High-field EPR study of tyrosyl radicals in prostaglandin H(2) synthase-1

• Published in: Biochemistry (Easton) Vol. 41; no. 19; pp. 6107 - 6114
• Main Authors: Dorlet, Pierre, Seibold, Steve A, Babcock, Gerald T, Gerfen, Gary J, Smith, William L, Tsai, Ah-lim, Un, Sun
• Format: Journal Article
• Language: English
• Published: United States 14.05.2002
• Subjects: Animals; Catalytic Domain; Cyclooxygenase 1; Cyclooxygenase Inhibitors - pharmacology; Electron Spin Resonance Spectroscopy; Free Radicals - chemistry; Hydrogen Bonding; In Vitro Techniques; Indomethacin - pharmacology; Isoenzymes - chemistry; Models, Molecular; Molecular Structure; Prostaglandin-Endoperoxide Synthases - chemistry; Sheep; Static Electricity; Tyrosine - chemistry

Various tyrosyl radicals generated by reaction of both native and indomethacin-inhibited ovine prostaglandin H synthase-1 with ethyl hydrogen peroxide were examined by using high-field/high-frequency EPR spectroscopy. The spectra for the initially formed tyrosyl radical commonly referred to as the &...