酸枣仁种皮和种仁化学成分的定性及半定量分析
O658; 酸枣仁为鼠李科植物酸枣Ziziphus jujuba Mill.var.spinosa(Bunge)Hu ex H.F.Chou的干燥成熟种子,其分为种皮、种仁两个部位,比较研究酸枣仁不同部位化学成分组成及相对含量可为合理开发利用中药酸枣仁资源提供理论依据.基于超高效液相色谱-四极杆飞行时间质谱(UPLC-Q-TOF/MS)技术,从种皮、种仁中共鉴定出57个化学成分.结合主成分分析(PCA)和正交偏最小二乘法判别分析(OPLS-DA)对两者进行差异成分研究,以变量投影重要度(VIP)值＞5为标准,筛选了差异成分17个,其中白桦脂酸、桦木酮酸、麦珠子酸和酸枣仁皂苷Ⅰ主要存在于种皮部位...
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| Published in | Sepu Vol. 42; no. 3; pp. 234 - 244 |
|---|---|
| Main Authors | , , , , , , , , , , |
| Format | Journal Article |
| Language | Chinese English |
| Published |
No. 457, Zhongshan Road, Shahekou District, Dalian City, Liaoning Province
军事科学院军事医学研究院辐射医学研究所,北京 100850%军事科学院军事医学研究院辐射医学研究所,北京 100850%北京中医药大学,北京 100029
08.03.2024
天津中医药大学,天津 301617 Editorial board of Chinese Journal of Chromatography |
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| Online Access | Get full text |
| ISSN | 1000-8713 1872-2059 |
| DOI | 10.3724/SP.J.1123.2023.09015 |
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| Abstract | O658; 酸枣仁为鼠李科植物酸枣Ziziphus jujuba Mill.var.spinosa(Bunge)Hu ex H.F.Chou的干燥成熟种子,其分为种皮、种仁两个部位,比较研究酸枣仁不同部位化学成分组成及相对含量可为合理开发利用中药酸枣仁资源提供理论依据.基于超高效液相色谱-四极杆飞行时间质谱(UPLC-Q-TOF/MS)技术,从种皮、种仁中共鉴定出57个化学成分.结合主成分分析(PCA)和正交偏最小二乘法判别分析(OPLS-DA)对两者进行差异成分研究,以变量投影重要度(VIP)值＞5为标准,筛选了差异成分17个,其中白桦脂酸、桦木酮酸、麦珠子酸和酸枣仁皂苷Ⅰ主要存在于种皮部位,斯皮诺素、酸枣仁皂苷A和6"-阿魏酰斯皮诺素等13个化合物主要存在于种仁部位.通过超高效液相色谱-电雾式检测器(UPLC-CAD)结合反梯度补偿技术,建立半定量液相色谱指纹图谱,考察了6个不同结构类型的代表成分的响应一致性,其不同浓度下平均响应因子间的RSD值为7.04％,各化合物响应一致性良好,可用于酸枣仁的半定量表征分析;结果表明:种皮部位主要成分为白桦脂酸和油酸,其中白桦脂酸的含量约是种仁的7倍;种仁部位主要成分为斯皮诺素、酸枣仁皂苷A、亚油酸、白桦脂酸和油酸,其中斯皮诺素、酸枣仁皂苷A的含量分别是种皮的18倍和24倍.综上,本研究阐明了酸枣仁种皮、种仁的化学成分差异,明确了酸枣仁两个部位中各自的主要成分及其相对含量,为酸枣仁不同部位合理开发和利用奠定了基础. |
|---|---|
| AbstractList | 酸枣仁为鼠李科植物酸枣
Ziziphus jujuba
Mill. var.
