Effect of Linggui Zhugan Decoction-containing serum on mitochondrial oxidative stress in cardiomyocytes: an exploration based on Nrf2/BNIP3 signaling pathway

• Published in: Zhongguo zhongyao zazhi Vol. 47; no. 12; p. 3303
• Main Authors: Tang, Tong-Juan, Wang, Xiang, Zhou, Peng, Wang, Liang, Shi, Hui, Huang, Jin-Ling
• Format: Journal Article
• Language: Chinese
• Published: China 01.06.2022
• Subjects: Apoptosis; Mitochondrial Permeability Transition Pore; Myocytes, Cardiac; NF-E2-Related Factor 2 - genetics; NF-E2-Related Factor 2 - metabolism; Oxidative Stress; Reactive Oxygen Species - metabolism; Signal Transduction; Linggui Zhugan Decoction(LGZGD); apoptosis; reactive oxygen species(ROS); mitochondria; oxidative stress
• ISSN: 1001-5302
• DOI: 10.19540/j.cnki.cjcmm.20220111.401

This study aimed to explore the effect of Linggui Zhugan Decoction(LGZGD)-containing serum on mitochondrial oxidative stress in cardiomyocytes based on the NF-E2-related factor2(Nrf2)/Bcl-2/adenovirus E1 B 19 kDa interacting protein(BNIP3) signaling pathway. After the preparation of LGZGD-containing serum and blank serum, H9 c2 cardiomyocytes were exposed to H_2O_2 for inducing oxidative stress in vitro. The H9 c2 cells were divided into four groups, namely normal control group, H_2O_2 model group, 20% blank serum group, and 20% LGZGD-containing serum group. After the cells were pre-treated with different types of serum for 12 h and cultured with 100 μmol·L~(-1 )H_2O_2 for 6 h, the reactive oxygen species(ROS) level in each group was detected by DCFH-DA, and the openness of mitochondrial permeability transition pore(mPTP) was measured using a calcein AM fluorescent probe. The expression levels of cytoplasmic cytochrome C(CytC), mitochondrial CytC, cytoplasmic and nuclear Nrf2, and BNIP3 were detected by Weste