Cooperation between HNF-1alpha, Cdx2, and GATA-4 in initiating an enterocytic differentiation program in a normal human intestinal epithelial progenitor cell line

• Published in: American journal of physiology: Gastrointestinal and liver physiology Vol. 298; no. 4; pp. G504 - G517
• Main Authors: Benoit, Yannick D, Paré, Fréderic, Francoeur, Caroline, Jean, Dominique, Tremblay, Eric, Boudreau, François, Escaffit, Fabrice, Beaulieu, Jean-François
• Format: Journal Article
• Language: English
• Published: United States 01.04.2010
• Subjects: Cadherins - genetics; Cadherins - metabolism; CDX2 Transcription Factor; Cell Differentiation - physiology; Cell Line; Cell Proliferation; Cyclin D1 - metabolism; Cyclin-Dependent Kinase Inhibitor p27; Dipeptidyl Peptidase 4 - genetics; Down-Regulation - genetics; Enterocytes - cytology; Enterocytes - metabolism; Enterocytes - ultrastructure; GATA4 Transcription Factor - genetics; GATA4 Transcription Factor - metabolism; Gene Expression - genetics; Gene Expression Profiling; Hepatocyte Nuclear Factor 1-alpha - genetics; Hepatocyte Nuclear Factor 1-alpha - metabolism; Homeodomain Proteins - genetics; Homeodomain Proteins - metabolism; Humans; Interphase - genetics; Intestinal Mucosa - cytology; Intestinal Mucosa - metabolism; Intestine, Small - cytology; Intestine, Small - metabolism; Intracellular Signaling Peptides and Proteins - genetics; Intracellular Signaling Peptides and Proteins - metabolism; Nerve Tissue Proteins - genetics; Nuclear Proteins - genetics; Polycomb Repressive Complex 1; Protein-Serine-Threonine Kinases - genetics; Proto-Oncogene Proteins - genetics; Receptors, G-Protein-Coupled - genetics; Repressor Proteins - genetics; RNA-Binding Proteins - genetics; Stem Cells - cytology; Stem Cells - metabolism; Stem Cells - ultrastructure; Sucrase-Isomaltase Complex - genetics; Transfection; Up-Regulation - genetics

In the intestinal epithelium, the Cdx, GATA, and HNF transcription factor families are responsible for the expression of differentiation markers such as sucrase-isomaltase. Although previous studies have shown that Cdx2 can induce differentiation in rat intestinal IEC-6 cells, no data are available...