Nrl is dispensable for specification of rod photoreceptors in adult zebrafish contrasting a deeply conserved requirement earlier in ontogeny

• Published in: bioRxiv
• Main Authors: Oel, A Phillip, Neil, Gavin J, Dong, Emily M, Balay, Spencer D, Collett, Keon, Allison, W Ted
• Format: Paper
• Language: English
• Published: Cold Spring Harbor Cold Spring Harbor Laboratory Press 27.06.2020
• Subjects: Cell fate; Cones; Danio rerio; Leucine zipper proteins; Mutants; NRL protein; Ontogeny; Photoreceptors; Retina; Rods; Zebrafish
• DOI: 10.1101/2020.06.26.173930

The transcription factor NRL (Neural Retinal Leucine-zipper) has been canonized, appropriately enough, as the master regulator of photoreceptor cell fate in the retina. NRL is necessary and sufficient to specify rod cell fate and to preclude cone cell fate in mice. By engineering zebrafish we tested if NRL function has conserved roles beyond mammals or beyond nocturnal species, i.e. in a vertebrate possessing a greater and more typical diversity of cone sub-types. Here, transgenic expression of a Nrl homolog from zebrafish or mouse was sufficient to convert developing zebrafish cones into rod photoreceptors. Zebrafish nrl-/- mutants lacked rods (and had excess UV-sensitive cones) as young larvae, thus the conservation of Nrl function between mice and zebrafish appears sound. These data inform hypotheses of photoreceptor evolution through the Nocturnal Bottleneck, suggesting that a capacity to favor nocturnal vision is a property of NRL that predates the emergence of early mammals. Strikingly, however, rods were abundant in adult nrl-/- null mutant zebrafish. Rods developed in adults despite Nrl protein being undetectable. Therefore a yet-to-be-revealed non-canonical pathway independent of nrl is able to specify the fate of some rod photoreceptors. Competing Interest Statement The authors have declared no competing interest.