Identification of the Peptides that Bind to Tyrosine Kinase Receptor EphB2 by Phage Display

• Published in: Sheng wu hua hsüeh yü sheng wu wu li hsüeh pao Vol. 32; no. 5; p. 475
• Main Authors: Zhang, Xiao-Guang, Yao, Li-Bo, Han, Jiong, Liu, Xin-Ping, Han, Jing-Tian, Nie, Xiao-Yan, Su, Cheng-ZHi
• Format: Journal Article
• Language: English
• Published: China 2000

The gene encoding the Ig-like domain of tyrosine protein kinase receptor EphB2 was cloned into the expressing vector pET28a. Under induction with IPTG, the positive strain expressed the fusion protein with a hexahistidine tail on the N-terminal. The protein was purified under denaturing conditions u...