Electromembrane extraction and mass spectrometry for liver organoid drug metabolism studies

• Published in: bioRxiv
• Main Authors: Frøydis Sved Skottvoll, Hansen, Frederik, Harrison, Sean, Boger, Ida Sneis, Ago Mrsa, Magnus Saed Restan, Stein, Matthias, Lundanes, Elsa, Pedersen-Bjergaard, Stig, Aizenshtadt, Aleksandra, Krauss, Stefan, Sullivan, Gareth, Bogen, Inger Lise, Wilson, Steven Ray
• Format: Paper
• Language: English
• Published: Cold Spring Harbor Cold Spring Harbor Laboratory Press 04.12.2020
• Subjects: Biomaterials; Capillary electrophoresis; Chromatography; Drug abuse; Drug development; Drug metabolism; Heroin; Liquid chromatography; Liver; Mass spectrometry; Mass spectroscopy; Metabolism; Metabolites; Organoids; Scientific imaging; Stem cells; Toxicity

Abstract Liver organoids are emerging tools for precision drug development and toxicity screening. We demonstrate that electromembrane extraction (EME) based on electrophoresis across an oil membrane is suited for segregating selected organoid-derived drug metabolites prior to mass spectrometry (MS)...