The highly repetitive genome of Myxobolus sp., a myxozoan parasite of fathead minnows

• Published in: bioRxiv
• Main Authors: Muthye, Viraj R, Aralia Leon Coria, Liu, Hongrui, Finney, Constance A M, Goater, Cameron P, Wasmuth, James D
• Format: Paper
• Language: English
• Published: Cold Spring Harbor Cold Spring Harbor Laboratory Press 17.02.2024
• Subjects: Economic importance; Gene families; Genes; Genomes; Host-parasite interactions; Juveniles; Mitochondria; Myxobolus; Parasites; Serine proteinase; Whirling disease
• DOI: 10.1101/2024.02.09.579586

Background: The Myxozoa is a group of at least 2,400 endoparasites within the phylum Cnidaria. All myxozoans have greatly reduced in size and morphology compared to free-living members of the phylum. They are best known for causing disease in economically important fish across the world; for example, Myxobolus cerebralis causes Whirling Disease, which can kill 90% of infected juvenile salmonid fish. In 2017, a potentially new myxozoan species was identified in Alberta. Myxobolus sp. causes distinct lesions in fathead minnows, which are ultimately fatal. Here, we sequenced, assembled and analyzed the genome of Myxobolus sp. to understand how the parasite interacts with its fish host and identify potential strategies to counter this emerging threat. Results: At 185 Mb, the Myxobolus sp. genome is the largest myxozoan genome sequenced so far. This large genome size is, in part, due to the high repetitive content; 68% of the genome was interspersed repeats, with the MULE-MuDR transposon covering 18% of the Myxobolus sp. genome. Similar to myxozoan genomes, the Myxobolus sp. genome has lost many genes well conserved in other eukaryotes. However, we also identified multiple expansions in gene families (serine proteases, hexokinases, and FLYWCH-domain containing proteins) which suggests their functional importance in the parasite. The mitochondrial genome of Myxobolus sp. encodes only five of the thirteen protein-coding genes typically found in animals. We found that the mitochondrial gene atp6 was transferred to the nucleus and acquired a mitochondria-targeting signal in Myxobolus sp. Conclusions: Our study provides valuable insights into myxozoan biology and identify promising avenues for future research. We also propose that M. rasmusseni is promising myxozoan model to explore host-parasite interactions in these parasites.Competing Interest StatementThe authors have declared no competing interest.Footnotes* In a previous version of this preprint, we used a species epithet that is under peer-review (February 2024). Our inclusion of it in the preprint was not intended for the purposes of zoological nomenclature. We have removed it in this current version. We made the necessary changes and added an explanatory sentence in the new version.