spinosa
(Bunge) Hu ex H. F. Chou的干燥成熟种子,其分为种皮、种仁两个部位,比较研究酸枣仁不同部位化学成分组成及相对含量可为合理开发利用中药酸枣仁资源提供理论依据。基于超高效液相色谱-四极杆飞行时间质谱(UPLC-Q-TOF/MS)技术,从种皮、种仁中共鉴定出57个化学成分。结合主成分分析(PCA)和正交偏最小二乘法判别分析(OPLS-DA)对两者进行差异成分研究,以变量投影重要度(VIP)值> 5为标准,筛选了差异成分17个,其中白桦脂酸、桦木酮酸、麦珠子酸和酸枣仁皂苷Ⅰ主要存在于种皮部位,斯皮诺素、酸枣仁皂苷A和6‴-阿魏酰斯皮诺素等13个化合物主要存在于种仁部位。通过超高效液相色谱-电雾式检测器(UPLC-CAD)结合反梯度补偿技术,建立半定量液相色谱指纹图谱,考察了6个不同结构类型的代表成分的响应一致性,其不同浓度下平均响应因子间的RSD值为7.04%,各化合物响应一致性良好,可用于酸枣仁的半定量表征分析;结果表明:种皮部位主要成分为白桦脂酸和油酸,其中白桦脂酸的含量约是种仁的7倍;种仁部位主要成分为斯皮诺素、酸枣仁皂苷A、亚油酸、白桦脂酸和油酸,其中斯皮诺素、酸枣仁皂苷A的含量分别是种皮的18倍和24倍。综上,本研究阐明了酸枣仁种皮、种仁的化学成分差异,明确了酸枣仁两个部位中各自的主要成分及其相对含量,为酸枣仁不同部位合理开发和利用奠定了基础。 O658; 酸枣仁为鼠李科植物酸枣Ziziphus jujuba Mill.var.spinosa(Bunge)Hu ex H.F.Chou的干燥成熟种子,其分为种皮、种仁两个部位,比较研究酸枣仁不同部位化学成分组成及相对含量可为合理开发利用中药酸枣仁资源提供理论依据.基于超高效液相色谱-四极杆飞行时间质谱(UPLC-Q-TOF/MS)技术,从种皮、种仁中共鉴定出57个化学成分.结合主成分分析(PCA)和正交偏最小二乘法判别分析(OPLS-DA)对两者进行差异成分研究,以变量投影重要度(VIP)值＞5为标准,筛选了差异成分17个,其中白桦脂酸、桦木酮酸、麦珠子酸和酸枣仁皂苷Ⅰ主要存在于种皮部位,斯皮诺素、酸枣仁皂苷A和6"-阿魏酰斯皮诺素等13个化合物主要存在于种仁部位.通过超高效液相色谱-电雾式检测器(UPLC-CAD)结合反梯度补偿技术,建立半定量液相色谱指纹图谱,考察了6个不同结构类型的代表成分的响应一致性,其不同浓度下平均响应因子间的RSD值为7.04％,各化合物响应一致性良好,可用于酸枣仁的半定量表征分析;结果表明:种皮部位主要成分为白桦脂酸和油酸,其中白桦脂酸的含量约是种仁的7倍;种仁部位主要成分为斯皮诺素、酸枣仁皂苷A、亚油酸、白桦脂酸和油酸,其中斯皮诺素、酸枣仁皂苷A的含量分别是种皮的18倍和24倍.综上,本研究阐明了酸枣仁种皮、种仁的化学成分差异,明确了酸枣仁两个部位中各自的主要成分及其相对含量,为酸枣仁不同部位合理开发和利用奠定了基础. |
| Abstract_FL | Ziziphi Spinosae Semen refers to the dried seed of Ziziphus jujuba Mill.var.spinosa(Bunge)Hu ex H.F.Chou.The seed is composed of a reddish brown coat and a yellow kernel.A comparative study was conducted to investigate differences in the chemical composition and their relative contents between the seed coat and kernel of Ziziphi Spinosae Semen.First,the chemical compounds found in the seed coat and kernel were characterized and identified using ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF/MS).The analytical results tentatively identified 57 chemical compounds based on ref-erence-compound comparison,literature retrieval,and chemical-database(e.g.,MassBank)searches;these compounds included 14 triterpenes,23 flavonoids,7 alkaloids,6 carboxylic acids,and 7 other types of compounds.The mass error of the identified compounds was within the mass deviation range of 5x10-6(5 ppm).Next,two methods of multivariate statistical anal-ysis,namely,principal component analysis(PCA)and orthogonal partial least squares-discrim-inant analysis(OPLS-DA),were used to compare the differential compounds between the two seed parts.A total of 17 differential compounds were screened out via OPLS-DA based on a var-iable importance in projection(VIP)value of＞5.The results revealed that betulinic acid,betu-lonic acid,alphitolic acid,and jujuboside Ⅰ mainly existed in the seed coat whereas the 13 other compounds,such as spinosin,jujuboside A,and 6"'-feruloylspinosin,mainly existed in the seed kernel.Therefore,these 17 differential compounds can be used to distinguish between the two seed parts.Finally,a semiquantitative