Unbiased Quantitative Proteomics of Organoid Models of Pancreatic Cancer

Pancreatic ductal adenocarcinoma (PDAC) is a lethal solid malignancy with many patients succumbing to the disease within 6 months of diagnosis. The mechanisms that underlie PDAC initiation and progression are poorly understood. Current treatment options are primarily limited to chemotherapy, which i...

Full description

Saved in:
Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 2823; p. 77
Main Authors Low, Ronnie Ren Jie, Fung, Ka Yee, Dagley, Laura F, Yousef, Jumana, Emery-Corbin, Samantha J, Putoczki, Tracy L
Format Journal Article
LanguageEnglish
Published United States 2024
Subjects
Online AccessGet more information

Cover

Loading…
Abstract Pancreatic ductal adenocarcinoma (PDAC) is a lethal solid malignancy with many patients succumbing to the disease within 6 months of diagnosis. The mechanisms that underlie PDAC initiation and progression are poorly understood. Current treatment options are primarily limited to chemotherapy, which is often provided with palliative intent. Unfortunately, there are no robust biomarkers to guide treatment selection or monitor treatment response. This is concerning given the increasing incidence of this cancer. We and others have generated organoid models to explore the biology underlying PDAC with the goal of identifying new therapeutic targets. Here we provide protocols to generate a preclinical PDAC organoid model and methods to use these to define the proteomic landscape of this cancer.
AbstractList Pancreatic ductal adenocarcinoma (PDAC) is a lethal solid malignancy with many patients succumbing to the disease within 6 months of diagnosis. The mechanisms that underlie PDAC initiation and progression are poorly understood. Current treatment options are primarily limited to chemotherapy, which is often provided with palliative intent. Unfortunately, there are no robust biomarkers to guide treatment selection or monitor treatment response. This is concerning given the increasing incidence of this cancer. We and others have generated organoid models to explore the biology underlying PDAC with the goal of identifying new therapeutic targets. Here we provide protocols to generate a preclinical PDAC organoid model and methods to use these to define the proteomic landscape of this cancer.
Author Yousef, Jumana
Low, Ronnie Ren Jie
Dagley, Laura F
Emery-Corbin, Samantha J
Fung, Ka Yee
Putoczki, Tracy L
Author_xml – sequence: 1
  givenname: Ronnie Ren Jie
  surname: Low
  fullname: Low, Ronnie Ren Jie
  organization: Currently at the DSB Repair Metabolism Laboratory, The Francis Crick Institute, London, UK
– sequence: 2
  givenname: Ka Yee
  surname: Fung
  fullname: Fung, Ka Yee
  organization: Department of Medical Biology, The University of Melbourne, Parkville, VIC, Australia
– sequence: 3
  givenname: Laura F
  surname: Dagley
  fullname: Dagley, Laura F
  organization: Department of Medical Biology, The University of Melbourne, Parkville, VIC, Australia
– sequence: 4
  givenname: Jumana
  surname: Yousef
  fullname: Yousef, Jumana
  organization: Department of Medical Biology, The University of Melbourne, Parkville, VIC, Australia
– sequence: 5
  givenname: Samantha J
  surname: Emery-Corbin
  fullname: Emery-Corbin, Samantha J
  organization: Department of Medical Biology, The University of Melbourne, Parkville, VIC, Australia
– sequence: 6
  givenname: Tracy L
  surname: Putoczki
  fullname: Putoczki, Tracy L
  email: putoczki.t@wehi.edu.au, putoczki.t@wehi.edu.au
  organization: Department of Medical Biology, The University of Melbourne, Parkville, VIC, Australia. putoczki.t@wehi.edu.au
BackLink https://www.ncbi.nlm.nih.gov/pubmed/39052215$$D View this record in MEDLINE/PubMed
