Effect of allogeneic adipose derived mesenchymal stem cells on regulatory T cells in peripheral blood of patients with premature ovarian failure

• Published in: Jie fang jun yi xue za zhi Vol. 43; no. 4; p. 294
• Main Authors: Kai-jing, SONG, Yuan-li, HE, Hui-hua, CAI, Dong-mei, ZHANG, Rui-chun, HUANG, Dong-hua, SUN
• Format: Journal Article
• Language: Chinese
• Published: Beijing People's Military Medical Press 01.01.2018
• Subjects: Flow cytometry; Stem cells; T cell receptors
• ISSN: 0577-7402
• DOI: 10.11855/j.issn.0577-7402.2018.04.05

Objective To investigate the effect of allogeneic adipose derived mesenchymal stem cells (hADSCs) on regulatory T cells (Tregs) in peripheral blood of patients with premature ovarian failure (POF). Methods hADSCs were isolated from adipose tissue using 0.1% type Ⅰ collagenase digestion. Adipogenic, osteogenic differentiation and surface molecular characterization were also performed. Peripheral blood mononuclear cells (PBMCs) of POF patients were isolated by density gradient centrifugation. PBMCs were co-cultured with 1×104, 2×104 and 1×105 hADSCs for 72 hours under the stimulation of phytohemagglutinin (PHA). The proliferation rate of lymphocytes was measured by CCK-8 method, the proportion of CD4+CD25+Foxp3+ Treg cells was measured by flow cytometry. Real-time fluorescent quantitative PCR was used to detect the mRNA level of Foxp3+. Results hADSCs were positive for CD90, CD105 and negative for CD34, CD45, and has adipogenic and osteogenic differentiation ability. The results of CCK-8 showed that hADSCs coul