Circadian time series proteomics reveals daily dynamics in cartilage physiology

• Published in: bioRxiv
• Main Authors: Dudek, Michal, Angelucci, Constanza, Jayalath Pd Ruckshanthi, Wang, Ping, Venkatesh Mallikarjun, Lawless, Craig, Kadler, Karl E, Hoyland, Judith A, Lamande, Shireen R, Bateman, John F, Qing-Jun, Meng
• Format: Paper
• Language: English
• Published: Cold Spring Harbor Cold Spring Harbor Laboratory Press 31.05.2019
• Subjects: Aging; Arthritis; Bioinformatics; Cartilage (articular); Cartilage diseases; Chondrocytes; Circadian rhythms; Connective tissue growth factor; Cytoskeleton; Femur; Gene expression; Glucose metabolism; Mass spectroscopy; Osteoarthritis; Protein biosynthesis; Protein turnover; Proteins; Proteomics

Objectives: Articular cartilage undergoes cyclical heavy loading and low load recovery during the 24-hour day/night cycle. We investigated the daily changes of protein abundance in mouse femoral head articular cartilage by performing 24-hour time-series proteomics study. Methods: Tandem mass spectro...