pH is regulated differently by glucose in skeletal muscle from fed and starved rats: A study using (31)P-NMR spectroscopy

The aim of this study was to determine whether exogenous glucose metabolism influences the pH in superfused EDL muscle from growing rats fed or starved for 48 h (body weight 55 and 45 g, respectively). Energy state and intracellular pH of muscle were repeatedly monitored by 31P-nuclear magnetic reso...

Full description

Saved in:
Bibliographic Details
Published inThe Journal of nutrition Vol. 128; no. 1; p. 28
Main Authors Meynial-Denis, Dominique, Mignon, Michele, Foucat, Loic, Bielicki, Guy
Format Journal Article
LanguageEnglish
Published Bethesda American Institute of Nutrition 01.01.1998
Subjects
Online AccessGet full text

Cover

Loading…
Abstract The aim of this study was to determine whether exogenous glucose metabolism influences the pH in superfused EDL muscle from growing rats fed or starved for 48 h (body weight 55 and 45 g, respectively). Energy state and intracellular pH of muscle were repeatedly monitored by 31P-nuclear magnetic resonance spectroscopy (31P-NMRS); glycogen and other energy metabolites were assayed enzymatically in muscle extracts at the end of the experiment. In EDL muscles from starved rats superfused with glucose for 4 h, intracellular pH was elevated (7-7.3), lactate concentration low, glycogen repletion very intense and citrate synthase activity high. We conclude that glucose was routed mainly toward both oxidative phosphorylation and glycogen synthesis in EDL muscles after food deprivation of rats. In contrast, the major pathway in muscles from fed rats may be glycolysis because the glycogen pool remained constant throughout the experiment. The additional and minor pH component (in the range of 6.5 to 6.8) seen in muscles from fed rats, even in the presence of exogenous glucose, might be due to impaired glucose utilization because this component appears also in muscles from starved rats superfused without glucose or with a nonmetabolizable analog of glucose. Consequently, direct pH measurement by 31P-NMR may be considered to be a precise criterion for evaluation of differences in metabolic potentialities of muscle studied ex vivo in relation to the nutritional state of rats.
AbstractList The aim of this study was to determine whether exogenous glucose metabolism influences the pH in superfused EDL muscle from growing rats fed or starved for 48 h (body weight 55 and 45 g, respectively). Energy state and intracellular pH of muscle were repeatedly monitored by 31P-nuclear magnetic resonance spectroscopy (31P-NMRS); glycogen and other energy metabolites were assayed enzymatically in muscle extracts at the end of the experiment. In EDL muscles from starved rats superfused with glucose for 4 h, intracellular pH was elevated (7-7.3), lactate concentration low, glycogen repletion very intense and citrate synthase activity high. We