超高效液相色谱-串联质谱法测定人尿中12种典型个人护理品
O658; 研究建立了一种超高效液相色谱-串联质谱法(UPLC-MS/MS)同时测定人尿中12种典型个人护理品(PCPs)的分析方法,其中包括5种对羟基苯甲酸酯类防腐剂(PBs)、5种二苯酮类紫外吸收剂(BPs)和2种抗菌剂.1 Ml尿样中加入β-葡萄糖醛酸酶-乙酸铵缓冲溶液和混合内标工作液置于37℃水浴酶解过夜(≥16 h).样品采用Oasis HLB固相萃取柱富集净化,在乙腈-水流动相体系下采用Acquity BEH C18色谱柱进行色谱分离,负离子电喷雾(ESI-)多反应监测(MRM)模式检测,稳定同位素内标法定量.实验通过优化仪器质谱参数、流动相和色谱柱等色谱条件以及酶解和固相萃取柱等...
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| Published in | Sepu Vol. 41; no. 4; pp. 312 - 322 |
|---|---|
| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | Chinese English |
| Published |
No. 457, Zhongshan Road, Shahekou District, Dalian City, Liaoning Province
中国疾病预防控制中心环境与健康相关产品安全所,中国疾病预防控制中心环境与人群健康重点实验室,北京100021
08.04.2023
Editorial board of Chinese Journal of Chromatography |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1000-8713 1872-2059 |
| DOI | 10.3724/SP.J.1123.2022.05032 |
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| Abstract | O658; 研究建立了一种超高效液相色谱-串联质谱法(UPLC-MS/MS)同时测定人尿中12种典型个人护理品(PCPs)的分析方法,其中包括5种对羟基苯甲酸酯类防腐剂(PBs)、5种二苯酮类紫外吸收剂(BPs)和2种抗菌剂.1 Ml尿样中加入β-葡萄糖醛酸酶-乙酸铵缓冲溶液和混合内标工作液置于37℃水浴酶解过夜(≥16 h).样品采用Oasis HLB固相萃取柱富集净化,在乙腈-水流动相体系下采用Acquity BEH C18色谱柱进行色谱分离,负离子电喷雾(ESI-)多反应监测(MRM)模式检测,稳定同位素内标法定量.实验通过优化仪器质谱参数、流动相和色谱柱等色谱条件以及酶解和固相萃取柱等前处理条件,获得了最佳实验结果.在优化条件下,羟苯甲酯(MeP)和二苯酮-3(BP-3)在4.00～800μg/L,三氯生(TCS)在5.00～200μg/L,其余9种PCPs在1.00～200μg/L范围内线性关系良好,相关系数大于0.999,方法检出限(MDL)为0.06～1.09μg/L,方法定量限(MQL)为0.08～3.63μg/L.12种分析物的加标回收率为89.5％～111.8％,日内精密度为3.7％～8.9％,日间精密度为2.0％～10.6％.采用稳定同位素内标法校正后12种分析物的基质效应为91.9％～110.1％.该方法成功应用于127份实际尿液样品的测定.结果表明,除羟苯苄酯(BzP)和二苯酮-8(BP-8)未检出外,其余10种典型PCPs均可检出,总体检出率为1.7％～99.7％,其中MeP、羟苯乙酯(EtP)和羟苯丙酯(PrP)检出率和浓度水平处于较高水平.该方法简便灵敏,可为人群尿液样本PCPs的生物监测工作提供可靠的技术支持. |
|---|---|
| AbstractList | 研究建立了一种超高效液相色谱-串联质谱法(UPLC-MS/MS)同时测定人尿中12种典型个人护理品(PCPs)的分析方法,其中包括5种对羟基苯甲酸酯类防腐剂(PBs)、5种二苯酮类紫外吸收剂(BPs)和2种抗菌剂。1 mL尿样中加入
β
-葡萄糖醛酸酶-乙酸铵缓冲溶液和混合内标工作液置于37 ℃水浴酶解过夜(≥16 h)。样品采用Oasis HLB固相萃取柱富集净化,在乙腈-水流动相体系下采用Acquity BEH C
18
色谱柱进行色谱分离,负离子电喷雾(ESI
-
