熊果酸对TGF-β1诱导肝细胞凋亡的抑制作用及其机制

• Published in: Jie fang jun yi xue za zhi Vol. 42; no. 5; pp. 383 - 388
• Main Authors: Juan-juan, ZHOU, Wen-hua, HE, Da-kai, GAN, ZHANG, Wang, A-ping, PENG, An-jiang, WANG, Bi-min, LI, ZHU, Xuan
• Format: Journal Article
• Language: Chinese
• Published: Beijing People's Military Medical Press 01.01.2017; 330006,南昌 南昌大学第一附属医院消化内科
• Subjects: Apoptosis; Liver; Rodents; 细胞凋亡; 熊果酸; transforming growth factor β1; NADPH oxidase; 肝细胞; apoptosis; NADPH氧化酶; hepatocytes; 转化生长因子β1; ursolic acid
• ISSN: 0577-7402
• DOI: 10.11855/j.issn.0577-7402.2017.05.05

Objective To study the effect of ursolic acid (UA) intervention on hepatocyte apoptosis induced by TGF-β1 and its potential mechanism. Methods Primary hepatocytes were extracted from healthy SD rats by in situ perfusion, cultured for 12-24h, then randomly divided into the following groups: blank control group, UA control group (UA 25μmol/L), TGF-β1 group (TGF-β1 2.5ng/ml), UA intervention group (UA 25μmol/L and TGF-β1 2.5ng/ml), DPI intervention group (DPI 0.5μmol/L and TGF-β1 2.5ng/ml). Each group was treated with drugs for corresponding time and their proliferation and apoptosis were detected by flow cytometry, the expression of CD95 (Fas) mRNA was analyzed by RT-qPCR, the expression of protein CD95 and membrane translocation of NADPH oxidase (NOX) subunit p47phox were analyzed by Western blotting, and the reactive oxygen species (ROS) generation in primary hepatocytes was analyzed with reactive oxygen detection kit. Results UA intervention at 30min before TGF-β1 stimulating hepatocytes markedly reduced hep