Methylglyoxal induces oxidative stress-dependent cell injury and up-regulation of interleukin-1beta and nerve growth factor in cultured hippocampal neuronal cells

• Published in: Brain research Vol. 1006; no. 2; p. 157
• Main Authors: Di Loreto, Silvia, Caracciolo, Valentina, Colafarina, Sabrina, Sebastiani, Pierluigi, Gasbarri, Antonella, Amicarelli, Fernanda
• Format: Journal Article
• Language: English
• Published: Netherlands 01.05.2004
• Subjects: Acetylcysteine - pharmacology; Animals; Blotting, Western - methods; Cell Death - drug effects; Cell Survival - drug effects; Cells, Cultured; Dose-Response Relationship, Drug; Drug Interactions; Embryo, Mammalian; Enzyme-Linked Immunosorbent Assay - methods; Fluoresceins; Free Radical Scavengers - pharmacology; Gene Expression Regulation - drug effects; Hippocampus - cytology; Interleukin-1 - metabolism; Nerve Growth Factor - metabolism; Neurons - drug effects; Oxidative Stress - drug effects; Oxidative Stress - physiology; Pyruvaldehyde - toxicity; Rats; Rats, Sprague-Dawley; Reactive Oxygen Species - metabolism; Reverse Transcriptase Polymerase Chain Reaction - methods; RNA, Messenger - biosynthesis; Superoxide Dismutase - metabolism; Time Factors; Up-Regulation

Methylglyoxal (MG) is one of the most powerful glycating agents of proteins and other important cellular components and has been shown to be toxic to cultured cells. Under hyperglycaemic conditions, an increase in the concentration of MG has been observed in human body fluids and tissues that seems...