Neutralization of infectivity of porcine circovirus type 2 (PCV2) by capsid-binding 2'F-RNA aptamers
Porcine circovirus type 2 (PCV2) is the main causative agent of porcine circovirus-associated diseases (PCVD), which is responsible for economic losses in the swine industry. The capsid protein of PCV2 has important role for virus neutralization that blocks viral infection. To develop the therapeuti...
Saved in:
Published in | Antiviral research Vol. 88; no. 1; pp. 19 - 24 |
---|---|
Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Kidlington
Elsevier
01.10.2010
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Porcine circovirus type 2 (PCV2) is the main causative agent of porcine circovirus-associated diseases (PCVD), which is responsible for economic losses in the swine industry. The capsid protein of PCV2 has important role for virus neutralization that blocks viral infection. To develop the therapeutic agents, two 2'F-RNA aptamers that bound to the PCV2 capsid protein with nanomole affinity were isolated from a 2'F-RNA library by the Systematic Evolution of Ligands by EXponential enrichment (SELEX). The binding affinity of aptamers was analyzed by Electrophoretic Mobility shift assay (EMSA) and surface plasmon resonance (SPR) analysis. The RNA aptamers have been shown to exhibit high affinity and specificity to PCV2 capsid protein and to neutralize PCV2 infectivity in PK-15 cells in dose dependent manner. Neutralizing aptamers such as this could be promising candidates in developing efficacious anti-PCV2 drugs as well as therapeutic delivery reagent. |
---|---|
AbstractList | Porcine circovirus type 2 (PCV2) is the main causative agent of porcine circovirus-associated diseases (PCVD), which is responsible for economic losses in the swine industry. The capsid protein of PCV2 has important role for virus neutralization that blocks viral infection. To develop the therapeutic agents, two 2'F-RNA aptamers that bound to the PCV2 capsid protein with nanomole affinity were isolated from a 2'F-RNA library by the Systematic Evolution of Ligands by EXponential enrichment (SELEX). The binding affinity of aptamers was analyzed by Electrophoretic Mobility shift assay (EMSA) and surface plasmon resonance (SPR) analysis. The RNA aptamers have been shown to exhibit high affinity and specificity to PCV2 capsid protein and to neutralize PCV2 infectivity in PK-15 cells in dose dependent manner. Neutralizing aptamers such as this could be promising candidates in developing efficacious anti-PCV2 drugs as well as therapeutic delivery reagent. |
Author | LEE, Garam HAN, Dongun KANG, Kyung-Sun YOON, Sorah SONG, Jae-Young LEE, Yong-Soon |
Author_xml | – sequence: 1 givenname: Sorah surname: YOON fullname: YOON, Sorah organization: Virology Division, National Veterinary Research and Quarantine Service, 480, Anyang 6-dong, Manan-gu, Anyang, 430-757, Korea, Republic of – sequence: 2 givenname: Garam surname: LEE fullname: LEE, Garam organization: Virology Division, National Veterinary Research and Quarantine Service, 480, Anyang 6-dong, Manan-gu, Anyang, 430-757, Korea, Republic of – sequence: 3 givenname: Dongun surname: HAN fullname: HAN, Dongun organization: Animal Care Division, Cheonan Yonam College, 3-1 Suhyang-ri, Seonghwan-eup, Cheonan, 331-709, Korea, Republic of – sequence: 4 givenname: Jae-Young surname: SONG fullname: SONG, Jae-Young organization: Virology Division, National