Quantitative data independent acquisition glycoproteomics of sparkling wine

Sparkling wine is an alcoholic beverage enjoyed around the world. The sensory properties of sparkling wine depend on a complex interplay between the chemical and biochemical components in the final product. Glycoproteins have been linked to positive and negative qualities in sparkling wine, but the...

Full description

Saved in:
Bibliographic Details
Published inbioRxiv
Main Authors Pegg, Cassandra L, Phung, Toan K, Caboche, Christopher H, Niamsuphap, Suchada, Marshall, Bern, Howell, Kate, Schulz, Benjamin L
Format Paper
LanguageEnglish
Published Cold Spring Harbor Cold Spring Harbor Laboratory Press 26.08.2020
Subjects
Online AccessGet full text

Cover

Loading…
Abstract Sparkling wine is an alcoholic beverage enjoyed around the world. The sensory properties of sparkling wine depend on a complex interplay between the chemical and biochemical components in the final product. Glycoproteins have been linked to positive and negative qualities in sparkling wine, but the glycosylation profiles of sparkling wine have not been previously investigated in detail. We analysed the glyco/proteome of sparkling wines using protein- and glycopeptide-centric approaches. We developed an automated workflow that created ion libraries to analyse Sequential Window Acquisition of all THeoretical mass spectra (SWATH) Data Independent Acquisition (DIA) mass spectrometry data based on glycopeptides identified by Byonic. We applied our workflow to three pairs of experimental sparkling wines to assess the effects of aging on lees and of different yeast strains used in the Liqueur de Tirage for secondary fermentation. We found that aging a cuvee on lees for 24 months compared to 8 months led to a dramatic decrease in overall protein abundance and an enrichment in large glycans at specific sites in some proteins. Secondary fermentation of a Riesling wine with Saccharomyces cerevisiae yeast strain Siha4 produced more yeast proteins and glycoproteins than with S. cerevisiae yeast strain DV10. The abundance and glycosylation profiles of grape glycoproteins were also different between grape varieties. This work represents the first in-depth study into protein- and peptide-specific glycosylation in sparkling wines and describes a quantitative glycoproteomic SWATH/DIA workflow that is broadly applicable to other sample types. Competing Interest Statement The authors have declared no competing interest.
AbstractList Sparkling wine is an alcoholic beverage enjoyed around the world. The sensory properties of sparkling wine depend on a complex interplay between the chemical and biochemical components in the final product. Glycoproteins have been linked to positive and negative qualities in sparkling wine, but the glycosylation profiles of sparkling wine have not been previously investigated in detail. We analysed the glyco/proteome of sparkling wines using protein- and glycopeptide-centric approaches. We developed an automated workflow that created ion libraries to analyse Sequential Window Acquisition of all THeoretical mass spectra (SWATH) Data Independent Acquisition (DIA) mass spectrometry data based on glycopeptides identified by Byonic. We applied our workflow to three pairs of experimental sparkling wines to assess the effects of aging on lees and of different yeast strains used in the Liqueur de Tirage for secondary fermentation. We found that aging a cuvee on lees for 24 months compared to 8 months led to a dramatic decrease in overall protein abundance and an