conclude that glucose was routed mainly toward both oxidative phosphorylation and glycogen synthesis in EDL muscles after food deprivation of rats. In contrast, the major pathway in muscles from fed rats may be glycolysis because the glycogen pool remained constant throughout the experiment. The additional and minor pH component (in the range of 6.5 to 6.8) seen in muscles from fed rats, even in the presence of exogenous glucose, might be due to impaired glucose utilization because this component appears also in muscles from starved rats superfused without glucose or with a nonmetabolizable analog of glucose. Consequently, direct pH measurement by 31P-NMR may be considered to be a precise criterion for evaluation of differences in metabolic potentialities of muscle studied ex vivo in relation to the nutritional state of rats.
Author Bielicki, Guy
Mignon, Michele
Meynial-Denis, Dominique
Foucat, Loic
Author_xml – sequence: 1
  givenname: Dominique
  surname: Meynial-Denis
  fullname: Meynial-Denis, Dominique
– sequence: 2
  givenname: Michele
  surname: Mignon
  fullname: Mignon, Michele
– sequence: 3
  givenname: Loic
  surname: Foucat
  fullname: Foucat, Loic
– sequence: 4
  givenname: Guy
  surname: Bielicki
  fullname: Bielicki, Guy
BookMark eNotT0tLAzEYDFLBtvofPjzpYSHJPtJ4K0WtUB9I7yWPL2VrurvmIey_d0HnMjOHmWEWZNb1HV6QOasrVjSM0hmZU8p5UbKmuSKLGE-UUlbJ1ZyMwxbaCAGP2auEFmzrHAbskh9Bj3D02fQRoe0gfqHHpDycczQewYX-DG6KqM5CTCr8TDqoFB9gPflsR8ix7Y5wV7L7j-Lt9RPigCaFPpp-GK_JpVM-4s0_L8n-6XG_2Ra79-eXzXpXDLJOhZDM1Vqr6QandLXStTLMaMmtQbSWO8O0MLXgjEvBUGhdWS2oFE4hVk6WS3L7VzuE_jtjTIdTn0M3LR6YFFUlJ5S_hmdcSg
CODEN JONUAI
ContentType Journal Article
Copyright Copyright American Institute of Nutrition Jan 1998
Copyright_xml – notice: Copyright American Institute of Nutrition Jan 1998
DBID K9.
NAPCQ
DatabaseName ProQuest Health & Medical Complete (Alumni)
Nursing & Allied Health Premium
DatabaseTitle ProQuest Health & Medical Complete (Alumni)
Nursing & Allied Health Premium
DatabaseTitleList ProQuest Health & Medical Complete (Alumni)
DeliveryMethod fulltext_linktorsrc
Discipline Diet & Clinical Nutrition
EISSN 1541-6100
ExternalDocumentID 25571776
GroupedDBID ---
-ET
-~X
.55
.GJ
0R~
0SF
18M
29L
2WC
34G
39C
3O-
48X
53G
5GY
5RE
5VS
85S
AABZA
AACZT
AAPQZ
AAVAP
AAWTL
AAXUO
AAYJJ
ABJNI
ABPTD
ABWST
ACGFO
ACGOD
ACIHN
ACNCT
ACUFI
ACUTJ
ADBBV
ADGZP
ADRTK
ADUKH
ADVEK
ADVLN
AEAQA
AENEX
AETBJ
AFFNX
AFFZL
AFOFC
AFRAH
AFRQD
AFXAL
AGINJ
AGQXC
AGUTN
AHMBA
AITUG
AJEEA
AKRWK
ALMA_UNASSIGNED_HOLDINGS
AMRAJ
BAWUL
BAYMD
BCR
BCRHZ
BES
BKOMP
BLC
BTRTY
C1A
CDBKE
DAKXR
DIK
DU5
E3Z
EBS
EJD
ENERS
EX3
F5P
F9R
FDB
FECEO
FLUFQ
FOEOM
FOTVD
FQBLK
FRP
G8K
GAUVT
GJXCC
GX1
H13
HF~
HZ~
IH2
K-O
K9.
KBUDW
KOP
KQ8
KSI
KSN
L7B
MHKGH
MV1
NAPCQ
NOMLY
NOYVH
NVLIB
O9-
OAUYM
ODMLO
OHT
OK1
OPAEJ
OVD
P-O
P2P
PEA
PQQKQ
PRG
R0Z
RHF
RHI
ROL
ROX
SV3
TEORI
TN5
TNT
TR2
TWZ
UCJ
UHB
UKR
UPT
W2D
W8F
WH7
WOQ
WOW
X7M
XOL
XSW
Y6R
YBU
YHG
YKV
YQT
YSK
ZGI
ZHY
ZXP