BookMark eNo1j91KxDAUhIMo7o8-gSB5geg5J23TXMqiu8LKrrBel6RJpLJNS9oVfHuLP1czDB_DzIKdxy56xm4Q7hBA3WtVChSgsBBSEwmsijM2R52BKID0jC2G4QMgU5KySzaTGnIizOds8xZtYwbv-OvJxLEZzdh8er5P3ei7tqkH3gW-S-8mdo3jL53zx59ob2Kd_ATXfDVZn67YRTDHwV__6ZIdnh4Pq43Y7tbPq4et6HVZiBw8krIoMzDe5VmwVin0JINCBSbo0mmsqbSqzKGwYVqcSbQ5WWUA0dGS3f7W9ifbelf1qWlN-qr-H9E3CjZNNA
ContentType Journal Article
Copyright 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Copyright_xml – notice: 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
DBID CGR
CUY
CVF
ECM
EIF
NPM
DOI 10.1007/978-1-0716-3922-1_6
DatabaseName Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
DatabaseTitleList MEDLINE
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
DeliveryMethod no_fulltext_linktorsrc
Discipline Biology
EISSN 1940-6029
ExternalDocumentID 39052215
Genre Journal Article
GroupedDBID ---
29M
53G
ACGFS
ALMA_UNASSIGNED_HOLDINGS
CGR
CUY
CVF
ECM
EIF
F5P
NPM
P2P
RSU
SPO
UDS
WH7
ZGI
ID FETCH-LOGICAL-p986-50e127b1340aed54fbb771e23f7170af98d91c28b78506bf047431b52b7a011d2
IngestDate Sat Nov 02 12:23:38 EDT 2024
IsPeerReviewed false
IsScholarly true
Keywords Model
Organoid
FACs
Pancreatic cancer
Proteomics
Language English
License 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
LinkModel OpenURL
MergedId FETCHMERGED-LOGICAL-p986-50e127b1340aed54fbb771e23f7170af98d91c28b78506bf047431b52b7a011d2
PMID 39052215
ParticipantIDs pubmed_primary_39052215
PublicationCentury 2000
PublicationDate 2024-00-00
PublicationDateYYYYMMDD 2024-01-01
PublicationDate_xml – year: 2024
  text: 2024-00-00
PublicationDecade 2020
PublicationPlace United States
PublicationPlace_xml – name: United States
PublicationTitle Methods in molecular biology (Clifton, N.J.)
PublicationTitleAlternate Methods Mol Biol
PublicationYear 2024
SSID ssj0047324
Score 2.2941294
Snippet Pancreatic ductal adenocarcinoma (PDAC) is a lethal solid malignancy with many patients succumbing to the disease within 6 months of diagnosis. The mechanisms...
SourceID pubmed
SourceType Index Database
StartPage 77
SubjectTerms Animals
Biomarkers, Tumor - metabolism
Carcinoma, Pancreatic Ductal - metabolism
Carcinoma, Pancreatic Ductal - pathology
Humans
Mice
Organoids - metabolism
Pancreatic Neoplasms - genetics
Pancreatic Neoplasms - metabolism
Pancreatic Neoplasms - pathology
Proteome
Proteomics - methods
Title Unbiased Quantitative Proteomics of Organoid Models of Pancreatic Cancer
URI https://www.ncbi.nlm.nih.gov/pubmed/39052215
Volume 2823
hasFullText
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3NS8MwFA-bouwifn9LD95GR5umTXsUPxhDx5AJehpJm8gOa8fcDnr1H_clWdYyP1AvZSSjtPn9-vpe-n7vIXQe-SkNs0C4MQsgQMGEuxzHqStU0zTOfWKKPd91o_YD6TyGj7XaeyVraTblrfTtS13Jf1CFMcBVqWT_gOzipDAAvwFfOALCcPwVxg85H8JbKFOpmblWi6k8oJ4qvaDExjpLQ4sti2Gmu56ZUsk9QFr7imnzUoE-qXqod7qltM6SHdnWuU1bqsl88pVTq9LqtCo7CbfmM9F9kedD1Q0ib3aGC9rcWKPCmk9iMXrFnu2eOZtNWJlmDEboRUgjG1EJttXNCUwqmxPCGNREyQu8-bXMLS6EeUHFaJo-Lp9seZm-4SuZVeSCK4ddfxBV_w2AjEca3iABdmGjDf15dqnAtp2qozqNVfePrtrwMS9zQsHhXBSrMvWIl66mgdbtGZZCE-2i9DfRxjy2cC4MUbZQTeTbaM10G33dQW1LF6dKF6eki1NIx9LFMXRRQyVdHEOXXdS_ue5ftt15Iw13nMSRG3rCx5T7AfGYyEIiOafUFziQEMt7TCZxlvgpjjlV5Qu5hLsGt5LDs0oZmP8M76GVvMjFAXLSKM1EiGEBYkG8JE2yIPKoJEwKrEKLQ7RvVmAwNsVSBnZtjr6dOUaNkjonaFXC0ylOwdWb8jMNxgcHPE3r
link.rule.ids 780
linkProvider National Library of Medicine
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Unbiased+Quantitative+Proteomics+of+Organoid+Models+of+Pancreatic+Cancer&rft.jtitle=Methods+in+molecular+biology+%28Clifton%2C+N.J.%29&rft.au=Low%2C+Ronnie+Ren+Jie&rft.au=Fung%2C+Ka+Yee&rft.au=Dagley%2C+Laura+F&rft.au=Yousef%2C+Jumana&rft.date=2024-01-01&rft.eissn=1940-6029&rft.volume=2823&rft.spage=77&rft_id=info:doi/10.1007%2F978-1-0716-3922-1_6&rft_id=info%3Apmid%2F39052215&rft_id=info%3Apmid%2F39052215&rft.externalDocID=39052215