Histone H2B Deubiquitination Is Required for Transcriptional Activation of FLOWERING LOCUS C and for Proper Control of Flowering in Arabidopsis1[C][W][OA]
The spectrum of histone modifications at a given locus is a critical determinant for the correct output of gene expression. In Arabidopsis (Arabidopsis thaliana), many studies have examined the relationship between histone methylation and gene expression, but few studies exist on the relationship be...
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Published in | Plant physiology (Bethesda) Vol. 149; no. 2; pp. 1196 - 1204 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Rockville
American Society of Plant Biologists
01.02.2009
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Abstract | The spectrum of histone modifications at a given locus is a critical determinant for the correct output of gene expression. In Arabidopsis (Arabidopsis thaliana), many studies have examined the relationship between histone methylation and gene expression, but few studies exist on the relationship between other covalent histone modifications and gene expression. In this work, we describe the role of histone H2B deubiquitination in the activation of gene expression and the consequence of a perturbation of histone H2B deubiquitination in the timing of the floral transition in Arabidopsis. A mutation in a H2B deubiquitinase, UBIQUITIN-SPECIFIC PROTEASE26 (UBP26), results in an early-flowering phenotype. In the ubp26 mutant, mRNA levels of the floral repressor FLOWERING LOCUS C (FLC) and other related family members is decreased. Furthermore, this mutant accumulates H2B monoubiquitination, and has decreased levels of H3K36 trimethylation and increased levels of H3K27 trimethylation at the FLC locus. Thus, UBP26 is required for transcriptional activation of FLC through H2B deubiquitination and is consistent with a model in which deubiquitination is necessary for the accumulation of H3K36 trimethylation and the proper level of transcriptional activation. |
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AbstractList | The spectrum of histone modifications at a given locus is a critical determinant for the correct output of gene expression. In Arabidopsis (Arabidopsis thaliana), many studies have examined the relationship between histone methylation and gene expression, but few studies exist on the relationship between other covalent histone modifications and gene expression. In this work, we describe the role of histone H2B deubiquitination in the activation of gene expression and the consequence of a perturbation of histone H2B deubiquitination in the timing of the floral transition in Arabidopsis. A mutation in a H2B deubiquitinase, UBIQUITIN-SPECIFIC PROTEASE26 (UBP26), results in an early-flowering phenotype. In the ubp26 mutant, mRNA levels of the floral repressor FLOWERING LOCUS C (FLC) and other related family members is decreased. Furthermore, this mutant accumulates H2B monoubiquitination, and has decreased levels of H3K36 trimethylation and increased levels of H3K27 trimethylation at the FLC locus. Thus, UBP26 is required for transcriptional activation of FLC through H2B deubiquitination and is consistent with a model in which deubiquitination is necessary for the accumulation of H3K36 trimethylation and the proper level of transcriptional activation. The spectrum of histone modifications at a given locus is a critical determinant for the correct output of gene expression. In Arabidopsis ( Arabidopsis thaliana ), many studies have examined the relationship between histone methylation and gene expression, but few studies exist on the relationship between other covalent histone modifications and gene expression. In this work, we describe the role of histone H2B deubiquitination in the activation of gene expression and the consequence of a perturbation of histone H2B deubiquitination in the timing of the floral transition in Arabidopsis. A mutation in a H2B deubiquitinase, UBIQUITIN - SPECIFIC PROTEASE26 ( UBP26 ), results in an early-flowering phenotype. In the ubp26 mutant, mRNA levels of the floral repressor FLOWERING LOCUS C ( FLC ) and other related family members is decreased. Furthermore, this mutant accumulates H2B monoubiquitination, and has decreased levels of H3K36 trimethylation and increased levels of H3K27 trimethylation at the FLC locus. Thus, UBP26 is required for transcriptional activation of FLC through H2B deubiquitination and is consistent with a model in which deubiquitination is necessary for the accumulation of H3K36 trimethylation and the proper level of transcriptional activation. |
Author | Amasino, Richard Mark Doyle, Mark Robert Schmitz, Robert Jeffrey Tamada, Yosuke Zhang, Xiaoyu |
AuthorAffiliation | Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706 (R.J.S., Y.T., M.R.D., R.M.A.); and Department of Plant Biology, University of Georgia, Athens, Georgia 30602 (X.Z.) |
AuthorAffiliation_xml | – name: Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706 (R.J.S., Y.T., M.R.D., R.M.A.); and Department of Plant Biology, University of Georgia, Athens, Georgia 30602 (X.Z.) |
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Copyright | Copyright American Society of Plant Biologists Feb 2009 Copyright © 2009, American Society of Plant Biologists |
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DOI | 10.1104/pp.108.131508 |
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Notes | Some figures in this article are displayed in color online but in black and white in the print edition. This work was supported by the College of Agricultural and Life Sciences and the Graduate School of the University of Wisconsin (National Institutes of Health grant no. 1R01GM079525 and National Science Foundation grant no. 0209786 [to R.M.A.]). Corresponding author; e-mail amasino@biochem.wisc.edu. These authors contributed equally to the article. The author responsible for the distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Richard Mark Amasino (amasino@biochem.wisc.edu). Open access articles can be viewed online without a subscription. Present address: Plant Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037. The online version of this article contains Web-only data. www.plantphysiol.org/cgi/doi/10.1104/pp.108.131508 |
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Title | Histone H2B Deubiquitination Is Required for Transcriptional Activation of FLOWERING LOCUS C and for Proper Control of Flowering in Arabidopsis1[C][W][OA] |
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