)多反应监测(MRM)模式检测,稳定同位素内标法定量。实验通过优化仪器质谱参数、流动相和色谱柱等色谱条件以及酶解和固相萃取柱等前处理条件,获得了最佳实验结果。在优化条件下,羟苯甲酯(MeP)和二苯酮-3(BP-3)在4.00~800 μg/L,三氯生(TCS)在5.00~200 μg/L,其余9种PCPs在1.00~200 μg/L范围内线性关系良好,相关系数大于0.999,方法检出限(MDL)为0.06~1.09 μg/L,方法定量限(MQL)为0.08~3.63 μg/L。12种分析物的加标回收率为89.5%~111.8%,日内精密度为3.7%~8.9%,日间精密度为2.0%~10.6%。采用稳定同位素内标法校正后12种分析物的基质效应为91.9%~110.1%。该方法成功应用于127份实际尿液样品的测定。结果表明,除羟苯苄酯(BzP)和二苯酮-8(BP-8)未检出外,其余10种典型PCPs均可检出,总体检出率为1.7%~99.7%,其中MeP、羟苯乙酯(EtP)和羟苯丙酯(PrP)检出率和浓度水平处于较高水平。该方法简便灵敏,可为人群尿液样本PCPs的生物监测工作提供可靠的技术支持。 O658; 研究建立了一种超高效液相色谱-串联质谱法(UPLC-MS/MS)同时测定人尿中12种典型个人护理品(PCPs)的分析方法,其中包括5种对羟基苯甲酸酯类防腐剂(PBs)、5种二苯酮类紫外吸收剂(BPs)和2种抗菌剂.1 Ml尿样中加入β-葡萄糖醛酸酶-乙酸铵缓冲溶液和混合内标工作液置于37℃水浴酶解过夜(≥16 h).样品采用Oasis HLB固相萃取柱富集净化,在乙腈-水流动相体系下采用Acquity BEH C18色谱柱进行色谱分离,负离子电喷雾(ESI-)多反应监测(MRM)模式检测,稳定同位素内标法定量.实验通过优化仪器质谱参数、流动相和色谱柱等色谱条件以及酶解和固相萃取柱等前处理条件,获得了最佳实验结果.在优化条件下,羟苯甲酯(MeP)和二苯酮-3(BP-3)在4.00～800μg/L,三氯生(TCS)在5.00～200μg/L,其余9种PCPs在1.00～200μg/L范围内线性关系良好,相关系数大于0.999,方法检出限(MDL)为0.06～1.09μg/L,方法定量限(MQL)为0.08～3.63μg/L.12种分析物的加标回收率为89.5％～111.8％,日内精密度为3.7％～8.9％,日间精密度为2.0％～10.6％.采用稳定同位素内标法校正后12种分析物的基质效应为91.9％～110.1％.该方法成功应用于127份实际尿液样品的测定.结果表明,除羟苯苄酯(BzP)和二苯酮-8(BP-8)未检出外,其余10种典型PCPs均可检出,总体检出率为1.7％～99.7％,其中MeP、羟苯乙酯(EtP)和羟苯丙酯(PrP)检出率和浓度水平处于较高水平.该方法简便灵敏,可为人群尿液样本PCPs的生物监测工作提供可靠的技术支持. |
| Author | 张续 张海婧 邱天 林潇 朱英 韩林学 胡小键 |
| AuthorAffiliation | 中国疾病预防控制中心环境与健康相关产品安全所,中国疾病预防控制中心环境与人群健康重点实验室,北京100021 |
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| Author_FL | ZHANG Xu ZHU Ying HU Xiaojian HAN Linxue LIN Xiao QIU Tian ZHANG Haijing |
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| Author_xml | – sequence: 1 fullname: 韩林学 – sequence: 2 fullname: 张续 – sequence: 3 fullname: 胡小键 – sequence: 4 fullname: 张海婧 – sequence: 5 fullname: 邱天 – sequence: 6 fullname: 林潇 – sequence: 7 fullname: 朱英 |
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| Keywords | 超高效液相色谱-串联质谱 尿液 固相萃取 个人护理品 生物监测 |
| Language | Chinese English |
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| Publisher | 中国疾病预防控制中心环境与健康相关产品安全所,中国疾病预防控制中心环境与人群健康重点实验室,北京100021 Editorial board of Chinese Journal of Chromatography |
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| Snippet | O658; 研究建立了一种超高效液相色谱-串联质谱法(UPLC-MS/MS)同时测定人尿中12种典型个人护理品(PCPs)的分析方法,其中包括5种对羟基苯甲酸酯类防腐剂(PBs)、5种二苯酮类紫外吸收剂(BPs)和2种抗菌剂.1... 研究建立了一种超高效液相色谱-串联质谱法(UPLC-MS/MS)同时测定人尿中12种典型个人护理品(PCPs)的分析方法,其中包括5种对羟基苯甲酸酯类防腐剂(PBs)、5种二苯酮类紫外吸收剂(BPs)和2种抗菌剂。1 mL尿样中加入 β -葡萄糖醛酸酶-乙酸铵缓冲溶液和混合内标工作液置于37 ℃水浴酶解过夜(≥16... |
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| Title | 超高效液相色谱-串联质谱法测定人尿中12种典型个人护理品 |
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