Comparative evaluation of osteoblast cells viability/proliferation on chitosan films by metabolic and membrane integrity assays

Regarding the increased interest in chitosan usage as a component of the composites useful for scaffolds in bone tissue engineering, we tested the viability and proliferation of one osteoblast cell line (SaOS2) versus primary human osteoblasts (hOb1.19) on chitosan film surface. The present study in...

Full description

Saved in:
Bibliographic Details
Published inAnnals of the Romanian society for cell biology Vol. 21; no. 2; pp. 75 - 80
Main Authors Berea, G, Sirbu, P D, Olinici, D, Cotrutz, C E
Format Journal Article
LanguageEnglish
Published Arad "Vasile Goldis" Western University Arad, Romania 01.01.2017
Subjects
Acids
Biomaterials
Biomedical materials
Bones
Calcium phosphates
Cell adhesion & migration
Cell growth
Cell proliferation
Cellular biology
Chitosan
Collagen
Metabolism
Methods
Mineralization
Osteoblasts
Phenotypes
Resorufin
Stem cells
Studies
Tissue engineering
Online AccessGet full text

Cover

More Information
Summary:Regarding the increased interest in chitosan usage as a component of the composites useful for scaffolds in bone tissue engineering, we tested the viability and proliferation of one osteoblast cell line (SaOS2) versus primary human osteoblasts (hOb1.19) on chitosan film surface. The present study investigates comparative cell viability/proliferation for two bone cell phenotypes (tumoral –SaOS2 and normal –primary osteoblast hOb 1.19), in contact with chitosan films, using two assays –a metabolic (resazurin-resorufin) and a membrane integrity assay (SytoxGreen). Comparison among the viability detected by the two evaluated assays showed that cell viability on chitosan films was overestimated by the metabolic assay compared to the membrane integrity assay. Thus, at all time points during the experiment, the ratio between viability for the cell-seeded film versus control was higher for CellTiter-Blue assay. Thus, for higher accuracy in evaluating cell viability/proliferation, we do recommend the usage of the Sytox Green viability assay. It is essential to be able to choose the optimal assay to detect cell viability/proliferation on/in a biomaterial used as a scaffold, mainly regarding the use of multiple cell lines in the construct of a cell-seeded scaffold destined to in vivo implantation.
ISSN:2067-3019
2067-8282
DOI:10.ANN/RSCB-2017-0003:RSCB