method was established using UPLC and a charged aerosol detector(CAD)with inverse gradient compensation in the mobile phase.Six representative compounds with different types were selected to examine the CAD response con-sistency:magnoflorine(alkaloid),spinosin(flavone),6"'-feruloylspinosin(flavone),jujubo-side A(triterpenoid saponin),jujuboside B(triterpenoid saponin),and betulinic acid(triter-penoid acid).The results showed that the relative standard deviation(RSD)of the average re-sponse factors at different levels of these six compounds was 7.04％and that their response in-tensities were similar.Moreover,each compound in the fingerprint demonstrated good response consistency,and the peak areas obtained directly reflected the contents of each compound.Based on the semiquantitative fingerprints obtained,betulinic acid and oleic acid were consid-ered the main components of the seed coat.The betulinic acid content in the seed coat was ap-proximately 7 times higher than that in the seed kernel.Spinosin,jujuboside A,linoleic acid,betulinic acid,and oleic acid were the main components of the seed kernel.The spinosin con-tent in the seed kernel was 18 times higher than that in the seed coat.In addition,the jujubo-side A content in the seed kernel was 24 times higher than that in the seed coat.The proposed method can accurately determine the main components and compare the relative contents of these components in different seed parts.In summary,this study identified the differences in chemical components between the seed coat and kernel of Ziziphi Spinosae Semen and clarified the main components and their relative contents in these parts.The findings can not only pro-vide a basis for the identification of chemical compounds and quality research on different parts of Ziziphi Spinosae Semen but also promote the development and utilization of this traditional Chinese medicine. |
| Author | 梁海珍 马百平 陈晓娟 贾德贤 王谦 南易 石宇浩 张洁 姚兰 郑伟 宋娟 |
| AuthorAffiliation | 天津中医药大学,天津 301617;军事科学院军事医学研究院辐射医学研究所,北京 100850%军事科学院军事医学研究院辐射医学研究所,北京 100850%北京中医药大学,北京 100029 |
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| Author_FL | SONG Juan LIANG Haizhen NAN Yi CHEN Xiaojuan WANG Qian ZHANG Jie MA Baiping SHI Yuhao YAO Lan JIA Dexian ZHENG Wei |
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| DocumentTitle_FL | Qualitative and semiquantitative analyses of the chemical components of the seed coat and kernel of Ziziphi Spinosae Semen |
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| Keywords | seed kernel charged aerosol detector(CAD) mass spectrometry(MS) 电雾式检测器 差异成分 半定量分析 种皮 seed coat ultra performance liquid chromatography(UPLC) difference markers 超高效液相色谱 Ziziphi Spinosae Semen semiquantitative analysis 质谱 酸枣仁 种仁 |
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| Title | 酸枣仁种皮和种仁化学成分的定性及半定量分析 |
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