Veterinary Research and Quarantine Service, 480, Anyang 6-dong, Manan-gu, Anyang, 430-757, Korea, Republic of – sequence: 5 givenname: Kyung-Sun surname: KANG fullname: KANG, Kyung-Sun organization: Department of Veterinary Public Health, Seoul National University, San 56-1, Shillim-dong, Gwanak-gu, Seoul, 151-742, Korea, Republic of – sequence: 6 givenname: Yong-Soon surname: LEE fullname: LEE, Yong-Soon organization: Department of Veterinary Public Health, Seoul National University, San 56-1, Shillim-dong, Gwanak-gu, Seoul, 151-742, Korea, Republic of |
BackLink | http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23289084$$DView record in Pascal Francis https://www.ncbi.nlm.nih.gov/pubmed/20615431$$D View this record in MEDLINE/PubMed |
BookMark | eNpF0E1LxDAQBuAgivuhf0FzkdVDaz7aJD0ui6vCsoosXks6TSVLm9amFeqvt4srzmWY4eEdmBk6dbUzCF1TElJCxf0-1K6zX7bVZcjIuCUiJJSdoClVkgUJScQpmo5SBDyO2ATNvN8TQoRM1DmaMCJoHHE6RfnW9N2YYr91Z2uH6wJbVxg4hHfDYWzqFqwzGGwL9Xix97gbGoMZvn1dvbM7nA0YdONtHmTW5dZ9YLZYB2_bJdZNpyvT-gt0VujSm8tjn6Pd-mG3ego2L4_Pq-UmaFRMA8ZUDjSKgXOmqeSGiFyJyEBGIAbI5aGU0kwmIqMAhkdSM06UyjJdSMHnaPEb27T1Z298l1bWgylL7Uzd-1TGMU-UjMgor46yzyqTp01rK90O6d9fRnBzBNqDLotWO7D-33GmEqIi_gM8h3V_ |
CODEN | ARSRDR |
ContentType | Journal Article |
Copyright | 2015 INIST-CNRS Copyright © 2010 Elsevier B.V. All rights reserved. |
Copyright_xml | – notice: 2015 INIST-CNRS – notice: Copyright © 2010 Elsevier B.V. All rights reserved. |
DBID | IQODW CGR CUY CVF ECM EIF NPM 7X8 |
DOI | 10.1016/j.antiviral.2010.06.012 |
DatabaseName | Pascal-Francis Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic |
DatabaseTitleList | MEDLINE - Academic MEDLINE |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Medicine Biology |
EISSN | 1872-9096 |
EndPage | 24 |
ExternalDocumentID | 20615431 23289084 |
Genre | Research Support, Non-U.S. Gov't Journal Article |
GroupedDBID | --- --K --M .GJ .~1 0R~ 1B1 1RT 1~. 1~5 23M 4.4 457 4G. 53G 5GY 5VS 7-5 71M 8P~ 9JM AAAJQ AACTN AAEDT AAEDW AAIAV AAIKJ AAKOC AALRI AAOAW AAQFI AAQXK AARKO AATCM AAXUO ABBQC ABFNM ABLVK ABMAC ABMZM ABPIF ABPTK ABXDB ABYKQ ABZDS ACDAQ ACGFS ACIUM ACRLP ADBBV ADEZE ADMUD AEBSH AEKER AENEX AFFNX AFKWA AFTJW AFXIZ AGEKW AGHFR AGUBO AGYEJ AHHHB AIEXJ AIKHN AITUG AJBFU AJOXV AJRQY ALCLG ALMA_UNASSIGNED_HOLDINGS AMFUW AMRAJ ANZVX ASPBG AVWKF AXJTR AZFZN BKOJK BLXMC BNPGV C45 CJTIS CNWQP CS3 EBS EFJIC EJD EO8 EO9 EP2 EP3 F5P FDB FEDTE FGOYB FIRID FNPLU FYGXN G-2 G-Q GBLVA HMG HZ~ IHE IQODW J1W KOM LCYCR LUGTX M41 MO0 N9A O-L O9- OAUVE OGGZJ OVD OZT P-8 P-9 P2P PC. Q38 R2- RIG ROL RPZ SCC SDF SDG SDP SES SEW SIN SPCBC SSH SSI SSP SSZ T5K TEORI WUQ ZGI ZXP ~G- AAXKI ABJNI AFJKZ AKRWK CGR CUY CVF ECM EIF HVGLF NPM 7X8 |
ID | FETCH-LOGICAL-p851-228dc145c332a173e06d864ecb0c5ccd7777788a2796b1cce347a23088bbaf763 |
ISSN | 0166-3542 |
IngestDate | Sat Aug 17 02:16:43 EDT 2024 Sat Sep 28 07:51:03 EDT 2024 Sun Oct 22 16:08:00 EDT 2023 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 1 |
Keywords | RNA Porcine circovirus PCV2 Virus Therapeutic agents 2'F RNA aptamer Circovirus Infectivity Antiviral Aptamer Circoviridae Infected cell Capsid Mechanism of action Veterinary Neutralization |
Language | English |
License | CC BY 4.0 Copyright © 2010 Elsevier B.V. All rights reserved. |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-p851-228dc145c332a173e06d864ecb0c5ccd7777788a2796b1cce347a23088bbaf763 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
