Anti-Le a monoclonal antibody SPM 522 recognizes an extended Le a epitope

• Published in: Bioorganic & medicinal chemistry Vol. 56; p. 116628
• Main Authors: Jegatheeswaran, Sinthuja, Guillemineau, Mickael, Giovane, Richard, Borrillo, Louis, Liao, Liang, Kuir, Deng, Auzanneau, France-Isabelle
• Format: Journal Article
• Language: English
• Published: England 15.02.2022
• Subjects: Antibodies, Monoclonal - chemistry; Antibodies, Monoclonal - immunology; Carbohydrate Conformation; Carbohydrate Sequence; Dose-Response Relationship, Drug; Enzyme-Linked Immunosorbent Assay; Epitopes - immunology; Glycoconjugates - chemical synthesis; Glycoconjugates - chemistry; Glycoconjugates - immunology; Humans; Molecular Structure; Structure-Activity Relationship; Lewis A; LewisALewisX; SPM522 epitope; ELISA; Tumor-associated carbohydrate antigens

Insights into the differential binding characteristics of anti-Le and anti-Le Le monoclonal antibodies (mAbs) provide information to develop Le Le -based cancer immunotherapeutics while avoiding anti-Le autoimmune reactions. We characterized the epitope recognized by anti-Le mAb SPM 522. We synthesi...