Pseudolaric acid B extracted from the Chinese medicinal herb Cortex Pseudolaricis ameliorates DNFB-induced atopic dermatitis-like skin lesions in BALB/c mice

• Published in: Traditional medicine research Vol. 2; no. 4; pp. 169 - 175
• Main Authors: Yi-Teng, Wang, Zhang, Min, Liu, Mei-Lun, Li-Ming, Zhang, Chen, Hong
• Format: Journal Article
• Language: English
• Published: Auckland TMR Publishing Group 01.01.2017; Department of Pharmacognosy and Pharmaceutics, Logistics University of Chinese People's Armed Police Force, Tianjin, China%Department of Science Research, Logistics University of Chinese People's Armed Police Force, Tianjin, China%Department of Pathogen Biology and Immunology, Logistics University of Chinese People's Armed Police Force, Tianjin, China
• Subjects: Acids; Chinese medicine; Dermatitis; Eczema; Immunology; Inflammation; Laboratory animals; Medical research; Pathogens; Pharmaceutical sciences; Skin; NLRP3炎症小体; Atopic dermatitis; Pseudolaric acid B; 特应性皮炎; Inflammation; NLRP3 inflammasome; 土槿乙酸; 炎症反应
• DOI: 10.12032/TMR201708056
• ISSN: 2413-3973

Objective: Pseudolaric acid B (PB) is a newly identified diterpenoid isolated from Tujinpi (Cortex Pseudolaricis). In the present study, we aimed to explore the anti-inflammatory effects of PB on atopic dermatitis (AD), as well as the molecular mechanisms underlying its effects. Methods: BALB/c mice treated with 2,4-dinitrofluorobenzene were orally administered with PB (10 mg×kg-1×d-1). After evaluating the AD score, serum levels of IgE and the mRNA expression of NLRP3 inflammasome and IL-1β were measured by ELISA and qRT-PCR respectively. Results: The results showed that PB treatment significantly ameliorated the development of AD-like clinical symptoms and effectively suppressed the infiltration of inflammatory cells. Furthermore, PB inhibited the expression of NLRP3 inflammasome and IL-1β in skin lesions, and downregulated serum IgE levels. Conclusion: The anti-inflammatory properties of PB were demonstrated using the 2,4-dinitrofluorobenzene-induced mouse model of AD-like skin lesions. Our study highlighted the potential use of PB as a novel therapeutic agent for the treatment of inflammation-associated skin diseases.