Oocyte activation with phospholipase Cζ mRNA induces repetitive intracellular Ca 2+ rises and improves the quality of pig embryos after intracytoplasmic sperm injection

• Published in: The Journal of reproduction and development Vol. 70; no. 4; p. 229
• Main Authors: Nakai, Michiko, Suzuki, Shun-Ichi, Fuchimoto, Dai-Ichiro, Sembon, Shoichiro, Kikuchi, Kazuhiro
• Format: Journal Article
• Language: English
• Published: Japan 07.08.2024
• Subjects: Animals; Blastocyst - metabolism; Calcium - metabolism; Calcium Signaling; Embryonic Development; Female; Fertilization; Fertilization in Vitro - veterinary; Male; Oocytes - metabolism; Phosphoinositide Phospholipase C - genetics; Phosphoinositide Phospholipase C - metabolism; RNA, Messenger - metabolism; Sperm Injections, Intracytoplasmic; Spermatozoa - metabolism; Swine; Oocyte activation; Phospholipase Cζ; Intracytoplasmic sperm injection (ICSI); Pig

For the intracytoplasmic sperm injection (ICSI) procedure in pigs, an electrical pulse (EP) has been used as an effective method for oocyte stimulation, but unlike sperm, EP is unable to induce Ca oscillations. In this study, we investigated the effects of generating artificial Ca oscillations with...