Biosensor analysis of beta2-glycoprotein I-reactive autoantibodies: evidence for isotype-specific binding and differentiation of pathogenic from infection-induced antibodies

• Published in: Clinical chemistry (Baltimore, Md.) Vol. 53; no. 6; pp. 1137 - 1143
• Main Authors: Metzger, Jochen, von Landenberg, Philipp, Kehrel, Marcus, Buhl, Alexander, Lackner, Karl J, Luppa, Peter B
• Format: Journal Article
• Language: English
• Published: England 01.06.2007
• Subjects: Antibodies, Antiphospholipid - blood; Antigens, Bacterial - immunology; Antigens, Viral - immunology; Antiphospholipid Syndrome - immunology; Autoantibodies - blood; beta 2-Glycoprotein I - immunology; Biosensing Techniques; Cross Reactions; Enzyme-Linked Immunosorbent Assay; Humans; Immunoglobulin Isotypes - blood; Lupus Erythematosus, Systemic - immunology; Parvoviridae Infections - immunology; Parvovirus B19, Human - immunology; Protein Binding; Surface Plasmon Resonance; Syphilis - immunology; Treponema pallidum - immunology

For the laboratory diagnosis of the antiphospholipid syndrome (APS) we developed a biosensor with the ability to distinguish between disease-relevant anti-beta2-glycoprotein I (beta2GPI) autoantibodies (anti-beta2GPI) and pathogen-specific beta2GPI cross-reactive antibodies that occur transiently du...