enrichment in large glycans at specific sites in some proteins. Secondary fermentation of a Riesling wine with Saccharomyces cerevisiae yeast strain Siha4 produced more yeast proteins and glycoproteins than with S. cerevisiae yeast strain DV10. The abundance and glycosylation profiles of grape glycoproteins were also different between grape varieties. This work represents the first in-depth study into protein- and peptide-specific glycosylation in sparkling wines and describes a quantitative glycoproteomic SWATH/DIA workflow that is broadly applicable to other sample types. Competing Interest Statement The authors have declared no competing interest.
Author Pegg, Cassandra L
Caboche, Christopher H
Niamsuphap, Suchada
Phung, Toan K
Marshall, Bern
Howell, Kate
Schulz, Benjamin L
Author_xml – sequence: 1
  givenname: Cassandra
  surname: Pegg
  middlename: L
  fullname: Pegg, Cassandra L
– sequence: 2
  givenname: Toan
  surname: Phung
  middlename: K
  fullname: Phung, Toan K
– sequence: 3
  givenname: Christopher
  surname: Caboche
  middlename: H
  fullname: Caboche, Christopher H
– sequence: 4
  givenname: Suchada
  surname: Niamsuphap
  fullname: Niamsuphap, Suchada
– sequence: 5
  givenname: Bern
  surname: Marshall
  fullname: Marshall, Bern
– sequence: 6
  givenname: Kate
  surname: Howell
  fullname: Howell, Kate
– sequence: 7
  givenname: Benjamin
  surname: Schulz
  middlename: L
  fullname: Schulz, Benjamin L
BookMark eNotjr1OwzAURj3AAIUHYLPEnHCvndhkRBV_ohJC6l5d7JvKEOw0dkC8PZHo8p3tnO9cnMQUWYgrhBoR8EaBghpMDV2NDSplzsTL20yxhEIlfLP0VEiG6HnkZWKR5A5zyKGEFOV--HVpnFLh9BVclqmXeaTpcwhxL39C5Atx2tOQ-fLIldg-3G_XT9Xm9fF5fbepRoum6gC8AWfYsybWhsCQY904r1jpjluwXnnX9uQa37DB3mlHrbHKUMv4rlfi-l-7fDnMnMvuI81TXIo71SDq1lp1q_8AqMZMYA
ContentType Paper
Copyright 2020. This article is published under http://creativecommons.org/licenses/by/4.0/ (“the License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
Copyright_xml – notice: 2020. This article is published under http://creativecommons.org/licenses/by/4.0/ (“the License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
DBID 8FE
8FH
AAFGM
AAMXL
ABOIG
ABUWG
ADZZV
AFKRA
AFLLJ
AFOLM
AGAJT
AQTIP
AZQEC
BBNVY
BENPR
BHPHI
CCPQU
DWQXO
GNUQQ
HCIFZ
LK8
M7P
PIMPY
PQCXX
PQEST
PQQKQ
PQUKI
PRINS
DOI 10.1101/2020.06.09.141226
DatabaseName ProQuest SciTech Collection
ProQuest Natural Science Collection
ProQuest Central Korea - hybrid linking
Natural Science Collection - hybrid linking
Biological Science Collection - hybrid linking
ProQuest Central (Alumni)
ProQuest Central (Alumni) - hybrid linking
ProQuest Central
SciTech Premium Collection - hybrid linking
ProQuest Central Student - hybrid linking
ProQuest Central Essentials - hybrid linking
ProQuest Women's & Gender Studies - hybrid linking
ProQuest Central Essentials
Biological Science Collection
ProQuest Central
Natural Science Collection
ProQuest One Community College
ProQuest Central Korea
ProQuest Central Student
SciTech Premium Collection
Biological Sciences
Biological Science Database
Publicly Available Content Database
ProQuest Central - hybrid linking
ProQuest One Academic Eastern Edition (DO NOT USE)
ProQuest One Academic
ProQuest One Academic UKI Edition
ProQuest Central China
DatabaseTitle Publicly Available Content Database
ProQuest Central Student
ProQuest Biological Science Collection
ProQuest Central Essentials
ProQuest One Academic Eastern Edition
ProQuest Central (Alumni Edition)
SciTech Premium Collection
ProQuest One Community College
ProQuest Natural Science Collection
Biological Science Database
ProQuest SciTech Collection
