萨能奶山羊初乳和常乳的代谢组学分析

TS252.1; 以萨能奶山羊初乳和常乳为研究对象,采用超高效液相色谱-四极杆静电场轨道阱质谱的非靶向代谢组学方法,探究不同泌乳期萨能奶山羊乳中代谢物的差异以及相关代谢通路的变化.结果表明:初乳中共有118个代谢物与常乳存在差异,其中有62个代谢物相对含量高于常乳,56个代谢物相对含量低于常乳,这些差异性代谢物主要为脂质类、氨基酸类、核苷类等.在代谢通路分析中筛选出与这些差异性代谢物最高的9条关键通路,它们共同调节萨能奶山羊的泌乳过程,其中柠檬酸循环可作为连接其他代谢通路的桥梁.这些代谢通路的命中差异性代谢物数量为12个,初乳中相对含量高的差异性代谢物为牛磺酸、亚牛磺酸、牛磺胆酸、L-苯丙氨酸...

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Published in食品科学 Vol. 44; no. 24; pp. 245 - 252
Main Authors 康佳欣, 李萌, 廖敏和, 商佳琦, 任皓威, 卢玺丞, 刘宁
Format Magazine Article
LanguageChinese
Published 东北农业大学食品学院,乳品科学教育部重点实验室,黑龙江哈尔滨 150030%东北农业大学食品学院,乳品科学教育部重点实验室,黑龙江哈尔滨 150030%东北农业大学食品学院,乳品科学教育部重点实验室,黑龙江哈尔滨 150030 01.12.2023
齐齐哈尔大学食品与生物工程学院,黑龙江齐齐哈尔 161006
哈尔滨腾凝科技有限公司,黑龙江哈尔滨 150028
Subjects
常乳
差异性代谢物
Saanen goat
differential metabolites
metabolomics
colostrum
mature milk
代谢组学
萨能奶山羊
初乳
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Abstract TS252.1; 以萨能奶山羊初乳和常乳为研究对象,采用超高效液相色谱-四极杆静电场轨道阱质谱的非靶向代谢组学方法,探究不同泌乳期萨能奶山羊乳中代谢物的差异以及相关代谢通路的变化.结果表明:初乳中共有118个代谢物与常乳存在差异,其中有62个代谢物相对含量高于常乳,56个代谢物相对含量低于常乳,这些差异性代谢物主要为脂质类、氨基酸类、核苷类等.在代谢通路分析中筛选出与这些差异性代谢物最高的9条关键通路,它们共同调节萨能奶山羊的泌乳过程,其中柠檬酸循环可作为连接其他代谢通路的桥梁.这些代谢通路的命中差异性代谢物数量为12个,初乳中相对含量高的差异性代谢物为牛磺酸、亚牛磺酸、牛磺胆酸、L-苯丙氨酸、L-酪氨酸、琥珀酸、异柠檬酸、D-麦芽糖、a-乳糖、4-羟基苯基丙酮酸和甘氨酸,常乳中相对含量高的差异性代谢物为N1-甲基-4-吡啶酮-3-羧酰胺,它们可作为萨能奶山羊初乳和常乳中潜在的标志性代谢物.本研究采用代谢组学技术鉴别不同泌乳期萨能奶山羊乳差异性代谢物,为分析不同泌乳期的其他物种原料乳提供参考.
AbstractList TS252.1; 以萨能奶山羊初乳和常乳为研究对象,采用超高效液相色谱-四极杆静电场轨道阱质谱的非靶向代谢组学方法,探究不同泌乳期萨能奶山羊乳中代谢物的差异以及相关代谢通路的变化.结果表明:初乳中共有118个代谢物与常乳存在差异,其中有62个代谢物相对含量高于常乳,56个代谢物相对含量低于常乳,这些差异性代谢物主要为脂质类、氨基酸类、核苷类等.在代谢通路分析中筛选出与这些差异性代谢物最高的9条关键通路,它们共同调节萨能奶山羊的泌乳过程,其中柠檬酸循环可作为连接其他代谢通路的桥梁.这些代谢通路的命中差异性代谢物数量为12个,初乳中相对含量高的差异性代谢物为牛磺酸、亚牛磺酸、牛磺胆酸、L-苯丙氨酸、L-酪氨酸、琥珀酸、异柠檬酸、D-麦芽糖、a-乳糖、4-羟基苯基丙酮酸和甘氨酸,常乳中相对含量高的差异性代谢物为N1-甲基-4-吡啶酮-3-羧酰胺,它们可作为萨能奶山羊初乳和常乳中潜在的标志性代谢物.本研究采用代谢组学技术鉴别不同泌乳期萨能奶山羊乳差异性代谢物,为分析不同泌乳期的其他物种原料乳提供参考.
Abstract_FL The differences in metabolites and related metabolism pathways in colostrum and mature milk from Saanen goats at different lactation stages were explored by untargeted metabolomics based on ultra-high performance liquid chromatography-quadrupole electrostatic field orbitrap mass spectrometry(UPLC-QE-orbitrap-MS).The results showed that a total of 118 differential metabolites were found between colostrum and mature milk,among which 62 had higher relative contents in colostrum than in mature milk and 56 had lower relative contents in colostrum than in mature milk.These metabolites were mainly lipids,amino acids,and nucleosides.Nine key metabolic pathways most associated with these metabolites were selected,which jointly regulated the lactation process of Saanen goats,and the citric acid cycle could act as a bridge connecting other metabolic pathways.The number of differential metabolites involved in these metabolic pathways was 12.The differential metabolites with relatively high contents in colostrum were taurine,hypotaurine,taurocholic acid,L-phenylalanine,L-tyrosine,succinic acid,isocitrate,D-maltose,a-lactose,4-hydroxyphenylpyruvate and glycine.The differential metabolite with relatively high contents in mature milk was N1-methyl-4-pyridone-3-carboxamide.They could be used as potential marker metabolites in the colostrum and mature milk of Saanen goats.Metabolomics technology can also be used for identifying differential metabolites in milk from other dairy species at different lactation stages.
Author 卢玺丞
李萌
廖敏和
商佳琦
刘宁
康佳欣
任皓威
AuthorAffiliation 齐齐哈尔大学食品与生物工程学院,黑龙江齐齐哈尔 161006;东北农业大学食品学院,乳品科学教育部重点实验室,黑龙江哈尔滨 150030%东北农业大学食品学院,乳品科学教育部重点实验室,黑龙江哈尔滨 150030%东北农业大学食品学院,乳品科学教育部重点实验室,黑龙江哈尔滨 150030;哈尔滨腾凝科技有限公司,黑龙江哈尔滨 150028
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Author_FL LU Xicheng
LI Meng
LIU Ning
LIAO Minhe
REN Haowei
KANG Jiaxin
SHANG Jiaqi
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DocumentTitle_FL Metabolomics Analysis of Colostrum and Mature Milk from Saanen Goats
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Keywords 常乳
差异性代谢物
Saanen goat
differential metabolites
metabolomics
colostrum
mature milk
代谢组学
萨能奶山羊
初乳
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PublicationTitle 食品科学
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Publisher 东北农业大学食品学院,乳品科学教育部重点实验室,黑龙江哈尔滨 150030%东北农业大学食品学院,乳品科学教育部重点实验室,黑龙江哈尔滨 150030%东北农业大学食品学院,乳品科学教育部重点实验室,黑龙江哈尔滨 150030
齐齐哈尔大学食品与生物工程学院,黑龙江齐齐哈尔 161006
哈尔滨腾凝科技有限公司,黑龙江哈尔滨 150028
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