超声萃取雾化碳纤维离子化质谱快速区分当归和欧当归
O657.63; 由于当归与欧当归外形相似,常被不法商贩混淆使用.本研究采用超声萃取雾化碳纤维离子化质谱(UENRT/CFI-MS)法研究当归和欧当归的成分差异,以快速区分二者.以甲醇作为提取溶剂,将药材切片样品置于超声雾化片上,利用超声雾化片的高频振荡实现对样品的在线萃取、过滤和雾化.雾化后的中药样品小液滴在施加高压的碳纤维附近被电离,随后对 2种药材的质谱图进行比较分析,寻找差异成分.结果表明,在欧当归的质谱图中识别到m/z 496特征峰,而在当归中未检测到该峰,这一结果为区分 2种药材提供了依据.该方法无需繁琐复杂的样品前处理过程,具有分析速度快、操作简单、绿色环保、灵敏度高等优点,能够...
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| Published in | 质谱学报 Vol. 45; no. 6; pp. 748 - 754 |
|---|---|
| Main Authors | , , , |
| Format | Journal Article |
| Language | Chinese |
| Published |
上海中医药大学交叉科学研究院,上海 201203
01.11.2024
中国科学院上海有机化学研究所,上海有机质谱中心,上海 200032%甘肃省药品检验研究院,甘肃兰州 730030%上海中医药大学交叉科学研究院,上海 201203%中国科学院上海有机化学研究所,上海有机质谱中心,上海 200032 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1004-2997 |
| DOI | 10.7538/zpxb.2024.0108 |
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| Abstract | O657.63; 由于当归与欧当归外形相似,常被不法商贩混淆使用.本研究采用超声萃取雾化碳纤维离子化质谱(UENRT/CFI-MS)法研究当归和欧当归的成分差异,以快速区分二者.以甲醇作为提取溶剂,将药材切片样品置于超声雾化片上,利用超声雾化片的高频振荡实现对样品的在线萃取、过滤和雾化.雾化后的中药样品小液滴在施加高压的碳纤维附近被电离,随后对 2种药材的质谱图进行比较分析,寻找差异成分.结果表明,在欧当归的质谱图中识别到m/z 496特征峰,而在当归中未检测到该峰,这一结果为区分 2种药材提供了依据.该方法无需繁琐复杂的样品前处理过程,具有分析速度快、操作简单、绿色环保、灵敏度高等优点,能够有效快速区分当归及其伪品欧当归. |
|---|---|
| AbstractList | O657.63; 由于当归与欧当归外形相似,常被不法商贩混淆使用.本研究采用超声萃取雾化碳纤维离子化质谱(UENRT/CFI-MS)法研究当归和欧当归的成分差异,以快速区分二者.以甲醇作为提取溶剂,将药材切片样品置于超声雾化片上,利用超声雾化片的高频振荡实现对样品的在线萃取、过滤和雾化.雾化后的中药样品小液滴在施加高压的碳纤维附近被电离,随后对 2种药材的质谱图进行比较分析,寻找差异成分.结果表明,在欧当归的质谱图中识别到m/z 496特征峰,而在当归中未检测到该峰,这一结果为区分 2种药材提供了依据.该方法无需繁琐复杂的样品前处理过程,具有分析速度快、操作简单、绿色环保、灵敏度高等优点,能够有效快速区分当归及其伪品欧当归. |
| Abstract_FL | The rapid and accurate differentiation of Angelica sinensis and Levisticum officinale isa significant challenge in the quality control of traditional Chinese medicine.Angelica sinensis and Levisticum officinale are different genera of the same family.Due to their similar appearances and partially shared chemical components,unscrupulous traders often sell counterfeit Levisticum officinale as Angelica sinensis to make exorbitant profits.In this study,a fast and easy method of ultrasonic extraction and nebulization in real-time/carbon fiber ionization mass spectrometry(UENRT/CFI-MS)was introduced for the swift discrimination of these two easily confused traditional Chinese medicines.UENRT/CFI-MS primarily consists of an online extraction and nebulization unit,an ionization unit.The online extraction and nebulization unit employ an ultrasonic nebulizer chip for real-time sample extraction and nebulization.The ionization unit comprises a 10 mm long carbon fiber rod nested in a metal tube,achieving ionization through the application of a 3 kV high voltage,and the auxiliary solvent(methanol)is continuously supplied to the carbon fiber at a rate of 5 μL/min.Slices of Angelica sinensis and Levisticum officinale from different origins with similar size,appearance,and quality(diameter less than 16 mm,thickness not exceeding 0.5 cm)were selected and placed directly on the nebulizer chip.For each analysis,200 μL methanol was continuously added using a pipette.When the nebulizer was activated,the lower end of the nebulizer chip generated a continuous spray of tiny droplets towards the carbon fiber tip for several seconds,which were then ionized before entering the mass spectrometer for detection.This device integrates online ultrasonic extraction,nebulization,and carbon fiber ionization technologies,enabling real-time analysis of complex samples without cumbersome pretreatment.By applying this technique,a characteristic ion peak of m/z 496 is uniquely identified in Levisticum officinale,serving as a distinctive marker for differentiation of Angelica sinensis and Levisticum officinale.Subsequently,comparative analysis was conducted using sample extracts on electrospray ionization mass spectrometry(ESI-MS)and carbon fiber ionization mass spectrometry(CFI-MS).These experimental results showed that CFI-MS exhibits superior selectivity and efficiency in detecting the m/z 496 characteristic peak,which is not observable in ESI-MS analysis.This finding highlights the unique ability of carbon fiber ionization to selectively detect certain compounds.The UENRT/CFI-MS technique was applied for the direct analysis of Angelica sinensis and Levisticum officinale,and can obtain the analysis results in just a few seconds.A distinctive ion of m/z 496 is detected in Levisticum officinale,facilitating swift discrimination between Angelica sinensis and Levisticum officinale.However,further research is needed to elucidate the structure of the m/z 496 characteristic peak.Additionally,the method's applicability to a wider range of herbal medicines and its performance in more complex matrices should be investigated.This method is expected to play a role in traditional Chinese medicine identification. |
| Author | 郭寅龙 苏越 吕海山 张明童 |
| AuthorAffiliation | 上海中医药大学交叉科学研究院,上海 201203;中国科学院上海有机化学研究所,上海有机质谱中心,上海 200032%甘肃省药品检验研究院,甘肃兰州 730030%上海中医药大学交叉科学研究院,上海 201203%中国科学院上海有机化学研究所,上海有机质谱中心,上海 200032 |
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| Author_FL | LYU Hai-shan SU Yue ZHANG Ming-tong GUO Yin-long |
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| Keywords | direct analysis ultrasonic extraction and nebulization in real-time 直接分析 超声萃取雾化 Angelica sinensis ambient ionization 当归 敞开式离子化 欧当归 质谱 Levisticum officinale mass spectrometry 碳纤维离子化 carbon fiber ionization mass spectrometry |
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| Title | 超声萃取雾化碳纤维离子化质谱快速区分当归和欧当归 |
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