Binding of 14-3-3beta but not 14-3-3sigma controls the cytoplasmic localization of CDC25B: binding site preferences of 14-3-3 subtypes and the subcellular localization of CDC25B

• Published in: Journal of cell science Vol. 117; no. Pt 14; p. 3011
• Main Authors: Uchida, Sanae, Kuma, Akiko, Ohtsubo, Motoaki, Shimura, Mari, Hirata, Masato, Nakagama, Hitoshi, Matsunaga, Tsukasa, Ishizaka, Yukihito, Yamashita, Katsumi
• Format: Journal Article
• Language: English
• Published: England 15.06.2004
• Subjects: 14-3-3 Proteins - genetics; 14-3-3 Proteins - metabolism; Binding Sites; Biomarkers, Tumor - genetics; Biomarkers, Tumor - metabolism; cdc25 Phosphatases - metabolism; Cell Cycle Proteins - metabolism; Cell Nucleus - metabolism; Cells, Cultured; Exonucleases - genetics; Exonucleases - metabolism; Exoribonucleases; Humans; Mutagenesis, Site-Directed; Neoplasm Proteins - genetics; Neoplasm Proteins - metabolism; Protein Binding; Protein Transport

The dual specificity phosphatase CDC25B positively controls the G2-M transition by activating CDK1/cyclin B. The binding of 14-3-3 to CDC25B has been shown to regulate the subcellular redistribution of CDC25B from the nucleus to the cytoplasm and may be correlated with the G2 checkpoint. We used a F...