Cytokine Responses during Exacerba-tion Compared with Stable Phase in Asthmatic Children

• Published in: Asian Pacific journal of allergy and immunology Vol. 28; no. 1; p. 35
• Main Authors: Charoenying, Yingwan, Kamchaisatian, Wasu, Atamasirikul, Kalayanee, Direkwattanachai, Chalerat, Manuyakorn, Wiparat, Benjaponpitak, Suwat
• Format: Journal Article
• Language: English
• Published: Bangkok The Allergy and Immunology Society 01.03.2010
• ISSN: 0125-877X

Bronchial asthma is a chronic inflammatory disorder of the airways. Balancing in Th1 and Th2 re-sponse is a target in the treatment. Recent studies show that interleukin-10 (IL-10) has an important role in the reg-ulation of Th2 and allergic responses and its amount was found to decrease in asthmatic patients. This study was to focus on cytokine responses, including interferon-gamma (IFN- γ), IL-4 and IL-10 in asthmatic children during acute exacerbation compared to stable period. Peripheral blood mononuclear cells (PBMCs) from fourteen asth-matic children during exacerbation and stable phase were stimulated with phytohemagglutinin (PHA) and mite allergen ( Der p ) for 72 hours. Levels of IFN -γ, IL-4 and IL-10 in cell culture supernatants were measured using en-zyme-linked immunosorbent assay. The median level of IL-10 in PBMCs stimulated with PHA was significantly low-er in acute asthma exacerbation compared with stable phase (464 vs . 859.5 pg/ml, p = 0.03). However, there was no difference in the level of IL-10 in PBMCs stimulated with Der p . The level of IFN-γ and IL-4 were not different between exacerbation and stable phase both in PHA and Der p -stimulated PBMCs. The decrease of IL-10 pro-duction in asthmatic children during acute exacerbation may emphasize the role of IL-10 in immune regulation in al-lergic disease.