Long-term soluble Abeta1-40 activates CaM kinase II in organotypic hippocampal cultures

• Published in: Neurobiology of aging Vol. 28; no. 9; pp. 1388 - 1395
• Main Authors: Tardito, Daniela, Gennarelli, Massimo, Musazzi, Laura, Gesuete, Raffaella, Chiarini, Stefania, Barbiero, Valentina Sara, Rydel, Russell E, Racagni, Giorgio, Popoli, Maurizio
• Format: Journal Article
• Language: English
• Published: United States 01.09.2007
• Subjects: Amyloid beta-Peptides - pharmacology; Animals; Animals, Newborn; Blotting, Western; Calcium - metabolism; Calcium-Calmodulin-Dependent Protein Kinase Type 2; Calcium-Calmodulin-Dependent Protein Kinases - metabolism; Enzyme Activation - drug effects; Hippocampus - drug effects; Organ Culture Techniques; Peptide Fragments - pharmacology; Phosphorylation - drug effects; Rats; Rats, Sprague-Dawley; Reverse Transcriptase Polymerase Chain Reaction - methods; RNA, Messenger - biosynthesis; Threonine - metabolism; Time Factors; Up-Regulation - drug effects

Recent findings suggested a role for soluble amyloid-beta (Abeta) peptides in Alzheimer's disease associated cognitive decline. We investigated the action of soluble, monomeric Abeta(1-40) on CaM kinase II, a kinase involved in neuroplasticity and cognition. We treated organotypic hippocampal c...