~KM
ID FETCH-LOGICAL-p95t-791f5bba61020088b5ac1cb92dceedd2fc1b7c57212971e7bb4db7097faee4f93
ISSN 0022-3166
IngestDate Thu Oct 10 18:13:41 EDT 2024
IsPeerReviewed true
IsScholarly true
Issue 1
Language English
LinkModel OpenURL
MergedId FETCHMERGED-LOGICAL-p95t-791f5bba61020088b5ac1cb92dceedd2fc1b7c57212971e7bb4db7097faee4f93
PQID 197449999
PQPubID 34400
ParticipantIDs proquest_journals_197449999
PublicationCentury 1900
PublicationDate 19980101
PublicationDateYYYYMMDD 1998-01-01
PublicationDate_xml – month: 01
  year: 1998
  text: 19980101
  day: 01
PublicationDecade 1990
PublicationPlace Bethesda
PublicationPlace_xml – name: Bethesda
PublicationTitle The Journal of nutrition
PublicationYear 1998
Publisher American Institute of Nutrition
Publisher_xml – name: American Institute of Nutrition
SSID ssj0001498
Score 1.6019382
Snippet The aim of this study was to determine whether exogenous glucose metabolism influences the pH in superfused EDL muscle from growing rats fed or starved for 48...
SourceID proquest
SourceType Aggregation Database
StartPage 28
SubjectTerms Glucose
Metabolism
Muscular system
Rodents
Title pH is regulated differently by glucose in skeletal muscle from fed and starved rats: A study using (31)P-NMR spectroscopy
URI https://www.proquest.com/docview/197449999
Volume 128
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1bi9NAFB669cUX8YrrqpwHEaVEmmQmF9-Ku7W4u1WWLPatZJKZJRjT0iRC_DH-Vs8kkxuKqC8hZCC0c77Mucz5viHkReRZoU95bGA4bxvUjbnheTw0GJduKCQTjlR858u1s7qmHzZsM5n8GHQtlQV_E33_La_kf6yKz9CuiiX7D5btXooP8B7ti1e0MF7_ysb7lTqP_NAcJ4-RY3vaSZFWKqxs29GTbJZ_QfeieI9fyxxf0rBKpKh1WlU54fAN7xEMecNUr0VnZ2VbR7BxufM_GevLq1nNzFQKmLv9aEO4p5ilTW-IFvnvDCqqDCfEOBVZoiN3pWrStHxrVuJN1rfyi7QbWO5UD0pdQNglUV_VF2kSNYduvy-rvnrR0PlG1YtuW2rUGrEe_cYB68B0tGy2XqypianvfD5azS3vF9jqtdnrfV67z7_-uF1eX1xsg7NNMB6tXTzmW5juus4RObJNNiW3FudXn887T4_Zpdep0ZtaaHPoz-sgJbhL7mgDwKKByj0yEdl9cnyaiAJegpaATaH74w9ItV9BkkMHIRhACHgFGkKQZNBCCBoIgYIQIIQAIQQaQqAg9BYWUAMIagDBK9t8XYMHhuB5SILlWfBuZejTOIy9zwolayoZ5yHOt2qZ8TgLIzPivhWrMCu2ZGRyN2IuhkK-awqXcxpzd-67MhSCSt9-RKaIIvGYAI8cGjOMiyKK_kNa3LO5z6h0ucUEje1jctJO41Z_bfnWxMRXZef-kz-OnpDbPcyekmlxKMUzDBsL_lxb7ydhlHpf
link.rule.ids 315,786,790
linkProvider Library Specific Holdings
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=pH+is+regulated+differently+by+glucose+in+skeletal+muscle+from+fed+and+starved+rats%3A+A+study+using+%2831%29P-NMR+spectroscopy&rft.jtitle=The+Journal+of+nutrition&rft.au=Meynial-Denis%2C+Dominique&rft.au=Mignon%2C+Michele&rft.au=Foucat%2C+Loic&rft.au=Bielicki%2C+Guy&rft.date=1998-01-01&rft.pub=American+Institute+of+Nutrition&rft.issn=0022-3166&rft.eissn=1541-6100&rft.volume=128&rft.issue=1&rft.spage=28&rft.externalDBID=NO_FULL_TEXT&rft.externalDocID=25571776
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0022-3166&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0022-3166&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0022-3166&client=summon