ProQuest Central China
ProQuest Central
ProQuest One Academic UKI Edition
Natural Science Collection
ProQuest Central Korea
Biological Science Collection
ProQuest One Academic
DatabaseTitleList Publicly Available Content Database
Database_xml – sequence: 1
  dbid: BENPR
  name: ProQuest Central
  url: https://www.proquest.com/central
  sourceTypes: Aggregation Database
DeliveryMethod fulltext_linktorsrc
Genre Working Paper/Pre-Print
GroupedDBID 8FE
8FH
ABUWG
AFKRA
AZQEC
BBNVY
BENPR
BHPHI
CCPQU
DWQXO
GNUQQ
HCIFZ
LK8
M7P
PIMPY
PQEST
PQQKQ
PQUKI
PRINS
ID FETCH-LOGICAL-p716-900d60c6ede3ae36a06ace34cd2e239e507d2dc5fac4d4e61fc3ca56726a5e1b3
IEDL.DBID BENPR
IngestDate Thu Oct 10 20:16:38 EDT 2024
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed false
IsScholarly false
Language English
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-p716-900d60c6ede3ae36a06ace34cd2e239e507d2dc5fac4d4e61fc3ca56726a5e1b3
OpenAccessLink https://www.proquest.com/docview/2411357728?pq-origsite=%requestingapplication%
PQID 2411357728
PQPubID 2050091
ParticipantIDs proquest_journals_2411357728
PublicationCentury 2000
PublicationDate 20200826
PublicationDateYYYYMMDD 2020-08-26
PublicationDate_xml – month: 08
  year: 2020
  text: 20200826
  day: 26
PublicationDecade 2020
PublicationPlace Cold Spring Harbor
PublicationPlace_xml – name: Cold Spring Harbor
PublicationTitle bioRxiv
PublicationYear 2020
Publisher Cold Spring Harbor Laboratory Press
Publisher_xml – name: Cold Spring Harbor Laboratory Press
Score 1.6425258
Snippet Sparkling wine is an alcoholic beverage enjoyed around the world. The sensory properties of sparkling wine depend on a complex interplay between the chemical...
SourceID proquest
SourceType Aggregation Database
SubjectTerms Aging
Alcoholic beverages
Fermentation
Glycopeptides
Glycoproteins
Glycosylation
Mass spectroscopy
Polysaccharides
Proteins
Proteomes
Saccharomyces cerevisiae
Sensory properties
Sparkling wine
Wine
Yeast
Title Quantitative data independent acquisition glycoproteomics of sparkling wine
URI https://www.proquest.com/docview/2411357728
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1LSwMxEA7aXrwpKj6q5OA1mM2ruydBaSkKpUqF3ko2mS0F2d1uW6T_3sma6kEQcgsEMgzfvOcj5I6bHI9TTAjumRJ9YBm6zSyXmQIwhU2LtttibEbv6nmmZzHhto5tlXtMbIHaVy7kyO_R0iRSoy-YPtQrFlijQnU1Umgckq5IZJp2SPdxMJ68xfIlqlsI7nm7oTNDUEiEMH9At7Ukw2PSndgamhNyAOUpeXnd2rId80LQoaFbky5_eGk31LrVdvndVEUXHztXtVsVwhzxmlYFRTBoAuH6gn6ir3hGpsPB9GnEIr0BqzFIYRnn3nBnwIO0II3lxjqQynkBQmaAjpoX3unCOuUVmKRw0llt-sJYDUkuz0mnrEq4IFR4CRag4FJ6JTGESvEdjYGMBuUzri5Jb__leVTR9fxXoFf_X1-ToyDEkEgVpkc6m2YLN2iJN_ltFPcX3b-Mtw
link.rule.ids 780,784,21388,27925,33744,43805
linkProvider ProQuest
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1LSwMxEA7aHvSmqPiomoPXYDavdk-C0lJtLVUq9FayyWwpyG6fiP_eyZrqQRByCwQyDN-85yPkhpsMj1NMCO6ZEk1gKbrNLJOpAjC5beVVt8XAdN_U01iPY8JtFdsqt5hYAbUvXciR36KlSaRGX7B1N1-wwBoVqquRQmOX1MPmdF0j9fv2YPgay5eobiG459WGzhRBIRHC_AHdypJ0Dkh9aOewPCQ7UByR3svGFtWYF4IODd2adPbDS7um1i02s--mKjp9_3RltVUhzBGvaJlTBINlIFyf0g_0FY_JqNMePXRZpDdgcwxSWMq5N9wZ8CAtSGO5sQ6kcl6AkCmgo-aFdzq3TnkFJsmddFabpjBWQ5LJE1IrygJOCRVeggXIuZReSQyhWviOxkBGg_IpV2eksf3yJKroavIr0PP_r6_JXnf03J_0Hwe9C7IfBBqSqsI0SG293MAlWuV1dhVF_wWS3o-f
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Quantitative+data+independent+acquisition+glycoproteomics+of+sparkling+wine&rft.jtitle=bioRxiv&rft.au=Pegg%2C+Cassandra+L&rft.au=Phung%2C+Toan+K&rft.au=Caboche%2C+Christopher+H&rft.au=Niamsuphap%2C+Suchada&rft.date=2020-08-26&rft.pub=Cold+Spring+Harbor+Laboratory+Press&rft_id=info:doi/10.1101%2F2020.06.09.141226