Comparative micronucleus quantitation in pre- and post-column fractionated mouse bone marrow by manual and flow methods

• Published in: Mutation research Vol. 302; no. 2; p. 119
• Main Authors: Kirshna, G, Brott, D, Urda, G, McKeel, M, Zandee, J, Theiss, J
• Format: Journal Article
• Language: English
• Published: Netherlands 01.06.1993
• Subjects: Analysis of Variance; Animals; Bone Marrow - ultrastructure; Bone Marrow Cells; Cell Separation; Cyclophosphamide - toxicity; DNA - drug effects; DNA Damage; Dose-Response Relationship, Drug; Flow Cytometry; Male; Mice; Micronucleus Tests - methods; Pilot Projects

In the present study, cellulose-column fractionation methodology which has been used to eliminate nucleated cells in bone marrow was verified for its usefulness in micronucleus analysis and compared to standard smear methodology using cyclophosphamide as the test compound. Also, the possibility of u...