Localising elements in single-particle reconstructions by REEL-EM: Reconstructed Electron Energy-Loss - Elemental Mapping

• Published in: bioRxiv
• Main Authors: Pfeil-Gardiner, Olivia, Higor Vinicius Dias Rosa, Riedel, Dietmar, Chen, Yu Seby, Lorks, Dominique, Kukelhan, Pirmin, Linck, Martin, Muller, Heiko, Filip Van Petegem, Murphy, Bonnie J
• Format: Paper
• Language: English
• Published: Cold Spring Harbor Cold Spring Harbor Laboratory Press 18.01.2024
• Subjects: Image processing; Lipids; Macromolecules; Mapping; Metals; Spectroscopy

For structures determined by single particle cryo-EM, no technique currently exists for mapping elements to defined locations, leading to errors in the assignment of metals and other ions, cofactors, substrates, inhibitors, and lipids that play essential roles in activity and regulation. Elemental m...