Centromeric DNA destabilizes H3 nucleosomes to promote CENP-A deposition during the cell cycle

• Published in: bioRxiv
• Main Authors: Shukla, Manu, Tong, Pin, White, Sharon A, Singh, Puneet P, Reid, Angus M, Catania, Sandra, Pidoux, Alison L, Allshire, Robin C
• Format: Paper
• Language: English
• Published: Cold Spring Harbor Cold Spring Harbor Laboratory Press 07.11.2017
• Subjects: Cell cycle; Centromeres; Chromatin remodeling; Deoxyribonucleic acid; DNA; Evictions; Histone H3; Kinetochores; Nucleosomes; Nucleotide sequence

Active centromeres are defined by the presence of nucleosomes containing CENP-A, a histone H3 variant, which alone is sufficient to direct kinetochore assembly. Once assembled at a location CENP-A chromatin and kinetochores are maintained at that location though a positive feedback loop where kineto...