Formulation and in vitro characterization of spray-dried antisense oligonucleotide to NF-kappaB encapsulated albumin microspheres

• Published in: Journal of microencapsulation Vol. 26; no. 8; pp. 692 - 700
• Main Authors: Gayakwad, Sanjay G, Bejugam, Naveen K, Akhavein, Nima, Uddin, Nasir A, Oettinger, Carl E, D'Souza, Martin J
• Format: Journal Article
• Language: English
• Published: England 01.12.2009
• Subjects: Animals; Delayed-Action Preparations - chemistry; Diffusion; Drug Compounding; Drug Delivery Systems - methods; Humans; Kinetics; Microspheres; NF-kappa B - antagonists & inhibitors; NF-kappa B - genetics; Oligonucleotides, Antisense - administration & dosage; Particle Size; Serum Albumin

The aim of this study was to formulate and characterize microspheres containing antisense oligonucleotide to NF-kappaB using bovine serum albumin as the polymer matrix. Microspheres were prepared by spray-drying technique with 5, 10 and 15% drug loading. Glutaraldehyde was used as a cross-linking ag...