About possible specialization of different compartments of a motoneuron axon for synthesis of specific proteins

The spontaneous quantum secretion of neurotransmitter and its regulation through the system of presynaptic acetylcholine receptors have been studied on a neuromuscular preparation of rat m. soleus of intact animals and animals in which the axonal transport was blocked via the application of colchici...

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Published inBiofizika Vol. 55; no. 5; p. 842
Main Authors Islamov, R R, Tiapkina, O V, Eremeev, A A, Shaĭmardanova, G F, Chakkaeva, E A, Kozlovskaia, I B, Nikolskiĭ, E E
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.09.2010
Subjects
Acetylcholine - secretion
Animals
Axonal Transport - physiology
Axons - metabolism
Colchicine - pharmacology
Electromyography
Exocytosis
In Vitro Techniques
Male
Membrane Potentials
Motor Neurons - metabolism
Neuromuscular Junction - metabolism
Protein Biosynthesis - drug effects
Protein Synthesis Inhibitors - pharmacology
Rats
Rats, Wistar
Receptors, Cholinergic - physiology
Receptors, Presynaptic - physiology
Sciatic Nerve - physiology
Sciatic Nerve - ultrastructure
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Summary:The spontaneous quantum secretion of neurotransmitter and its regulation through the system of presynaptic acetylcholine receptors have been studied on a neuromuscular preparation of rat m. soleus of intact animals and animals in which the axonal transport was blocked via the application of colchicine to the sciatic nerve. It was shown that, after six days of colchicine application, the spontaneous quantum secretion, the reaction of presynaptic membrane, and the reaction of neurosecretory apparatus to the depolarization of nerve endings via increase of the content of potassium ions in the environment and to the activation of presynaptic receptors by carbachol are not disturbed. Keeping in mind a rather short half-life of proteins that take part in the exocytosis and its regulation, it may be concluded that their functioning does not depend on the state of the axonal transport. These data correspond to the hypothesis put forward earlier that the synthesis of some proteins performing their function in nerve terminals occurs directly at the site of their utilization but not in the perikaryon, as it has been traditionally assumed.
ISSN:0006-3029