Comparative evaluation of osteoblast cells viability/proliferation on chitosan films by metabolic and membrane integrity assays
Regarding the increased interest in chitosan usage as a component of the composites useful for scaffolds in bone tissue engineering, we tested the viability and proliferation of one osteoblast cell line (SaOS2) versus primary human osteoblasts (hOb1.19) on chitosan film surface. The present study in...
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Published in | Annals of the Romanian society for cell biology Vol. 21; no. 2; pp. 75 - 80 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Arad
"Vasile Goldis" Western University Arad, Romania
01.01.2017
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Subjects | |
Online Access | Get full text |
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Summary: | Regarding the increased interest in chitosan usage as a component of the composites useful for scaffolds in bone tissue engineering, we tested the viability and proliferation of one osteoblast cell line (SaOS2) versus primary human osteoblasts (hOb1.19) on chitosan film surface. The present study investigates comparative cell viability/proliferation for two bone cell phenotypes (tumoral –SaOS2 and normal –primary osteoblast hOb 1.19), in contact with chitosan films, using two assays –a metabolic (resazurin-resorufin) and a membrane integrity assay (SytoxGreen). Comparison among the viability detected by the two evaluated assays showed that cell viability on chitosan films was overestimated by the metabolic assay compared to the membrane integrity assay. Thus, at all time points during the experiment, the ratio between viability for the cell-seeded film versus control was higher for CellTiter-Blue assay. Thus, for higher accuracy in evaluating cell viability/proliferation, we do recommend the usage of the Sytox Green viability assay. It is essential to be able to choose the optimal assay to detect cell viability/proliferation on/in a biomaterial used as a scaffold, mainly regarding the use of multiple cell lines in the construct of a cell-seeded scaffold destined to in vivo implantation. |
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ISSN: | 2067-3019 2067-8282 |
DOI: | 10.ANN/RSCB-2017-0003:RSCB |