 1 fullname: 项芳琴 – sequence: 2 fullname: 李玉星 – sequence: 3 fullname: 李柏良 |
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| ClassificationCodes | TS201.4 |
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| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
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| DOI | 10.7506/spkx1002-6630-20240710-109 |
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| DocumentTitle_FL | Laxative Effect of Lacticaseibacillus rhamnosus Glory LG12:from the Perspective of the Stem Cell Factor/C-kit Signaling Pathway |
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| Keywords | Lacticaseibacillus rhamnosus Glory LG12 constipation 鼠李糖乳酪杆菌Glory LG12 gastrointestinal regulatory peptides 便秘 胃肠调节肽 干细胞因子/C-kit信号通路 炎性细胞因子 laxative effect 润肠通便效果 inflammatory cytokines stem cell factor/C-kit signaling pathway |
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| Snippet | TS201.4; 目的:基于干细胞因子(stem cell factor,SCF)/C-kit信号通路探讨鼠李糖乳酪杆菌Glory LG12的润肠通便作用.方法:雄性ICR小鼠随机分为空白组、模型组以及鼠李糖乳酪杆菌Glory LG12低剂量(1.5×106 CFU/只)、中剂量(1.5×107... |
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| Title | 基于干细胞因子/C-kit信号通路探讨鼠李糖乳酪杆菌Glory LG12的润肠通便作用 |
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