Cryogenic X-ray crystallographic studies of biomacromolecules at Turkish Light Source: Turkish DeLight

• Published in: bioRxiv
• Main Authors: Atalay, Necati, Enver Kamil Akcan, Gul, Mehmet, Ayan, Esra, Destan, Ebru, Ertem, Fatma Betul, Tokay, Nurettin, Cakilkaya, Baris, Zelis Nergiz, Karakadioglu, Gozde, Kepceoglu, Abdullah, Yapici, Ilkin, Tosun, Bilge, Baldir, Nilufer, Yildirim, Gunseli, J Austin Johnson, Guven, Omur, Shafiei, Alaleh, Arslan, Nazli Eylul, Yilmaz, Merve, Kulakman, Cahine, Seyide Seda Paydos, Zeynep Sena Cinal, Sabanoglu, Kardelen, Pazarceviren, Aysegul, Yilmaz, Aysenur, Canbay, Basak, Asci, Bengisu, Kartal, Esra, Serra Tavli, Caliseki, Mehmet, Goc, Gunce, Mermer, Arif, Yesilay, Gamze, Altuntas, Sevde, Tateishi, Hiroshi, Otsuka, Masami, Fujita, Mikako, Saban Tekin, Ciftci, Halilibrahim, Durdagi, Serdar, Doganay, Gizem Dinler, Karaca, Ezgi, Burcu Kaplan Turkoz, Burak Veli Kabasakal, Kati, Ahmet, Demirci, Hasan
• Format: Paper
• Language: English
• Published: Cold Spring Harbor Cold Spring Harbor Laboratory Press 05.09.2022
• Subjects: Crystallography; Crystals; Lysozyme; Macromolecules; Proteins; X-ray crystallography; X-ray diffraction; Turkey
• DOI: 10.1101/2022.09.03.506456

X-ray crystallography is a robust and powerful structural biology technique that provides high-resolution atomic structures of biomacromolecules. Scientists use this technique to unravel mechanistic and structural details of biological macromolecules (e.g. proteins, nucleic acids, protein complexes, protein-nucleic acid complexes, or large biological compartments). Since its inception, single-crystal cryo-crystallography has never been performed in Turkiye due to the lack of a single-crystal X-ray diffractometer. The X-ray diffraction facility recently established at the University of Health Sciences, Istanbul, Turkiye will enable Turkish and international researchers to easily perform high-resolution structural analysis of biomacromolecules from single crystals. Here, we describe the technical and practical outlook of a state-of-the-art home-source X-ray, using lysozyme as a model protein. The methods and practice described in this article can be applied to any biological sample for structural studies. Therefore, this article will be a valuable practical guide from sample preparation to data analysis. Competing Interest Statement The authors have declared no competing interest. Footnotes * https://www.rcsb.org/structure/unreleased/7Y6A