The organization of the mitotic apparatus poles in etoposide-treated CHO-K1 cells

• Published in: T͡S︡itologii͡a Vol. 50; no. 5; p. 420
• Main Authors: Balashova, E E, Riaskina, S S, Vinogradova, T M, Bystrevskaia, V B
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 2008
• Subjects: Animals; Antineoplastic Agents, Phytogenic - pharmacology; Centrioles - metabolism; CHO Cells; Cricetinae; Cricetulus; Etoposide - pharmacology; Microscopy, Fluorescence; Microscopy, Immunoelectron; Microtubules - metabolism; Microtubules - ultrastructure; Mitosis - drug effects; Spindle Apparatus - drug effects; Spindle Apparatus - metabolism; Spindle Apparatus - ultrastructure; Time Factors; Tubulin - metabolism; Tubulin - ultrastructure

In this study, we have examined the organization of the mitotic spindle poles in CHO-K1 cells dividing after treatment with the etoposide (1 h, 25 microM). We studied at various periods after the treatment: 1) the distribution of gamma-tubulin in mitotic cells by immunofluorescent staining; 2) the l...