PMID | 20615431 |
PQID | 755398740 |
PQPubID | 23479 |
PageCount | 6 |
ParticipantIDs | proquest_miscellaneous_755398740 pubmed_primary_20615431 pascalfrancis_primary_23289084 |
PublicationCentury | 2000 |
PublicationDate | 2010-Oct |
PublicationDateYYYYMMDD | 2010-10-01 |
PublicationDate_xml | – month: 10 year: 2010 text: 2010-Oct |
PublicationDecade | 2010 |
PublicationPlace | Kidlington |
PublicationPlace_xml | – name: Kidlington – name: Netherlands |
PublicationTitle | Antiviral research |
PublicationTitleAlternate | Antiviral Res |
PublicationYear | 2010 |
Publisher | Elsevier |
Publisher_xml | – name: Elsevier |
SSID | ssj0006798 |
Score | 1.9752576 |
Snippet | Porcine circovirus type 2 (PCV2) is the main causative agent of porcine circovirus-associated diseases (PCVD), which is responsible for economic losses in the... |
SourceID | proquest pubmed pascalfrancis |
SourceType | Aggregation Database Index Database |
StartPage | 19 |
SubjectTerms | Animals Antibiotics. Antiinfectious agents. Antiparasitic agents Antiviral agents Antiviral Agents - pharmacology Aptamers, Nucleotide - metabolism Aptamers, Nucleotide - pharmacology Aptamers, Nucleotide - therapeutic use Biological and medical sciences Capsid - drug effects Capsid Proteins - genetics Capsid Proteins - metabolism Cell Line Circovirus - drug effects Circovirus - genetics Circovirus - physiology Fluorescent Antibody Technique, Indirect Gene Expression Medical sciences Pharmacology. Drug treatments Polymerase Chain Reaction Pyrimidines Recombinant Proteins RNA Sequence Analysis, DNA Swine Virulence - drug effects Virus Replication - drug effects |
Title | Neutralization of infectivity of porcine circovirus type 2 (PCV2) by capsid-binding 2'F-RNA aptamers |
URI | https://www.ncbi.nlm.nih.gov/pubmed/20615431 https://search.proquest.com/docview/755398740 |
Volume | 88 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1ba9swFBZbx8ZextbdsnZFD4NtFAdbvkh-LKVpGW02VnfkzUiyXDqoHZy40D7st-_o4jimLbvkwSQCK4nOp-NPR9_RQegDcHKuAlJ4TMElAs7qCT8Rel7FRIZCMHOI68k0OTqLvsziWZ9CYLJLlmIsb-7MK_kfq0Ib2FVnyf6DZVedQgO8B_vCFSwM17-y8VS1JlBxs-J9Tlt15YQWQK71xvmuvGhkfXXRtIsu5grE8tv-D6KDAkBAJZ_Dr9erZJPjAs104n2f7u3y-ZJfOpH86rDaSn9BYyoCrMXCjOuwe_in9VqY2Ul9DnnDL3t_Z51dXZ23K3SedupgrjzjhNYjElrd4XcRCWW9KKPES31bqrZzs4zdgpP1mc5l2qevTai-5ddtiOHnmHf_0InykrHvVNiDk7SnX_PJ2fFxnh3MsofoEaFprOWe41-9_EfvPg30fnd2raWyfAGzpbRlTu5fhxg-kj1Hz9xCAu9ZVLxAD1S1iR7b0qLXm-jJiRNNvETFECa4LvEaTPRHBxPcwwRrmGCCP2mQfMbiGg8hgslHAxDcAeQVyiYH2f6R54preHMg2R4hrJBBFMswJDygofKTgiWRksKXsZQF1S_GOAxdIgIpVRhRDstVxoTgJTyUXqONqq7UW4RhOhcBFdCbCoASRaII0hImfFxS6ImnI7QzGMN8bs9RyYHMs9Rn0QjhblBzcG56x4pXqm4XOY3jMNVFI0fojR3s_mbNxYH9vvvzzVvoaQ_TbbSxbFr1HqjkUuwYTPwGZKZ2EA |
link.rule.ids | 315,786,790,27955,27956 |
linkProvider | Elsevier |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Neutralization+of+infectivity+of+porcine+circovirus+type+2+%28PCV2%29+by+capsid-binding+2%27F-RNA+aptamers&rft.jtitle=Antiviral+research&rft.au=Yoon%2C+Sorah&rft.au=Lee%2C+Garam&rft.au=Han%2C+Dongun&rft.au=Song%2C+Jae-Young&rft.date=2010-10-01&rft.eissn=1872-9096&rft.volume=88&rft.issue=1&rft.spage=19&rft.epage=24&rft_id=info:doi/10.1016%2Fj.antiviral.2010.06.012&rft.externalDBID=NO_FULL_TEXT |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0166-3542&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0166-3542&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0166-3542&client=summon |