Preparation of mouse monoclonal antibodies against bovine TNF-α

• Published in: Xi bao yu fen zi mian yi xue za zhi Vol. 35; no. 3; p. 271
• Main Authors: Tan, Yue, Xu, Zhengzhong, Zhu, Zhaocheng, Xia, Aihong, Sun, Lin, Chen, Xiang, Jiao, Xinan
• Format: Journal Article
• Language: Chinese
• Published: China 01.03.2019
• Subjects: Animals; Antibodies, Monoclonal - immunology; Antibody Specificity; Cattle; Enzyme-Linked Immunosorbent Assay; Hybridomas; Mice; Mice, Inbred BALB C; Tumor Necrosis Factor-alpha

Objective To prepare monoclonal antibodies (mAbs) against bovine tumor necrosis factor-alpha (BoTNF-α). Methods Recombinant BoTNF-α with His tag (rHis-BoTNF-α) was expressed in a prokaryotic system as immunogen, and recombinant BoTNF-α with GST tag (rGST-BoTNF-α) was expressed as detection antigen....