Metabolic and cardiorespiratory responses to the lactate clamp

To evaluate the hypothesis that precursor supply limits gluconeogenesis (GNG) during exercise, we examined training-induced changes in glucose kinetics [rates of appearance (Ra) and disappearance (Rd)], oxidation (Rox), and recycling (Rr) with an exogenous lactate infusion to 3.5-4.0 mM during rest...

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Published inAmerican journal of physiology: endocrinology and metabolism Vol. 46; no. 5; pp. E889 - E898
Main Authors MILLER, Benjamin F, FATTOR, Jill A, JACOBS, Kevin A, HORNING, Michael A, SUH, Sang-Hoon, NAVAZIO, Franco, BROOKS, George A
Format Journal Article
LanguageEnglish
Published Bethesda, MD American Physiological Society 01.11.2002
Subjects
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
General aspects
Glucose
Kinetics
Metabolism
Vertebrates: anatomy and physiology, studies on body, several organs or systems
Human
Physical exercise
Physical training
Bicycle ergometer
Environmental factor
Oxygen consumption
Male
Glucose
Metabolism
Heart rate
Lactates
Young adult
Arterial pressure
Carbohydrate
Circulatory system
Hemodynamics
Kinetics
Gluconeogenesis
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Summary:To evaluate the hypothesis that precursor supply limits gluconeogenesis (GNG) during exercise, we examined training-induced changes in glucose kinetics [rates of appearance (Ra) and disappearance (Rd)], oxidation (Rox), and recycling (Rr) with an exogenous lactate infusion to 3.5-4.0 mM during rest and to pretraining 65% peak O2 consumption (O2 peak) levels during exercise. Control and clamped trials (LC) were performed at rest pre- (PRR, PRR-LC) and posttraining (POR, POR-LC) and during exercise pre- (PREX) and posttraining at absolute (POAB, POAB-LC) and relative (PORL, PORL-LC) intensities. Glucose Rr was not different in any rest or exercise condition. Glucose Ra did not differ as a result of LC. Glucose Rox was significantly decreased with LC at POR (0.38 +/- 0.03 vs. 0.56 +/- 0.04 mg x kg1 x min1) and POAB (3.82 +/- 0.51 vs. 5.0 +/- 0.62 mg x kg1 x min1). Percent glucose Rd oxidized decreased with all LC except PORL-LC (PRR, 32%; PRR-LC, 22%; POR, 27%; POR-LC, 20%; POAB, 95%; POAB-LC, 77%), which resulted in a significant increase in oxidation from alternative carbohydrate (CHO) sources at rest and POAB. We conclude that 1) increased arterial [lactate] did not increase glucose Rr measured during rest or exercise after training, 2) glucose disposal or production did not change with increased precursor supply, and 3) infusion of exogenous CHO in the form of lactate resulted in the decrease of glucose Rox.
ISSN:0193-1849
1522-1555